Department Affiliation: Primary: Medicine, Division of Infectious Diseases; Secondary: Pharmacology and Molecular Sciences
Degree: M.D., Universidade Federal de Pernambuco - Brazil; Ph.D., The Johns Hopkins University School of Medicine
Rank: Assistant Professor
Telephone Number : 410-955-8477
Fax Number: 410-955-1894
E-mail address: emarques@jhmi.edu
School of Medicine Address: 307 Biophysics Building, 725 N. Wolfe St., Baltimore, MD 21205
Vaccine design and immunotherapies; immunomics; immunopathogenesis of Dengue and HIV
The mission of the research in our laboratory is the development of vaccines, immunotherapy and diagnostic markers based on the study of immunomes and the characterization of their correlates of immune protection and pathogenesis in well characterized clinical cohorts. "The immunome is the complete detailed map of immune reactions of a given host interacting with a foreign antigen, and immunomics is the study of immunomes." Whereas functional genomics strives to identify the role of genes in cellular processes via the paradigm of hybridization of mRNA to complementary DNA, functional immunomics aims to identify the roles of chemical/biological targets involved in immunological processes via the paradigm of specific cellular and humoral immune responses elicited by antigens presented to the immune system. This is an effort that promises great rewards, both in terms of our basic understanding of the immune system and in terms of disease diagnosis/prognosis and the design of vaccines to combat a variety of human infirmities ranging from pathogenic infections to allergies and cancer. Using high throughput immune assays technologies we are currently characterizing the immunomes of Dengue virus, Yellow Fever, HIV, Hepatitis A virus, Hantavirus, Rabies and Arena virus in clinical cohorts and studying possible their correlates of protection and disease development in order to design better vaccines.
Representative Publications:
Chikhlikar, P., Barros de Arruda, L., Agrawal, S., Byrne, B., Guggino, W., August, J.T., and Marques, E.T. Inverted terminal repeat sequences of adeno-associated virus enhance the antibody and CD8(+) responses to a HIV-1 p55Gag/LAMP DNA vaccine chimera, Virology 323:220-232, 2004. Pub Med Reference
Arruda, L.B., Chikhlikar, P.R., August, J.T., and Marques, E.T. DNA vaccine encoding human immunodeficiency virus-1 Gag, targeted to the major histocompatibility complex II compartment by lysosomal-associated membrane protein, elicits enhanced long-term memory response, Immunology 112:126-133, 2004. Pub Med Reference
Lu, Y., Raviprakash, K., Leao, I.C., Chikhlikar, P.R., Ewing, D., Anwar, A., Chougnet, C., Murphy, G., Hayes, C.G., August, J.T., and Marques, E.T. Dengue 2 PreM-E/LAMP chimera targeted to the MHC class II compartment elicits long-lasting neutralizing antibodies, Vaccine 21:2178-2189, 2003. Pub Med Reference
Marques, E.T., Chikhlikar, P., de Arruda, L.B., Leao, I.C., Lu, Y., Wong, J., Chen, J.S., Byrne, B., and August, J.T. HIV-1 p55Gag encoded in the lysosome-associated membrane protein-1 as a DNA plasmid vaccine chimera is highly expressed, traffics to the major histocompatibility class II compartment, and elicits enhanced immune responses, J. Biol. Chem. 278:37926-37936, 2003. Pub Med Reference
Marques, E.T., Ichikawa, Y., Strand, M., August, J.T., Hart, G.W., and Schnaar, R.L. Fucosyltransferases in Schistosoma mansoni development, Glycobiology 11:249-259, 2001. Pub Med Reference
Other graduate programs in which Dr. Marques participates:
