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Zeiss LSM700
Single-point, laser scanning confocal microscope

Model Information

  • Zeiss AxioObserver with LSM700 confocal module
  • Cite in your manuscripts: NIH Grant S10 OD016374
    (Institute:Office of the Director [OD], Office of Research Infrastructure Programs [ORIP])
    Installed: Dec 11, 2013

Specifications:

  • Zeiss AxioObserver inverted microscope with LSM700 confocal module
  • Portable 37°C heater for 35mm dishes also available
  • UPDATE REST LATER
  • NA 0.55 condenser (dry only) with DIC for 40X, 63X and 100X lenses
  • ·Objectives:
    • 10X Planapo, 20X Planapo (dry, no DIC)
    • 40 X PlanNeofluar oil, with DIC
    • 63X Planapo oil,  with DIC
    • 100X Planapo oil, with DIC
  • 7 laser lines available:
    • 405 (diode laser),
    • 458, 477, 488, 514 (argon laser)
    • 542 (green HeNe, Meta1 only)
    • 561 (solid-state, Meta2 only)
    • 633 (red HeNe)
  • HBO 100W mercury (Meta1) or HXP 100W metal-halide (Meta2) illuminator for wide-field fluorescence
  • Fluorescence filter sets (wide-field; non-confocal):
    • #47 for CFP,# 02 for DAPI, #10 for FITC, #15 For TRITC
  • Latest Zen software to control microscope and confocal
  • Zen software on a separate workstation for image analysis.
  • Download Zen LE software available for desktop computers in your lab
    • Analysis with Volocity, Imaris or ImageJ on our analysis workstations
    • Use LOCI BioFormats to import images into ImageJ

Applications

  • Single confocal images or image stacks for 3D viewing
  • FRAP with user-defined photobleach marks
  • CFP/YFP FRET
  • Photoactivation at 405 nm of caged dyes and other molecules
  • Colocalization of different  fluorescent signals
  • Speed and versatility
  • Meta "fingerprint" system separates very closely spaced fluorochromes.  Example:  DPPIV-GFP (Golgi, green) and Albumin-Alexa 488 (ER-Golgi, red)

510 example


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