Department Affiliation: Primary: Medicine, Division of Infectious Diseases; Secondary: Pharmacology and Molecular Sciences
Degree: M.D., Universidade Federal de Pernambuco - Brazil; Ph.D., The Johns Hopkins University School of Medicine
Rank: Assistant Professor
Telephone Number : 410-955-8477
Fax Number: 410-955-1894
E-mail address: emarques@jhmi.edu
School of Medicine Address: 307 Biophysics Building, 725 N. Wolfe St., Baltimore, MD 21205
Vaccine design and immunotherapies; immunomics; immunopathogenesis of Dengue and HIV
The main focus of our laboratory is the design of novel vaccines, immunotherapies and diagnostic markers based correlates of protection and pathogenesis identified in samples from well characterized patient cohorts. Detailed maps of T and B cell epitopes and molecules of the innate immune response (immunomes) are built using the combination of computational technologies, high throughput immune assays with human samples and suitable animal models. Through the immunome maps, correlates of immunity and pathogenicity are investigated and novel antigen formulations containing selected epitopes and innate immune stimulus are proposed as candidate vaccines. Currently we are applying this strategy to study Dengue virus, Yellow Fever, HIV, Hepatitis A virus, Hantavirus, Rabies and Arena virus infections. Two of ours leading vaccine candidates are going to be tested in human clinical studies in 2008/2009.
Representative Publications:
Cordeiro MT, Silva AM, Brito CA, Nascimento EJ, Magalhães MC, Guimarães GF, Lucena-Silva N, de Carvalho EM, Marques ET Jr.
Characterization of a dengue patient cohort in Recife, Brazil. Am J Trop Med Hyg. 2007 Dec;77(6):1128-34. Pub Med Reference.Arruda LB, Sim D, Chikhlikar PR, Maciel M Jr, Akasaki K, August JT, Marques ET. Dendritic cell-lysosomal-associated membrane protein (LAMP) and LAMP-1-HIV-1 gag chimeras have distinct cellular trafficking pathways and prime T and B cell responses to a diverse repertoire of epitopes. J Immunol. 2006 Aug 15;177(4):2265-75. Pub Med Reference.
Braga-Neto UM, Marques ET Jr. From functional genomics to functional immunomics: new challenges, old problems, big rewards.
PLoS Comput Biol. 2006 Jul 28;2(7):e81. Pub Med Reference.Marques ET Jr, Chikhlikar P, de Arruda LB, Leao IC, Lu Y, Wong J, Chen JS, Byrne B, August JT. HIV-1 p55Gag encoded in the lysosome- associated membrane protein-1 as a DNA plasmid vaccine chimera is highly expressed, traffics to the major histocompatibility class II compartment, and elicits enhanced immune responses. J Biol Chem. 2003 Sep 26;278(39):37926-36. Epub 2003 Jun 24. Pub Med Reference.
Lu Y, Raviprakash K, Leao IC, Chikhlikar PR, Ewing D, Anwar A, Chougnet C, Murphy G, Hayes CG, August TJ, Marques ET Jr. Dengue 2 PreM-E/LAMP chimera targeted to the MHC class II compartment elicits long-lasting neutralizing antibodies. Vaccine. 2003 May 16;21(17-18): 2178-89. Pub Med Reference.
Other graduate programs in which Dr. Marques participates:
