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Charles George Eberhart, M.D., Ph.D.
Director, Neuropathology and Ophthalmic Pathology
Professor of Pathology
Languages: English, German
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The Johns Hopkins Hospital
Appointment Phone: 410-955-5568
600 N. Wolfe Street
Sheikh Zayed Tower
Baltimore, MD 21287 map
Dr. Charles Eberhart is a professor of pathology and oncology, and an associate professor of ophthalmology, at the Johns Hopkins University School of Medicine. His research primarily focuses on molecular genetics and treatment of medulloblastoma, glioblastoma, uveal melanoma and other tumors of the brain and eye. Dr. Eberhart serves as the director of the Divisions of Neuropathology and Ophthalmic Pathology.
Major research areas in his laboratory include targeting Notch and other developmentally important signaling pathways in tumors, identifying the genetic drivers of rare neoplasms in the brain and eye, and building new models of tumors using neural stem cells.
He received his undergraduate degree in biochemistry from the University of Texas at Austin and earned his M.D./Ph.D. from UT Southwestern Medical School. He also was a fellow at the Max Planck institute for one year. Dr. Eberhart completed both a residency in anatomic pathology and a fellowship in neuropathology at The Johns Hopkins Hospital, then joined the Johns Hopkins faculty in 2001.
Dr. Eberhart has been recognized with the Howard Hughes Medical Institute Postdoctoral Research Fellowship for Physicians, and career development awards from the Burroughs Wellcome Fund and the National Institutes of Health. He has published more than 200 original research articles, numerous case reports, reviews and book chapters, and is on the editorial board of the Journal of Neuro-Oncology, Brain Pathology and the Journal of Neuropathology and Experimental Neurology.
- Director, Neuropathology and Ophthalmic Pathology
- Professor of Pathology
- Associate Professor of Ophthalmology
- Professor of Oncology
Centers & Institutes
- MD PhD, University of Texas Southwestern Medical School (1997)
- Johns Hopkins University School of Medicine / Pathology (2001)
- American Board of Pathology / Anatomic Pathology (2003)
- American Board of Pathology / Neuropathology (2003)
Research & Publications
Dr. Eberhart and his lab members study the molecular genetics of medulloblastoma, low grade glioma, glioblastoma, uveal melanoma and other tumors of the brain and eye. One major focus is on Notch and other pathways which play a key role in both normal development and in neoplasia. They are currently analyzing the long terms effects of Notch blockade on cancer stem cells in brain and eye tumors, how therapeutic resistance emerges, and the basis of the sometimes antagonistic effects of various Notch receptors. They are also examining the role of c-Myc in the stem cell phenotype of cancer. Identifying novel genetic changes in ocular surface tumors and other poorly understood neoplasms represents another focus.
Dr. Eberhart works closely with Drs.Rodriguez and Raabe in a multidisciplinary research group. Their studies use our understanding of normal development to guide investigation of the neoplastic process. They have implicated Hedgehog, Notch and other developmentally significant signaling cascades in the initiation and ongoing growth of brain tumors. They are also engaged in preclinical testing of pharmacological agents that target multiple pathways in brain and eye tumors. To accomplish this they have generated new tumor models from surgical specimens and by making relevant oncogenic changes in progenitor cells. A related area of interest in the lab is the relationship between stem cells and cancer. The possibility that specific types of transformed stem cells represent the origins of brain and eye tumors, as well as the hypothesis that “cancer stem cells” are required for long-term tumor self renewal and growth, are both active areas of investigation.
Raabe EH, Lim KS, Kim JM, Meeker A, Mao XG, Nikkhah G, Maciaczyk J, Kahlert U, Jain D, Bar E, Cohen KJ, Eberhart CG. BRAF Activation Induces Transformation and Then Senescence in Human Neural Stem Cells: A Pilocytic Astrocytoma Model. Clin Cancer Res. 2011;17(11):3590-9
Pierfelice TJ, Schreck KC, Dang L, Asnaghi L, Gaiano N, Eberhart CG. Notch3 Activation Promotes Invasive Glioma Formation in a Tissue Site-Specific Manner. Cancer Res 2011; 71;1115-25.101.
Chu Q, Orr BA, Semenkow S, Bar EE, Eberhart CG. Prolonged Inhibition of Glioblastoma Xenograft Initiation and Clonogenic Growth following In Vivo Notch Blockade. Clin Cancer Res 2013;19(12):3224-33.
Asnaghi L, Lin MH, Lim KS, Lim KJ, Tripathy A, Wendeborn M, Merbs SL, Handa JT, Sodhi A, Bar EE, Eberhart CG. Hypoxia promotes uveal melanoma invasion through enhanced Notch and MAPK activation. PLoS One. 2014;9(8):e105372
Hanaford AR, Archer TC, Price A, Kahlert UD, Maciaczyk J, Nikkhah G, Kim JW, Ehrenberger T, Clemons PA, Dančík V, Seashore-Ludlow B, Viswanathan V, Stewart ML, Rees M, Shamji AF, Schreiber SL, Fraenkel E(, Pomeroy SL, Mesirov JP, Tamayo P, Eberhart CG*, Raabe EH* (*corresponding authors). DiSCoVERing innovative therapies for rare tumors: combining genetically accurate disease models with in silico analysis to identify novel therapeutic targets. Clin Cancer Res. 2016
Academic Affiliations & Courses
Graduate Program Affiliation
Activities & Honors
- National Merit Scholar, Phi Beta Kappa, University of Texas at Austin
- Deutches Akademisches Austauschdienst Graduate Fellow, 1988 - 1989
- March of Dimes Graduate Fellow, 1992 - 1994
- Merck MSTP Fellow, 1996 - 1997
- Howard Hughes Postdoctoral Research Fellowship for Physicians, 2000 - 2003
- Burroughs Wellcome Fund Career Award, 2001 - 2005
- Jean D Wilson Distinguished Alumni Lecturer, UT Southwestern MSTP, 2004
- Lucien J. Rubenstain Award for Best Paper in Neuro-oncology, AANP Meeting, 2005
- Elected to American Society for Clinical Investigation, 2010
- Elected to American Association of University Pathologists (Pluto Society), 2015
- American Association for Cancer Research
- American Association of Neuropathology
- Association for Research in Vision and Ophthalmology
- Eastern Cooperative Oncology Group
- Society for Neuro-Oncology
- Verhoeff Zimmerman Society
- American Association of Ophthalmic Oncology and Pathology
- United States and Canadian Academy of Pathology
- International Society for Ocular Oncology
- Children’s Oncology Group
Member of CNS Disease Committee and Co-director of Low Grade Astrocytoma Subcommittee
- Abstract Review for Scientific Program, Society for Neurooncology (SNO), 2006
- Classification of Tumours of the Nervous System Editorial and Consensus Conference, WHO, 2006
Heidelberg Invited Participant and Group D Rapporteur
- Editorial board, Journal of Neuropathology and Experimental Neurology, 2005
- Editorial board, Neuro-Oncology, 2006
- NIH CAMP Study Section Member, 2016