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Zack Z. Wang, Ph.D.

Assistant Professor
Assistant Professor of Medicine

See Research on Pubmed



  • Assistant Professor
  • Director, Ross Flow Cytometry Core Facility
  • Assistant Professor of Medicine

Centers & Institutes

  • Cell Engineering, Institute for

Research Interests

EphB4-ephrinB2 signaling in stem cell differentiation; Molecular regulation of vascular differentiation from human ES and iPS cells; Hematopoietic development of pluripotent stem cells


Dr. Zack Wang is an Assistant Professor of Medicine in the Hematology Department.  He studies the hematopoietic development of pluripotent stem cells, molecular regulation of vascular differentiation from human ES and iPS cells, and EphB4-ephrinB2 signaling in stem cell differentiation.

Dr. Wang holds a bachelor’s degree from the East China University of Science and Technology and a PhD from the Boston University School of Medicine. He completed postdoctoral research at the Center of Regenerative Medicine at Massachusetts General Hospital and Harvard University Stem Cell Institute before joining the Johns Hopkins faculty in 2012. 


  • English
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    • Ph.D, Boston University School of Medicine, Boston, MA, Biochemistry
    • B.S., East China University of Science and Technolgoy, China, Biochemical Engineering
  • Research & Publications +

    Research Summary

    The long-term research goal of the Wang laboratory is to understand the molecular mechanisms that regulate cardiovascular and hematopoietic differentiation of pluripotent stem cells (PSCs), including embryonic stem (ES) cells and induced-pluripotent stem (iPS) cells. Pluripotent stem cells hold great potential for regenerative medicine, and gene therapy. Defining the molecular links between differentiation outcomes will provide important information for designing rational methods of stem cell manipulation.  

    Selected Publications View all on PubMed

    Wang Z, Zhang Y, Kamen D, Lees E, Ravid K.  Cyclin D3 is essential for megakaryocytopoiesis.  Blood. 1995, 86:3783-8.
    View on Pubmed

    Zhang Y, Wang Z, Ravid K.  The cell cycle in polyploid megakaryocytes is associated with reduced activity of cyclin B1-dependent cdc2 kinase.  J Biol Chem. 1996, 27:4266-72.
    View on Pubmed

    Wang Z, Sicinski P, Weinberg RA, Zhang Y, Ravid K.  Characterization of the mouse cyclin D3 gene: exon/intron organization and promoter activity.  Genomics. 1996, 35:156-63.
    View on Pubmed

    Zhang Y, Wang Z, Liu DX, Pagano M, Ravid K.  Ubiquitin-dependent degradation of cyclin B is accelerated in polyploid megakaryocytes.  J Biol Chem. 1998, 273:1387-92.
    View on Pubmed

    Wang Z, Zhang Y, Lu J, Sun S, and Ravid K.  Mpl ligand enhances the transcription of the cyclin D3 gene: a potential role for Sp1 transcription factor.  Blood. 1999, 93:4208-21.
    View on Pubmed

    Zhang Y, Sun S, Wang Z, Thompson A, Kaluzhny Y, Zimmet J, Ravid K.  Signaling by the Mpl receptor involves IKK and NF-kappaB. J Cell Biochem. 2002, 85:523-35.
    View on Pubmed

    Poznansky MC, Olszak IT, Evans RH, Wang Z, Foxall RB, Olson DP, Weibrecht K, Luster AD, Scadden DT.  Thymocyte emigration is mediated by active movement away from stroma- derived factors. J Clin Invest. 2002, 109:1101-10.
    View on Pubmed

    Wang Z, Miura N, Bonelli A, Mole P, Carlesso N, Olson DP, Scadden DT.  Receptor tyrosine kinase, EphB4 (HTK), accelerates differentiation of select human hematopoietic cells. Blood. 2002, 99:2740-7.
    View on Pubmed

    Wang Z, Shao Y., Cohen K., Mole P., Dombkowski D. and Scadden DT. Ephrin receptor, EphB4, regulates ES cell differentiation of primitive mammalian hemangioblasts, blood, cardiomyocytes, and blood vessels. Blood. 2004, 103:100-9.
    View on Pubmed

    Li ZJ, Wang Z, Zheng YZ, Xu B, Yang RC, Scadden DT and Han HC.  Kinetic Expression of Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1/CD31) during Embryonic Stem Cell differentiation. J Cell Biochem. 2005, 95:559-70.
    View on Pubmed

    Chen T, Bai H, Shao Y, Arzigian M, Janzen V, Attar E, Xie Y, Scadden DT and Wang ZZ.  Stromal cell-derived factor-1/CXCR4 signaling modifies the capillary-like organization of human embryonic stem cell-derived endothelium in vitro. Stem Cells. 2007; 25:392-401.
    View on Pubmed

