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Microscope Facility News & Equipment Status

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2014-07-25 (Zeiss LSM 780 FCS): 780/FCS database corrupted; restored with old configs

The database for the Zeiss 780 FCS was corrupted today (7/25) so it had to be replaced with an older version from 7/3/2014. This means that some configurations will NOT work. I did not have time to test all of the configurations on the system, but if your configuration will not work, you will get an error when you try to load the configuration. If you are not able to load your configuration, please use one of the MicFac standard configurations and set up your parameters. You can then Save a NEW configuration. Please DO NOT save over the MicFac Configuration. Please contact the staff or email microscopy@jhmi.edu if you have any questions or need more information. My apologies for any inconvenience this may cause. Thank you, Barbara Barbara J. Smith EM Specialist JHU SOM Microscope Facility Physiology G04 725 N. Wolfe Street Baltimore, MD 21205 410-614-6890 410-614-2086

2014-07-25 (Zeiss LSM 780 FCS): Zeiss 780 FCS: Database Corrupt

The database for the Zeiss 780 FCS was corrupted today so had to be replaced with an older version from July 3 of 2014. This means that some configurations will NOT work. I did not have time to test all of the configurations on the system, but if your configuration will not work, you will get an error when you try to load the configuration. If you are not able to load your configuration, please use one of the MicFac standard configurations and set up your parameters. You can then Save a NEW configuration. Please DO NOT save over the MicFac Configuration. Please contact the staff or email microscopy@jhmi.edu if you have any questions or need more information.

2014-07-23 (Nikon TIRF): Nikon TIRF back online, laser realigned

Our Nikon service tech was able to come out and check on the system this morning. We were able to fix the system and have realigned the laser. The system is back online and operational.

The service tech did note that when the TIRF angle is set to the limit of its travel, this situation is more likely to occur when it is moved out of the extreme. Please be sure you don’t set your angle at lower than 500 or higher than 6500.

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Hi TIRF users,

This afternoon an issue popped up with the TIRF motor. It appears to be stuck at its limit (0.00) and is unable to move because of a suspected safety mechanism. This means that the TIRF laser system is not usable until further notice. If you are using the mercury bulb for your imaging then the system should work perfectly fine.

We have contacted Nikon and they are sending us a trouble shooting sheet that we will try early next week. If that doesn’t work then they will need to come out to work on the system. The earliest that will happen is Wednesday.

We will email early next week with an update on estimated down time.

2014-07-17 (Zeiss LSM 780 FCS): new 780 shut down procedures

Over the past few weeks we have noticed a Z-drift issue on the 780. Through testing through us and Zeiss we have found that there seems to be something in the room that is causing this instability (most likely air flow over the stage). We found that the system needs to be on for at least an hour before the Z-drift stabilizes (2 hours seems better). For this reason we are changing the shut down procedure for the system.
From now on if someone is signed up after you (even a number of hours after you) leave the system ON. You can exit the software but please leave all of the microscope components on.
If you have any questions please don’t hesitate to ask or email microscopy@jhmi.edu.

2014-07-03 (Leica SDC w/ microinjector): Coolsnap Camera removed from Leica SDC

The Coolsnap camera that was used for phase contrast imaging on the Leica SDC system has been removed and placed on a different microscope. The operation of the microscope is unchanged. If you would like to use this camera on the Leica SDC or have any questions about the removal, please contact us at microscopy@jhmi.edu. Thank You

2014-07-01 (Zeiss LSM 510-meta #2): Zeiss 510 Meta2: New 405nm Laser

The Zeiss service engineer replaced the 405 nm laser today. Users may need to DECREASE the laser power/master gain when imaging to decrease the risk of bleaching/damaging the sample. We apologize for any inconvenience and thank you for your patience. Please see a member of the staff if you have any questions or need more information.

2014-06-06 (Leica Cryostat): Scheduled Cryostat Maintenance

The Cryostat will be down beginning Friday (6/6/14) afternoon until Monday (6/10/14) at noon. The Cryostat is being defrosted and cleaned over the weekend. Sorry for any inconveniences.

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