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Inside Tract - Enterohemorrhagic E. coli at work
Inside Tract Summer 2011
Enterohemorrhagic E. coli at work
Date: June 1, 2011
From her laboratory, Olga Kovbasnjuk has contributed this EM micrograph of an otherwise normal, healthy enterocyte infected with the O157:H7 strain of E. coli. Samples of the bacteria were collected from patients during an outbreak in Canada in 2000, in which people died from drinking contaminated water. In the lab, the pathogens were then introduced into enterocyte cultures.
This photo, for the most part, shows the normal apical brush border. The “elephant in the room,” however, is the large, infection-prompted “bleb” of actin, prior to its being reintegrated into the cell. Note the bacilli, which Kovbasnjuk says are excluded from being drawn into the enterocyte during the remodeling. The large opening in the actin bleb “most certainly” would give entry to the Shiga toxin already excreted by this strain of E. coli into the intestinal lumen.