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Elias Thomas Zambidis, M.D., Ph.D.

Associate Professor of Oncology

Male  | Languages: English, Greek

Appointment Phone


Main Location

Johns Hopkins Sidney Kimmel Comprehensive Cancer Center

Out-of-State & International Patients +
Out of State Patients

Call 410-464-6641 (8a.m. to 6p.m., EST, Mon-Fri)

Learn more about our out-of-state patient services »

International Patients

Call +1-410-502-7683 (7a.m. to 6p.m., EST, Mon-Fri)

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  • Associate Professor of Oncology
  • Assistant Professor of Pediatrics

Centers & Institutes



Johns Hopkins Sidney Kimmel Comprehensive Cancer Center

Appointment Phone: 443-287-6997

401 N. Broadway
Charlotte R. Bloomberg Childrens Center
Baltimore, MD 21231 map
Fax: 410-955-0028

Contact for Research Inquiries

Phone: 443-287-2949


Acute Lymphoblastic Leukemia, Acute Myeloid Leukemia, Chronic Myeloid Leukemia , Hodgkin's Disease , Leukemia, Myelodysplastic Syndrome, Non-Hodgkin's Lymphoma, Pediatric Hematology-Oncology, Pediatric Oncology, Stem Cell Transplants

Featured Video

Elias Zambidis - Using Stem Cells to Treat Blood Disorders

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    Additional Information

  • Education +


    • University of Rochester / MD PhD (1998)


    • St. Louis Children's Hospital / Pediatrics (2001)


    • Johns Hopkins University School of Medicine / Pediatric Hematology & Oncology (2004)

    Additional Training

    • Diplomate, American Board of Pediatrics (2001)
  • Research & Publications +

    Research Summary

    The Zambidis lab is interested in the developmental biology of normal and malignant human stem cells. His group employs genetic manipulation and differentiation of human embryonic and induced pluripotent stem cells (iPSC) to study the cellular and molecular mechanisms of human hematopoiesis, vasculogenesis, and cardiogeneisis. Using human embryonic stem cells (hESC) derived from both normal and preimplantation genetic diagnosis (PGD)-screened embryos, as well as human iPSC, he is exploring how early mesodermal progenitors and human hemangioblast (bi-potential progenitor of hematopoietic stem cells (HSC) and endothelium) may give rise to the entire human hematopoietic and vascular systems, and whether such progenitors can be derived and expanded from differentiating human iPSC. His laboratory is studying the role of a variety of proteins and signaling molecules that are critically important in orchestrating the initiation of human embryonic hematopoiesis by directing the formation of human hemato-vascular progenitors in hESC and hiPSC. hESC/hiPSC-derived blood progenitors are also important in the understanding of the developmental origins of pediatric leukemia, but also for clinical HSC transplantation. Another major area of focus is determining the shared molecular circuits that regulate both malignant transformation and the maintenance of pluripotency. Applying these principles of shared biology between hematopoietic stem-progenitors and pluripotent stem cells, his group recently accomplished the creation of improved, highly efficient methods for generating nonintegrated, non-viral human induced pluripotent stem cell (hiPSC) lines from myeloid progenitors that possess enhanced differentiation capacities and rapid loss of epigenetic memory. Additional projects include studying the developmental biology of gastrulation and cardiogenesis in model organisms and explores potential applications of pluripotent stem cell-derived cardiac in tissue engineering, regenerative medicine, cardiotoxicity screening and novel drug discovery.

    Technology Expertise: Histiocytic Disorders, Stem Cell Transplantation (blood disorders)


    The Zambidis Lab

    Selected Publications

    Zambidis E, Peault B, Park TS, Bunz F, Civin CI. Hematopoietic differentiation of human embryonic stem cells progresses through sequential hemato-endothelial, primitive, and definitive stages resembling human yolk sac development. Blood, 2005; 106: 860-70.

    Zambidis E, Oberlin E, Tavian M, Peault B. Blood-forming endothelium in human ontogeny: lessons from in utero developmental and embryonic stem cell culture studies. Trends in Card. Med., 2006, 0:1-8.

    Zambidis E, Sinka L, Tavian M, Jokubaitis V, Simmons P, Péault B. (2007). Emergence of human angio-hematopoietic cells in normal development and from cultured embryonic stem cells, Ann NY Acad Sci., 1106: 223-32.

    Zambidis E,Park TS, Yu W, Tam A, Levine M, Yuan X, Pryzhkova M, Peault B. (2008). Expression of ACE (CD143) identifies and regulates primitive hemangioblasts derived from human pluripotent stem cells, Blood, 112: 3601-14.

    Peters A, Burridge P, Levine M, Pryzhkova M, Park TS, Yuan X, Zambidis E. (2010). Challenges in generating therapeutic patient-specific hemangioblasts and hematopoietic stem cells from human pluripotent stem cells. Int. J. Developmental Biology, 54: 965-90.

    Ohm J, Mali P, van Nester L, Berman D, Liang L, Pandiyan K, Briggs K, Zhang W, Argani P, Sioms B, Zambidis E. Schuebel K, Cope L, Yen J, Cheng L, Baylin S. (2010). Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells, Cancer Res., 19: 7662-73.

