Perkin Elmer UltraVIEW
Spinning Disk Confocal Microscopes

Specifications

  • PerkinElmer UltraView LCI (Live Cell Imaging) System
  • Spinning Nipkow disk with microlenses
  • Kr/Ar laser with lines at 488, 568 and 647 nm
  • Zeiss Axiovert 200 and Nikon Eclipse 200 platforms
  • LSI cooled 12-bit CCD camera
  • Dell Optiplex 400 computer, UltraView software
  • Volocity software for 3D viewing

  • Applications

  • Optical sections or image stack with high contrast and definition
  • Best for moderate and bright fluorescence
  • Low rate photobleaching/ extended cell viability
  • Minute details of points and edges
  • Simple-to-use, easy software
  • Movie loops of 3D displays exportable to QuickTime or Volocity
  • Quantitative 3D analysis
  • Quantitative colocalization analysis


  • Examples

    Protein in nuclei of Drosophila embryo


    Pole cells in Drosophila germarium


    http://lifesciences.perkinelmer.com/livecellimaging/intro.asp