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Zeiss LSM510-Meta
Single-point, laser scanning confocal microscope

  • Meta1 has been removed (dismantled) January 20, 2014, and is no longer available.
  • , call 410-614-6890, or visit the to request to be re-trained for Meta1's replacement, the LSM700.
  • Meta2 (stronger red-fluor laser) will stay.

Model Information

  • Zeiss Axiovert 200 with 510-Meta confocal module

Specifications:

  • Zeiss Axiovert 200 inverted microscope with LSM510-Meta confocal module
  • 37°C heater for 35mm dishes or chamber slides also available.
  • NA 0.55 condenser (dry only) with DIC for 40X, 63X and 100X lenses
  • Objectives:
    • 10X/0.30 PlanNeofluar (dry, no DIC)
    • 20X/0.80 PlanApo (dry, no DIC)
    • 40 X PlanNeofluar oil, with DIC
    • 63X PlanApo oil,  with DIC
    • 100X PlanApo oil, with DIC
  • 7 laser lines available:
    • 405 (diode laser),
    • 458, 477, 488, 514 (argon laser)
    • 561 (solid-state)
    • 633 (red HeNe)
  • HXP 100W metal-halide illuminator for wide-field fluorescence
  • Fluorescence filter sets (wide-field; non-confocal):
    • #47 for CFP,# 02 for DAPI, #10 for FITC, #15 For TRITC
  • Latest Zen software to control microscope and confocal
  • Zen software on a separate workstation for image analysis.
  • Download Zen LE software available for desktop computers in your lab
    • Analysis with Volocity, Imaris or ImageJ on our analysis workstations
    • Use LOCI BioFormats to import images into ImageJ

Applications

  • Single confocal images or image stacks for 3D viewing
  • FRAP with user-defined photobleach marks
  • CFP/YFP FRET
  • Photoactivation at 405 nm of caged dyes and other molecules
  • Colocalization of different  fluorescent signals
  • Speed and versatility
  • Meta "fingerprint" system separates very closely spaced fluorochromes.  Example:  DPPIV-GFP (Golgi, green) and Albumin-Alexa 488 (ER-Golgi, red)

510 example


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