    Burridge PW, Thompson S, Millrod MA, Weinberg S, Yuan X, Tung L, Zambidis E. (2011). A universal protocol for highly efficient cardiac differentiation of human pluripotent stem cells that eliminates interline variability. PLoS One, 6(4): e18293. doi:10.1371/journal.pone.0018293.

    Thompson S, Burridge P, Lipke E, Zambidis E,Tung LT, (2012). Engraftment of human embryonic stem cell-derived cardiomyocytes improved conduction in an arrythmogenic in vitro model. J. Mol. Cell. Cardiology, 53: 15-23.

    Park TS, Zimmerlin L, Zambidis E. (2012). Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells. Cytometry A, 83: 114-126. DOI: 10.1002/cyto.a.22090.

    Park TS, Huo JS, Peters A, Talbot CC, Kaplan IM, Zambidis E. (2012). Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate iPSC reprogramming of lineage-committed human myeloid progenitors, PLoS One, 7(8): e42838. DOI:10.1371/journal.pone.0042838.

    Blazeski A, Zhu R, Hunter DW, Weinberg S, Zambidis E, Tung L. (2012). Electrophysiological and contractile function of cardiomyocytes derived from human embryonic stem cells. Prog. Biophys & Mol. Biology, 110(2-3):178-95.

    Blazeski A, Zhu R, Hunter DW, Weinberg S, Zambidis E, Tung, L. (2012). Cardiomyocytes derived from human induced pluripotent stem cells for modeling normal and diseased cardiac electrophysiology and contractility. Prog. Biophys & Mol. Biology, 110(2-3):166-77.

    Huo JS, Zambidis E. (2012). Pivots of pluripotency: the roles of non-coding RNAs in regulating embryonic and induced pluripotent stem cells. Biochim. Biophys. Acti, 1830 (2): 2385-94.

    Panicker LM, Miller D, Park TS, Patel B, Azevedo JL, Awad O, Masood AM, Veenstra TD, Goldin E, Polumuri SK, Vogel SN, Sidransky E, Zambidis ET, Feldman RA. (2012). Modeling Gaucher disease using human induced pluripotent stem (iPS) cells. Proc. Natl. Acad. Sci. USA, 109(44):18054-9.

    Resar LMS, Sha SN, Kerr C, Cope L, Zambidis E, Belton A, Huso D. (2012). HMGA1 a factor enriched in hematopoietic stem cells, embryonic stem cells and hematologic malignancy enhances cellular reprogramming to a pluripotent stem-like cell, PLoS One, 7(11):e48533.

    Rufaihah AJ, Huang NF, Kim J, Herold J, Volz K, Park TS, Lee JC,Zambidis E. Reijo-Pera, R, and JP Cooke. (2013). Human induced pluripotent stem cell-derived endothelial cells exhibit functional heterogeneity, Am J Transl Res, 5 (1): 21-35.

    Park, TS, Bhutto, I, Zimmerlin, L, Huo, JS, Nagaria, Miller, D, Rufaihah, AJ, Talbot, CC, Aguilar, J, Grebe, R, Merges, Reijo-Pera, R, J, Feldman, Rassool, F, C, Cooke, Lutty, G, and E Zambidis. (2014). Vascular progenitors from cord blood-derived iPSC possess augmented capacity to regenerate ischemic retinal vasculature. Circulation, 129: 359-72.

    Gorospe G, Zhu R, Millrod MA, Zambidis ET, Tung L, Vidal R. (2014). Automated grouping of action potentials of embryonic stem cell-derived cardiomyocytes. 99: IEEE Trans. Biomed. Eng, 2014.

    Panicker LM, Miller D, Awad O, Bose V, Lun Y, Park TS, Zambidis ET, Sgambato, JL, and RA Feldman. (2014). Activated Gaucher iPSC macrophages are a new platform for therapeutic development, Stem Cells, 2014.

    ZimmerlinL, Park TS, Donnenberg VS, Zambidis E, Donnenberg AD. (2014). Pericytes: a ubiquitous source of adult tissue stem cells, in "Stem Cells in Aesthetic Procedures", edited by Melvin Shiffman, Springer, 2014 (Book Chapter).

    Agarwal JR, Zambidis ET. (2014). Role of an NFkB-STAT3 signaling axis in the induction and maintenance of the pluripotent state. In Press, InTech, 2014.

    Park TS, Donnenberg VS, Donnenberg AD, Zambidis E, Zimmerlin L.(2014). Dynamic Interactions Between Cancer Stem Cells And Their Stromal Partners. Curr Pathobiol Rep. DOI 10.1007/s40139-013-0036-5.

    Zhong X, Gutierrez C, Xue T,Hampton C, Vergara MN, Peters A,Zambidis E, Meyer J, Gamm DM, Yau KW, Canto-Soler MV. (2014). Generation of three-Dimensional retinal cups with mature functional photoreceptors from human iPSC, Nature Com, 2014.

  • Academic Affiliations & Courses +

    Graduate Program Affiliation

    Cellular and Molecular Medicine

  • Activities & Honors +


    • International Society of Stem Cell Research, 2006
    • American Society of Hematology, 2004
    • American Association of Pediatrics, 2001
    • American Society of Clinical Oncology, 2004 - 2008
  • Videos & Media +
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