The focus of Dr. Kumar’s research includes evaluating cranial bone and muscle regeneration by harnessing muscle-derived ossification conditions. In addition, grants from the Plastic Surgery Research Foundation and the American Society of Maxillofacial Surgeons have allowed him to study heterotopic ossification in combat-related injuries and the use of harvested cells to regenerate muscle or bone in patients.
RNA-seq, rtPCR, Confocal Microscopy, Fluorescent Microscopy
Dr. Kumar leads the Craniofacial Bone Biology Laboratory at the Ross Research Building of The Johns Hopkins Univerisity. Our team is dedicated to the development of novel treatment of pathologic heterotopic bone formation and harnessing its osteogenic potential for structural bone regeneration in critical sized cranial defect models. We study muscle derived progenitor cell in both human, murine and rabbit in vitro models and in vivo murine and rabbit models. The role of bone morphogenetic proteins, inflammatory cytokines, and mechanical-transduction forces are central to our investigative methods.
Survival - Ross
Denver M. Lough, MD, PhD, Christopher Madsen, MD, Edward Swanson, MD, Devin Miller, BS, Gerald Brandacher, MD, Anand Kumar, MD. Utilization of Muscle Derived Stem Cells in Combination with hBMP-2 Seeded Scaffolding Augments Proliferation, Migration and Osteogenic Induction: A Novel Method for the Repair of Critically Sized Bone Defects. Plastic Surgery Research Council 60th Annual Meeting, Seattle, WA. Plastic & Reconstructive Surgery: May 2015 - Volume 135 - Issue 5S - p 131–132
Rotgers, SA, Meszaros, LB, Kumar, AR. Differential Effects of Inflammatory Mediators TNFα, TGFβ1 on Cellular Differentiation in a Primary Murine Muscle Cell in vitro Model of Heterotopic Ossification. Plastic & Reconstructive Surgery. March 2014; Volume 133 - Issue 3S, p67.
Rotgers, SA, Meszaros, LB, Kumar, AR. TGFβ1 is a Potent Inhibitor of BMP2 Mediated Osteogenic Differentiation in a Primary Murine Muscle Cell in vitro Model of Heterotopic Ossification. Plastic & Reconstructive Surgery. March 2014; Volume 133 - Issue 3S, p90.
Lee, Tiffany Y. BA; Meszaros, Laura B. PhD; Tobita, Kimimasa MD, PhD; Kumar, Anand R. MD. 3D Constructs of Human Skeletal Muscle-Derived Cells as a Model of Bone Formation: Osteogenic Differentiation in Response to BMP2. Plastic and Reconstructive Surgery. October 2013; Vol. 132 No. 4S-1, p147-148