S. Diane Hayward

Department Affiliation: Primary: Oncology; Secondary: Pharmacology and Molecular Sciences, Neurology
Degree: Ph.D., University of Otago, New Zealand
Rank: Professor
Telephone Number: 410-955-2548
Fax Number: 410-502-6802
E-mail address: dhayward@jhmi.edu
School of Medicine Address: Bunting-Blaustein Cancer Research Building, CRBI 308;1650 Orleans Street, Baltimore, MD 21231

Herpesvirus-Host Cell Interactions.

Epstein-Barr virus (EBV) and Kaposi’s sarcoma associated herpesvirus (KSHV) are human herpesviruses that are associated with a variety of malignancies. We seek to understand the genesis of virus-associated cancers by examining the mechanism of action of virus proteins that interact with cell signaling pathways to promote cell proliferation and cell survival. The laboratory also examines the regulation of lytic EBV and KSHV replication. Lytic replication is necessary for virus spread to new hosts and unregulated virus replication is a risk factor for the development of EBV and KSHV associated cancers. In collaboration with the Zhu laboratory we are developing herpesvirus proteomic arrays as new tools for probing virus-cell interactions. The protein arrays are being used to identify post-translational modifications such as phosphorylation and sumolation that modify virus protein functions. The ability of viral kinases to modify host protein functions is also being investigated using human protein arrays to identify viral kinase substrates.

Representative Publications:

Shamay, M., Krithivas, A., Zhang, J., and Hayward, S.D. Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi's sarcoma-associated herpesvirus LANA. Proc. Nat. Acad. Sci. USA 103: 14554-14559, 2006. Pub Med Reference

Martin, H.J., Lee, J.M., Walls, D., and Hayward, S.D. Manipulation of the toll-like receptor 7 signaling pathway by Epstein-Barr virus. J. Virol. 81:9748-9758, 2007. Pub Med Reference

Liu, J., Martin, H.J., Liao, G., and Hayward S.D. The Kaposi's sarcoma-associated herpesvirus LANA protein stabilizes andactivates c-Myc. J. Virol. 81:10451-10459, 2007. Pub Med Reference
Lo, A.K., To, K.F., Lo, K.W., Lung, R.W., Hui, J.W., Liao, G., and Hayward S.D. Modulation of LMP1protein expression by EBV-encoded microRNAs. Proc. Nat. Acad. Sci. USA 104:16164-16169, 2007. Pub Med Reference

Zhu, J., Liao, G., Shan, L., Zhang, J., Chen, M.R., Hayward, G.S., Hayward, S.D., Desai, P., and Zhu, H. Protein array identification of substrates of the Epstein-Barr virus protein kinase BGLF4. J. Virol. 83:5219-5231, 2009. Pub Med Reference
Shamay, M., Greenway, M., Liao, G., Ambinder, R.F., and Hayward, S.D. De novo DNA methyltransferase DNMT3b interacts with NEDD8-modified proteins. J. Biol. Chem. 285:36377-86, 2010. Pub Med Reference
Zheng, D., Wan, J., Cho, Y.G., Wang, L., Chiou, C.-J., Pai, S., Woodard, C., Zhu, J., Liao, G., Martinez-Maza, O., Qian, J., Zhu, H., Hayward, G.S., Ambinder, R.F., and Hayward, S.D. Comparison of humoral immune responses to Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray. J. Infect. Dis. In press, 2011.
Li, R., Zhu, J., Xie, Z., Liao, G., Liu, J., Chen, M.-R., Hu, S., Woodard, C., Lin, J., Taverna, S., Desai, P., Ambinder, R.F., Hayward, G.S., Qian, J., Zhu, H., and Hayward, S.D. Conserved herpesvirus kinases regulate TIP60 to promote virus replication. Cell Host Microbe. In press, 2011.