Skip Navigation
  Search Menu
Find an Expert


Matthew Theodore Olson, M.D.

Photo of Dr. Matthew Theodore Olson, M.D.

Assistant Professor of Pathology


Expertise: Pathology


The Johns Hopkins Hospital (Main Entrance)

1800 Orleans St.
Sheikh Zayed Tower
Baltimore, MD 21287 map
Phone: 410-955-9790


Dr. Matthew Theodore Olson is an assistant professor of pathology at the Johns Hopkins University School of Medicine. His areas of clinical expertise include pathology with a focus in cytopathology, clinical chemistry, and bioinformatics.

He earned his M.D. from George Washington University School of Medicine. He completed his residency in anatomic and clinical pathology and a fellowship in cytopathology at The Johns Hopkins University School of Medicine.

Dr. Olson's current work focuses on the complementarity of emerging technologies with challenges in the diagnostic process of cytologic specimens and small biopsies. Defining these challenges in the diagnostic process have recently led him to lead investigations into inter-observer reproducibility, the outcome of paucicellular cytologic specimens, and nomenclature standardization in the new Paris System for reporting urinary cytology.

In laboratory endeavors, Dr. Olson focuses on methods that involve mass spectrometry, specifically those that preserve cells for analyses by multiple analyses, including conventional light microscopy. Additionally, he wrote the code for Pyromaker, an easy-to-use online computer software application that can quickly and cheaply analyze normal and mutant DNA in tumor samples to help identify meaningful gene mutations.

He is certified in anatomic and clinical pathology and in cytopathology by the American Board of Pathology. more


  • Assistant Professor of Pathology

Departments / Divisions



  • B.S., George Washington University (District of Columbia) (2003)
  • M.D., George Washington University School of Medicine and Health Science - Washington (District of Columbia) (2008)

Research & Publications

Selected Publications

Olson MT and Yergey AL. "Calculation of the Isotope Cluster for Polypeptides by Probability Grouping." J Am Soc Mass Spectrom. 2009 Feb;20(2):295-302.

Olson MT, Lombardi L, Clarke W. Clinical consequences of analytical variance and calculation strategy in oral busulfan pharmacokinetics. Clin Chim Acta 2011; 412:2316-21. PubMed PMID: 21906524.

Olson MT, Tatsas AD, Ali SZ. Cytotechnologist attended on-site evaluation of thyroid FNA adequacy: Comparison with cytopathologists and correlation with final interpretation. Am J Clin Pathol 2012; 138:90-95. PubMed PMID: 22706863.

Olson MT, Boonyaarunnate T, Han PA, Umbricht CB, Ali SZ, Zeiger MA. A tertiary center's experience with second review of 3,885 thyroid cytopathology specimens. J Clin Endocrinol Metab 2013; 98:1450-57. PubMed PMID: 23436916.

Olson MT, Harrington C, Beierl K, Chen G, Thiess M, O'Neill A, Taube JM, Zeiger MA, Lin M-T, Eshleman JR. BRAF pyrosequencing analysis aided by a lookup table. Am J Clin Pathol 2014; 141: 639-647.

Olson MT, Baxi A, ElNaggar M, Umbricht C, Yergey AL, Clarke W. Morphologically compatible mass spectrometric analysis of lipids in cytological specimens. J Am Soc Cytol 2016; 5: 3-8.

Is this you? Edit Profile