Department Affiliation: Primary: Oncology; Secondary: Pharmacology and Molecular Sciences
Degree: Ph.D., University of Otago, New Zealand
Rank: Professor
Telephone Number: 410-955-2548
Fax Number: 410-502-6802
E-mail address: dhayward@jhmi.edu
School of Medicine Address: Room 308 Bunting-Blaustein Cancer Research Building, 1650 Orleans Street, Baltimore, MD 21231
Epstein-Barr virus and Kaposi’s sarcoma associatedherpesvirus; viral latency and tumorigenesis; mechanisms of virus-induced cellproliferation; herpesvirus proteomics
Epstein-Barr virus (EBV) and Kaposi’s sarcoma associated herpesvirus(KSHV or HHV-8) establish latent infections in B cells but also infect epithelial(EBV) and endothelial (KSHV) cells. EBVis associated with B cell lymphoma, Hodgkin’s disease and carcinoma of thenasopharynx and KSHV with Kaposi’s sarcoma and primary effusion lymphoma inAIDS patients.
The EBV EBNA2 protein mimics aspects of cellular Notchsignaling while the KSHV LANA protein inactivates the serine/threonine kinaseGSK-3 to stabilize beta-catenin and mimic aspects of Wnt pathwaysignaling. LANA also regulates cell geneexpression through interactions with DNA methyltransferases and methyl CpGbinding proteins. We are persuingproteomic approaches to further explore the functions of EBNA2 and LANA and todevelop virus protein-cell protein interaction networks. A pan herpesvirus proteomic chip containingthe proteins encoded by EBV, KSHV, HSV-1, HCMV is being developed. Interactions between EBNA2 and LANA and humangenomic protein arrays and human protein kinase arrays are being performed toidentify novel functional partners for these key viral proteins.
Representative Publications:
- Fujimuro M., Wu F.Y., ApRhys C., Kajumbula H., Young D.B., Hayward G.S., and Hayward S.D. A novel viral mechanism for dysregulation of beta-catenin in Kaposi’s sarcoma-associated herpesvirus latency. Nat. Med. 9:300-306, 2003. Pub Med. Reference
- Farrell C. J., Lee J. M., Shin E. C., Cebrat M., Cole P. A., and Hayward S. D. Inhibition of Epstein-Barr virus-induced growth proliferation by a nuclear antigen EBNA2-TAT peptide. Proc. Nat. Acad. Sci. USA 101:4625-4630, 2004. Pub Med Reference
- Shamay M., Krithivas A., Zhang J. and Hayward S.D. Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi'ssarcoma-associated herpesvirus LANA. Proc. Nat. Acad. Sci. USA 103: 14554-14559, 2006. Pub Med Reference
Liu J., Martin H.J., Liao G. and Hayward S.D. The Kaposi's sarcoma-associated herpesvirus LANA protein stabilizes andactivates c-Myc. J. Virol. 81: 10451-10459, 2007. Pub Med Reference
- Lo A.K., To K.F., Lo K.W., Lung R.W., Hui J.W., Liao G. and Hayward S.D. Modulation of LMP1protein expression by EBV-encoded microRNAs. Proc. Nat. Acad. Sci. USA 104:16164-16169, 2007. Pub Med Reference
Other graduate programs in which Dr. Hayward participates:
Biochemistry, Cellular and Molecular Biology
Anti-Cancer Drug Development Program
Cellular and Molecular Medicine
