(We are currently re-structuring this site and apologize for any errors you encounter. We are working to correct them as soon as possible. Thanks for your patience!)
|Home Page||General information on the A.B.Mass Spectrometry/Proteomics Facility|
|Reservations||Sign-up pages for equipment use|
|Equipment & Services||Mass spectrometers/ accessory equipment/ services|
|User Fees||User fees and cost estimates for projects|
|Budgeting!||Budget for mass spectrometry in your grants!|
|Courses & Workshops||Schedules for elective courses and workshops|
|Contacting Staff||Facility staff|
Mission: The mission of the Hopkins A.B.Mass Spectrometry /Proteomic Facility is to accelerate discovery by giving Hopkins investigators access to cutting edge technologies in mass spectrometry and proteomics. Mass spectrometry and Proteomics are rapidly advancing and requires considerable technical expertise and expensive equipment. To keep Hopkins scientists competitive, new technologies will be incorporated into the facility as soon as possible to meet your needs. The facility is open to all Hopkins investigators on a fee-for-service basis. User fees will be adjusted depending on demand and to keep costs competitive.
Location: The Hopkins A.B.Mass Spectrometry /Proteomic Facility is located in 601 Physiology Bldg and 503 Hunterian Bldg of the Basic Sciences Complex.
Equipment: The Mass Spectrometry Facility currently has three mass spectrometers, a Voyager DE-STR (Applied Biosystems), QSTAR/Pulsar (Applied Biosystems/MDS Sciex) and LCQ-DecaXP. The Voyager is a MALDI-TOF (matrix assisted laser desorption ionization with a time-of-flight analyzer) workstation with delayed extraction technology. It has a mass range of up to 500,000 Da for singly charged ions. The QSTAR is an electrospray ionization mass spectrometer with a TOF analyzer, and can detect masses up to 12,000 Da for singly charged ions. The LCQ also is an electrospray ionization mass spectrometer with an ion trap analyzer with a mass range of up to 4000 Da for singly charged ions. All instruments have high sensitivity (low fmol for peptides) and high resolution. The two high pressure liquid chromatography (HPLC) systems (Surveyor HPLC system from Thermo Finnigan and Ultimate/Switchos/Famos HPLC system from LC Packings/Dionex) are interfaced with the electrospray ionization mass spectrometers for microflow or nanoflow applications (high sensitivity) and on-line fragmentation, sequencing and identification of peptides. The facility also has a ProteomeX Workstation (Thermo Finnigan), a fully integrated multi-dimensional chromatography HPLC system interfaced with a LCQ ion trap mass spectrometer, and two dimensional (2D) gel electrophoresis equipment, gel spot handling robotics, gel imaging scanners (Amersham Biosciences).
Services: Our facility is equipped to analyze by mass spectrometry proteins, peptides, oligonucleotides, lipids, carbohydrates and small molecules from samples in solution or separated by chromatography or electrophoresis. In addition to conventional electrophoresis and chromatography fractionation, the facility will offer differential analysis of protein samples by Differential Imaging Gel Electrophoresis (DIGETM, Amersham Biosciences) analysis and Isotope-Coded Affinity Tag (ICATTM, Applied Biosystems) analysis.
The A.B Mass Spectrometry Facility is supported by The Johns Hopkins Institute
for Cell Engineering, The Johns Hopkins Fund for Medical Discovery , program
project grants and contracts, and user fees.
The Mass Spectometry/Proteomics Facility is now able to assist Hopkins investigators in their mass spectromety and proteomic projects. For questions on how to apply mass spectrometry/proteomics to your research, please call or email the director, Dr. Robert Cole, at 410-614-6968 (firstname.lastname@example.org).