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Nikon Eclipse Ti-E
for
Total Internal Reflection Fluorescence (TIRF) Microscopy
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Applications
- Widefield or TIRF fluorescence imaging of membrane proteins, receptor proteins, signalling molecules, cortical cytoskeleton proteins
Special Capabilites
- TIRF illumination exciting molecules within 100 nm of the coverslip surface
- integrated Perfect Focus System to address focus drift with time-lapse imaging
- Photometrics Evolve EMCCD camera: Absolute sensitivity calibration (photon-counting), single-molecule detection with Quant-view and Background Event Reduction Technology (BERT)
- Simultaneous acquisition of two spatially identical but spectrally distinct images using Photometrics Dual-View
System Information
| Microscope Model: | Nikon Eclipse Ti-E |
| Camera Model: | Photometrics Evolve 512 EMCCD |
| Imaging Software: | Nikon NIS-Elements v3.22.00 |
| Accessories available: | Warner Instruments objective and stage heaters for live-cell imaging |
Microscope Specifications
Objectives |
|
| Installed | 20x/0.45 S Plan Fluor (dry) |
| 40x/0.60Ph2 S Plan Fluor (dry) | |
| 100x/1.49 Apo TIRF (oil) | |
| Available | ? |
Filter sets |
|
| Eyepiece View | CFP |
| YFP | |
| Camera View (both TIRF & Widefield, filter-wheel on emission) |
CFP, YFP, CFP-YFP FRET |
| GFP, RFP, GFP-RFP FRET | |
| Dual-View | CFP/YFP |
| GFP/RFP | |
Laser Lines |
|
| CFP | 445nm (40mW Diode laser) |
| not standard cube | 457nm (Argon laser) |
| not standard cube | 477nm (Argon laser) |
| GFP | 488nm (Argon laser) |
| YFP | 514nm (Argon laser) |
| RFP | 561nm (100mW Diode laser) |