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Motorized upright fluorescence microscope
Model Information
- Olympus BX61 with Roper/Photometrics Coolsnap HQ (extended spectral sensitivity)
Specifications
- Olympus BX61 motorized upright microscope with fluorescence and phase contrast optics for immunofluorescence imaging
- Olympus motorized z-axis stage and external filter wheels work with multiwavelengthdichroics for accurate in color registration and imaging speed for FRET
- Roper Photometrics CoolSnap HQ CCD camera with 70% quantum efficiency for highest resolution imaging
- Semrock fluorescence filters with the new hard surface coatings that are extremely high contrast and virtually eliminate bleedthrough
| Fluorescence Cube | Mfg ID | |
|---|---|---|
| Installed | DAPI | Semrock 5060B |
| FITC | Semrock 3540B | |
| TRITC | Semrock 4040A | |
| Cy5 | Semrock 4040A | |
| Quad: DAPI, FITC, rhodamine, Cy5 | Semrock | |
| Tri: DAPI, FITC, Texas red | Semrock | |
| Available | GFP | Semrock 3035A |
| CFP | Semrock 2432A | |
| YFP | Semrock 2427A | |
| dsRed | Semrock 4040B | |
| CFP/YFP dual | Chroma 86002 v1 | |
| eGFP/dsRed dual | Chroma 86007 |
- Olympus UIS2 objectives: 4X/ 0.16, 10X/ 0.3, 40X/ 0.75, 60X/ 1.40 oil, and 100X/ 1.3 oil give the brightest, flattest, high-contrast images of any microscope objective and are achromatic to close tolerances over an extraordinary range—350 to 1000 nm. Phase contrast for 10, 40, 60, and 100X lenses.