Multiphoton, Second-harmonic generation and Confocal Microscope
Zeiss LSM 710NLO-Meta

Model Information

• Zeiss AxioExaminer with 710NLO-Meta multiphoton and confocal module with spectral detection
• Coherent Chameleon VisionII (TiSapph) Laser with broadest spectral range and an integrated compensator for group-velocity dispersion (deepest penetration)

 

Specifications

• Zeiss AxioExaminer (upright) microscope with 710NLO-Meta confocal module
• fully automated microscope with LCD remote control
• NA 0.55 condenser (dry only) with DIC for 20x, 25x, 40x and 63x lenses
• Objectives
• 10x/0.3 EC-PlanNeofluar (dry)
• 20x/1.0 W-PlanApo, water
• 25x/0.8 LD LCI PlanApo, multi-immersion, optimized for multiphoton, astounding 0.59 mm penetration
• 40x/1.1W LD C-Apo, water, optimized for confocal
• 63x/1.2W C-Apo, water, optimized for confocal
• 5 laser lines (not multiphoton) available
• 458, 488, 514 (argon laser)
• 543 (green HeNe)
• 633 (red HeNe)
• Chameleon Vision II (automated pre-compensation; extremely fast scanning 40 nm/s)
• 680 - 1080 nm (ideal for 2-photon excitation of commonly used fluorophores and fluorescent proteins)
• EXFO X-Cite 120XL light-guide coupled metal-halide bulb for wide-field fluorescence
• Fluorescence filter sets
• #49 for DAPI, #38 for GFP, #43 For CY3
• Three spectral detection channels (formerly "Meta" and "fingerprint" capabilities)
  • arbitrary bandpass controlled by software
  • simple menu selection for specific dye combinations)
• Three non-descanned detectors (for multiphoton): two epi-fluorescence and one transmission
  • filter sets for red/green (Filter Set 2u), or for CFP/YFP (Filter Set 7u)
• Zen software, modules for Physiology, FRET, FRAP, and MultiTime

Applications

• Single confocal/multiphoton images or image stacks for 3D viewing
• FRAP with user-defined photobleach marks
• CFP/YFP FRET
• Photoactivation of caged dyes and other molecules
• Colocalization of different fluorescent signals
• Speed and versatility