Single-point, laser scanning confocal microscope

Specifications

·        Zeiss Axiovert 200  microscope with  510-Meta confocal module

·        Bioptechs 37°C environmental control also available.

·        NA 0.55 condenser (dry only) with DIC for 40X, 63X and 100X lenses

·        Objectives

10X Planapo, 20X Planapo (dry, no DIC)

40 X PlanNeofluar oil, with DIC

63X Planapo oil,  with DIC

100X Planapo oil, with DIC

·        7 laser lines available

405 (diode laser),

458, 477, 488, 514 (argon laser)

542 (green HeNe)

633 (red HeNe)

·        HBO 100W illuminator and Fluorarc power supply for fluorescence

·        Fluorescence filter sets

#47 for CFP,# 02 for DAPI, #10 for FITC, #15 For TRITC

·        Axiovision software, modules for Physiology and Time Series

·        Axiovision software on a remote workstation

·        LSM Image Browser software available for desktop computers in your lab

Applications

·        Single confocal images or image stacks for 3D viewing

·        FRAP with user-defined photobleach marks

·        CFP/YFP FRET

·        Photoactivation at 405 nm of caged dyes and other molecules

·        Colocalization of different  fluorescent signals

·        Speed and versability

Meta "fingerprint" system separates very closely spaced fluorochromes.  Example:  DPPIV-GFP (Golgi, green) and Albumin-Alexa 488 (ER-Golgi, red)