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Spring 2011 Events

  • May 9-May 19, 2011 Leica CW-STED Super-resolution Demo (2nd visit)
  • May 25, 2011, 2p-3p: Seminar on using high-pressure freezing and freeze-substitution

May 9-19, Leica CW-STED Super-Resolution Demo

The Microscope Facility is pleased to host a second demo of Leica's CW-STED for two-color fluorescence. It's the first super-resolution technique that balances high-speed (5-25 fps) with high resolution (70nm). Normal confocal microscopy has only ~250nm resolution, so CW-STED is ~4x better!

New developments since the Leica demo a year ago:

  • Better, brighter dyes and color combinations for 2nd color for two-color super-resolution.
  • Lower-noise and higher signal-to-noise (~5 times better!) detectors
    {"HyD" hybrid detectors; On 4/26/11, confirmed to be at demo}
  • On 4/26/11, Room confirmed: PCTB 207

 

May 25, 2011, 2p-3p: Seminar on using high-pressure freezing and freeze-substitution

Kim Rensing (Leica Microsystem) will introduce the Microscope Facility's newest acquisitions: HPM100 (high-pressure freezer) and AFS2 (automated freeze-substitution).  Becoming the gold-standard of specimen preparation, these techniques preserve cellular ultrastructure with great fidelity.  With <1 min delay, cells imaged by fluorescence/light can be frozen and later imaged by TEM using the CLEM imaging kit (Correlative Light-EM).

Location: Bodian Conference Center, 1830 E Monument St, Suite 2-200


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