    Wang ZZ#, Au P, Chen T, Shao Y, Daheron LM, Bai H, Arzigian M, Fukumura D, Jain RK#and Scadden DT#.  Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat Biotechnol. 2007; 25:317-8. (#co-corresponding authors)
    View on Pubmed

    Shao L, Feng W, Sun Y, Bai H, Liu J, Currie C, Kim J, Gama R, Wang Z, Qian Z, Liaw L, and Wu WS.  Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame. Cell Res.  2009; 19:296-306.
    View on Pubmed

    Bai H, Gao Y, Arzigian M, Wojchowski DM, Wu WS, Wang ZZ.  BMP4 regulates vascular progenitor development in human embryonic stem cells through a Smad-dependent pathway. J Cell Biochem. 2010; 109(2):363-74.
    View on Pubmed

    Sun Y, Shao L, Bai H, Wang ZZ, and Wu WS.  Slug deficiency enhances self-renewal of hematopoietic stem cells during hematopoietic regeneration. Blood. 2010; 115(9):1709-17.
    View on Pubmed

    Kuang SQ, Bai H, Fang ZH, Lopez G, Yang H, Tong WG, Wang ZZ, and Garcia-Manero G.  Aberrant DNA methylation and epigenetic inactivation of Eph receptor tyrosine kinases and ephrin ligands in acute lymphoblastic leukemia. Blood. 2010; 115(12):2412-9.
    View on Pubmed

    Shao L, Sun Y, Zhang Z, Feng W, Gao Y, Cai Z, Wang ZZ, Look AT, and Wu WS.  Deletion of proapoptotic Puma selectively protects hematopoietic stem and progenitor cells against high-dose radiation. Blood. 2010; 115(23):4707-14.
    View on Pubmed

    Jiang H, Lin X, Feng Y, Xie Y, Han J, Zhang Y, Wang ZZ, Chen T. Hemato-endothelial differentiation from lentiviral-transduced human embryonic stem cells retains durable reporter gene expression under the control of ubiquitin promoter. Cytotechnology. 2010; 62(1):31-42.
    View on Pubmed

    Chen K, Bai H, Arzigian M, Gao YX, Bao J, Wu WS, Wu L, Wang ZZ.  Endothelial cells regulate cardiomyocyte development from embryonic stem cells. J Cell Biochem. 2010; 111(1):29-39.
    View on Pubmed

    Zhang Z, Gao YX, Gordon A, Wang ZZ, Qian Z, Wu WS.  Efficient generation of fully reprogrammed human iPS cells via polycistronic retroviral vector and a new cocktail of chemical compounds. PLoS One. 2011; 6(10):e26592.
    View on Pubmed

    Bai H, Chen K, Gao YX, Arzigian M, Xie YL, Malcosky C, Yang YG, Wu WS, Wang ZZ. Bcl-xL enhances single-cell survival and expansion of human embryonic stem cells without affecting self-renewal. Stem Cell Res. 2012; 8(1):26-37.
    View on Pubmed

    Tang Y, Bai H, Urs S, Wang Z, Liaw L. Notch1 activation in embryonic VE-cadherin populations selectively blocks hematopoietic stem cell generation and fetal liver hematopoiesis. Transgenic Res. 2013; 22(2):403-10.
    View on Pubmed

    Dowell, KG, Simons AK, Wang ZZ, Yun K, Hibbs MA. Cell-Type-Specific Predictive Network Yields Novel Insights into Mouse Embryonic Stem Cell Self-Renewal and Cell Fate.  PLoS One. 2013;8(2):e56810.


    The Wang lab focuses on the signals that direct the differentiation of pluripotent stem cells, including embryonic stem cells and induced-pluripotent stem (iPS) cells, into hematopoietic and cardiovascular cells. Pluripotent stem cells hold great potential for regenerative medicine. A significant effort in the lab is underway to generate hematopoietic cells, such as megakaryocytes and platelets, from human iPS cells. Recently, Wang's team established a system to characterize the common precursors of hematopoietic and endothelial cells (hemangioblast or hemogenic endothelial cells) in human pluripotent stem cells. By transplanting endothelial cells derived from human pluripotent cells into immunodeficient mice, his researcher team showed these cells form functional blood vessels. Therefore, it is possible that endothelial cells from patient specific iPS cells could provide a cellular source for vascular regeneration in ischemic tissue. The team also investigates the niche function of endothelial cells in regulating cardiomyocyte development from pluripotent stem cells.

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    Ross Flow Cytometry

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    • Medicine - Hematology

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    Administrative Office: 410-614-0041

    Ross Reserach Building
    Room 1029
    720 Rutland Avenue
    Baltimore, MD 21205
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