Zeiss LSM510-Meta
Single-point, laser scanning confocal microscope

Model Information

• Zeiss Axiovert 200 with 510-Meta confocal module

Specifications:

• Zeiss Axiovert 200 inverted microscope with LSM510-Meta confocal module
• 37°C heater for 35mm dishes also available.
• NA 0.55 condenser (dry only) with DIC for 40X, 63X and 100X lenses
• ·Objectives:
  • 10X Planapo, 20X Planapo (dry, no DIC)
  • 40 X PlanNeofluar oil, with DIC
  • 63X Planapo oil,  with DIC
  • 100X Planapo oil, with DIC
• 7 laser lines available:
  • 405 (diode laser),
  • 458, 477, 488, 514 (argon laser)
  • 542 (green HeNe, Meta1 only)
  • 561 (solid-state, Meta2 only)
  • 633 (red HeNe)
• HBO 100W mercury (Meta1) or HXP 100W metal-halide (Meta2) illuminator for wide-field fluorescence
• Fluorescence filter sets (wide-field; non-confocal):
  • #47 for CFP,# 02 for DAPI, #10 for FITC, #15 For TRITC
• Latest Zen software to control microscope and confocal
• Zen software on a separate workstation for image analysis.
• Download Zen LE software available for desktop computers in your lab
  • Analysis with Volocity, Imaris or ImageJ on our analysis workstations
  • Use LOCI BioFormats to import images into ImageJ

Applications

• Single confocal images or image stacks for 3D viewing
• FRAP with user-defined photobleach marks
• CFP/YFP FRET
• Photoactivation at 405 nm of caged dyes and other molecules
• Colocalization of different  fluorescent signals
• Speed and versatility
• Meta "fingerprint" system separates very closely spaced fluorochromes.  Example:  DPPIV-GFP (Golgi, green) and Albumin-Alexa 488 (ER-Golgi, red)