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Rafael Guerrero-Preston, DrPH, MPH

Rafael Guerrero-Preston, DrPH, MPH

Rafael Guerrero-Preston DrPH, MPH

Assistant Professor of Otolaryngology–Head and Neck Surgery and Oncology


EDUCATION – Fellowship
Basic Medical Science: Mount Sinai School of Medicine
Cancer Prevention Fellowship: Columbia University Mailman School of Public Health
Cancer Epigenomics and Genomics: Johns Hopkins School of Medicine

EDUCATION – Graduate School
University of Puerto Rico School of Public Health

EDUCATION – Undergraduate
Columbia University


  • Translational epigenomics
  • Develop biomonitors for cancer early detection, clinical management and therapeutic development
  • Discover and validate molecular markers of HPV disease
  • Discover and validate molecular markers and biomonitors for cancer health disparities research


[1]  Guerrero-Preston R., Santella R., Blanco A., Desai M., Berdasco M., Fraga M. (2007) Global DNA hypomethylation in liver cancer cases and controls: A Phase I biomarker development study. Epigenetics, 2(4):223-6. PMID: 18032927

[2] Guerrero-Preston R., Vlahov D., Chan C., Nash D., Mitchell M., Johnson S., Freeman H. (2008).  Previous cancer screening behavior as predictor of colon cancer screening among women aged 50 and over, in NYC 2002. J Community Health, 33(1):10-21. PMID: 18080204

[3] Guerrero-Preston R. (2008) Global epigenetic screening technologies: A novel tool to address cancer health disparities in high-risk population groups.  Puerto Rico Health Sciences Journal, 27(4):350-356. PMID: 19069364

[4] Guerrero-Preston R, Báez A, Blanco A, Berdasco M, Fraga M, M, Esteller. (2009) Global DNA methylation: a common early event in oral cancer cases with exposures to environmental carcinogens or viral agents. Puerto Rico Health Sciences Journal, 28(1):24-29. PMID: 19266736

[5] Loyo M, Guerrero-Preston  R, Brait M, Hoque MO, Chuang A, Kim MS, Liegeois NJ, Koch WM, Califano J, Westra WH, Sidransky D. (2009) Quantitative detection of Merkel Cell Virus in human tissues and possible mode of transmission. International Journal of Cancer 126(12):2991-6. PMID:19588496

[6]  Kim MS, Louwagie J, Carvalho B,  Droste J, Park HL, Chae YK, Yamashita K, Liu J, Ostrow K, Ling S, Guerrero-Preston  R, Demokan S, Yalniz Z, Dalay N, Van Criekinge W, Sidransky D. (2009).  Promoter DNA methylation of Oncostatin M receptor-β as a novel diagnostic and therapeutic marker in colon cancer. PLoS One 7;4(8):e6555. PMID: 19662090

[7] Guerrero-Preston R., Siegel A, Renz J,  Vlahov D, Neugut A. (2009)  HCV infection and cryptogenic cirrhosis are risk factors for Hepatocellular carcinoma among Latinos in New York City, Journal of Community Health, 34(6):500-505. PMID: 19705261

[8]Guerrero-Preston R, Goldman L, Brebi-Mieville P, Ili-Gangas C, LeBron L, Hernandez-Arroyo M, Witter FR, Apelberg BJ, Roystacher M, Jaffe A, Halden R, and Sidransky D. (2010) Global DNA hypomethylation is associated with in utero exposure to cotinine and perfluorinated alkyl compounds. Epigenetics, 5(6):539-546. PMID: 20523118

[9] Guerrero-Preston R, Herbstman J, Goldman, L. (2011) Epigenomic biomonitors: global DNA hypomethylation as a biodosimeter of life-long environmental exposures. Epigenomics, 3(1):1-5. PMID: 22126146

[10] LeBron C, Pal  P, Brait M, Dasgupta S, Guerrero-Preston R, Looijenga L HJ, Netto G, Sidransky D, Hoque M. (2011) Genome-wide Analysis of Genetic Alterations in Seminoma using High Resolution Single Nucleotide Polymorphism (SNP) Arrays. Genomics. (6):341-9. PMID: 21376111

[11] Guerrero-Preston R, Soudry E, Acero J, Orera M, Moreno-López L, Jaffe A, Berdasco M, Ili-Gangas C, Brebi-Mieville P, Fu Y, Engstrom C, Irizarry R, Esteller M, Westra W, Koch W, Califano J, Sidransky D. (2011) NID2 and HOXA9 promoter hypermethylation as biomarkers for prevention and early detection in oral cavity squamous cell carcinoma tissues and saliva. Cancer Prev Res 4(7):1061-72. PMID: 21558411

[12] Keidar M, Walk R, Shashurin A, Srinivasan P, Sandler A, Dasgupta S, Ravi R, Guerrero-Preston R, Trink B. (2011)  Cold plasma selectivity and the possibility of a paradigm shift in cancer therapy. Br J Cancer. 105(9):1295-301. PMID: 21979421

[13] Brebi-Mieville P, Ili-Gangas C, Leal-Rojas P, Soudry E, Perez J, Roa J, Sidransky D, Guerrero-Preston R. (2012) Clinical and public health research using methylated DNA immunoprecipitation (MeDIP): A comparison of commercially available kits to examine differential DNA methylation across the genome. Epigenetics, 7(1):106 -112. PMID: 22207357

[14] Brait M, Munari E, Lebron C, Noordhuis MG, Begum S, Michailidi C, Gonzalez-Roibon N, Maldonado L, Sen T, Guerrero-Preston R, Cope L, Parrella P, Fazio VM, Ha PK, Netto GJ, Sidransky D, Hoque MO. (2013) Genome-wide methylation profiling and the PI3K-AKT pathway analysis associated with smoking in urothelial cell carcinoma. Cell Cycle. Feb 22;12(7). PMID: 23435205

[15] Brebi-Mieville P, Maldonado, L, Noorhuis MG, Ili-Gangas C, Leal-Rojas P, Garcia P, Brait M, Michailidi C, Perez J, Soudry E, Tapia O, Guzman P, Muñoz S, Van Neste L, Van Criekinge W, Irizarry R, Sidransky D, Roa JC, Guerrero-Preston R. (in-press) Genome-wide methylation profiling reveals Zinc finger protein 516 (ZNF516) and FK-506-binding protein 6 (FKBP6) promoters frequently methylated in cervical neoplasia, associated with HPV status and ethnicity in a Chilean population. Epigenetics.


[1] Esteller M, Guerrero-Preston R, Fraga M, Ballestar E. (2007). Epigenetic Biomarkers for Early Detection, Therapeutic Effectiveness, and Relapse Monitoring of Cancer. PCT/US2008/004736.

[2] Guerrero-Preston R, Brebi-Mieville P, Sidransky D  (2010). Hypermethylation Biomarkers for Detection of Cervical Cancer. PCT/US11/28045.

[3] Guerrero-Preston R, Soudry E, Sidransky D. (2010). Hypermethylation Biomarkers for Detection of Head and Neck Squamous Cell Cancer. PCT/US11/28011

[4] Guerrero-Preston R, Sidransky D. (2012). Global DNA hypomethylation and biomarkers of clinical indications in cancer. US Patent Application No: 2013/0035,247

[5] Guerrero-Preston R, Sidransky D. (2012) DNA hypermethylation of promoters of target genes and clinical diagnosis of HPV related disease. PCT/US2013/027897

[6] Guerrero-Preston R, Jedlicka A, Sidransky D. (2013) Quantification of Trans Renal HPV DNA in Urine Using a Dual Sequence-capture Approach.  Provisional Patent Application 61/771,462 

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