Molecular Microbiology Lab Johns Hopkins Hospital Medical Microbiology Division Part A: Viruses

Test

Test Code

Specimen types*

Vol

requested

Analytical sensitivity^†

Cut off: must receive by

EBV Quantitative (viral load)

7927

Whole blood (at least two 3 ml purple top tubes)

2 x 3 ml

(more if low WBC)

50 copies, (measure 1.7-7 log₁₀ copies/ml)

8 AM
(Tues & Thurs)

EBV Qualitative

7122

CSF

1.0 ml total if multiple tests ordered

^‡ Exception for vesicle:
swab fluid & send in 2 ml viral transport medium (VTM)

6-60 copies

9 AM 
(Mon, Wed, Fri)

HSV 1+2

7135

CSF;
Vitreous humor

10 copies

VZV

7131

CSF;
Vitreous humor;
Vesicle fluid (VTM ok^†);
Retinal biopsy

10 copies

JC

7901

CSF

100 copies

JC Quantitative
(viral load)

7305

400 copies/ml, measure 2.6 to 7.5 log10 copies/ml

9 AM
Thurs

CMV

7130

CSF;
Vitreous humor;
Amniotic fluid

20-200 copies

9 AM
(Mon, Wed, Fri)

CMV Quantitative (viral load)

7929

Plasma - use PPT tubes!!
Separate & freeze within 4 hr of collection!!
PPT = plasma preparation tube

1.0 ml

600 copies/ml,

measure 2.8-4.7 log₁₀ copies/ml

Anytime (assay Mon-Fri)

CMV Genotype

7921

Whole blood (purple);
Culture isolates;
BAL;
Fresh tissue

Fluids:0.5 ml

Tissue:40mg

unknown

Call lab 5-2642 for more info

Enteroviruses

7113

CSF;
Throat swabs in VTM;
Stool (or rectal swabs in VTM);
Plasma (purple)

1.0 ml

0.1 pfu/ml

9 AM
(Mon, Wed, Fri)

Parvovirus B19

7127

Plasma (purple);
Bone marrow (purple);
Amniotic fluid

1.0 ml

100 copies

9 AM
(Mon, Wed, Fri) 

Quant BK

7951

Plasma

1.0 ml

200 copies

9 AM Wed

BK

7129

Urine

1.0 ml total for one or both viruses

10 copies

9 AM Wed

Adenoviruses

7109

Urine;
Plasma (purple)

1 copy

HPV high risk

(Hybrid Capture)

7919

post-Autocyte Pap-smear preps;
Cervical samples (Digene devices only)

Requests must go through Cytopathology !!!

7:30 AM Thurs

Test

Code

Specimen: plasma*

Vol

Analytical sensitivity^†

Cut off: must receive by

HBV DNA Quantitative

6046

Use PPT tubes:
(Separate & freeze within 4 hr of collection!!)

PATH 6 Req: HBVQuant

HCV Quant, Gt

1.0 ml

200 copies/ml, measure 2.3 to >9 log₁₀ copies/ml

7:30 AM Tues

HCV RNA Quantitative

2915

0.5 ml

1500 IU/ml, measure 3.2-5.8 log₁₀ IU/ml

7:30 AM
(Tues, Wed, Thurs) 

 HCV Genotype

7195

0.5 ml

? 11,000 IU/ml

7:30 AM Mon