Biopsy samples should remain refrigerated in fixative solution no longer than 24 hours. They must then be rinsed with buffer solution and placed in cryoprotectant solution as follows:
- Gently decant the fixative solution from the specimen tubes.
- The biopsy should remain in the tube
- In the event the biopsy tissue is poured out, the sample should be gently picked up with forceps and placed back into the tube.
- The tissue plug is then briefly washed with the phosphate buffer provided in your kit.
- Fill the tube containing the biopsy with the phosphate buffer solution and, after a few seconds, decant the buffer solution off. The biopsy should remain in the tube.
- Repeat rinse procedure one more time.
- Next, fill the tube completely with the cryoprotectant solution.
- Screw the cap on tightly taking care the sample is not caught between the tube and the cap.
- Place a strip of parafilm over the cap.
- Once in cryoprotectant, the biopsy should be kept refrigerated at 4 degrees C overnight. If shipping biopsy samples within 2 weeks, samples may be kept in the refrigerator. Samples MUST be refrigerated overnight in cryoprotectant before they are ever frozen for extended storage. If you will be batch-shipping samples at a later date, store them in a -20 degree C freezer. When ready to ship, use the refrigerated shipper provided to your site.
- Please record the time the biopsy was placed in cryoprotectant on the requisition form.
Watch the video of the biopsy.