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Urine for Bacterial, Fungal, AFB, and Viral Cultures

Specimen Guidelines

Urine for Bacterial, AFB, Fungal, Parasites and Viral Cultures

Instructions for female patients to collect midstream urine for bacterial culture:

  1. Remove undergarments.
  2. Wash hands thoroughly with soap and water, rinse them, and dry them on a disposable paper towel or shake off excess water.
  3. Spread labia, with one hand, and keep them continuously apart.
  4. Take the open sterile cup in the other hand without touching the rim or inner surface of the cup or lid.
  5. Void 20 to 25 ml into the toilet and catch a portion of the rest of the urine in the container without stopping the stream. Do not touch the legs, vulva, or clothing with the cup.
  6. Place the lid on the cup securely.

Instructions for male patients to collect midstream urine for bacterial culture:

  1. Wash hands.
  2. Retract the foreskin completely.
  3. Void 20 to 25 ml into the toilet and catch a portion of the remaining urine in the cup without stopping the stream. Do not touch the cup with the penis.
  4. Place the lid on the cup securely.

First void urine for nucleic acid amplification tests (Chlamydia/ Gonorrhea).

  1. Patient must not have urinated during the previous two hours.
  2. Collect the first 10 to 30 ml of the urine stream in a clean, empty plastic cup.
  3. Place the lid on the cup.
  4. Transport urine refrigerated in test-specific transport media.

Suprapubic aspiration:

  1. This is not a routine technique and is best performed by an experienced individual. Descriptions of the method are readily available in the literature.
  2. Faculty approval required for anaerobic culture, call 5-5077. Specimen should be submitted in an anaerobic environment if an anaerobic culture is approved.

Indwelling catheter urine:

  1. Do not collect urine from the drainage bag because growth of bacteria outside the catheter may have occurred at this site.
  2. Clean the catheter with an alcohol pad.
  3. Use a sterile needle and syringe to puncture the tubing. Aspirate the urine directly from the tubing.
  4. Transfer the urine to a sterile specimen container.
  5. Urine catheter tip cultures are not acceptable.

Specimen handling:

  1. Label the container immediately.

Notes:
a. Urine for CMV culture must be received within 1 hour of collection.
b. Minimum urine volume for AFB culture is 40 ml.

 

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