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Nikon Eclipse Ti-E
for Total Internal Reflection Fluorescence (TIRF) Microscopy

Applications

  • Widefield or TIRF fluorescence imaging of membrane proteins, receptor proteins, signalling molecules, cortical cytoskeleton proteins

Special Capabilites

  • TIRF illumination exciting molecules within 100 nm of the coverslip surface
  • integrated Perfect Focus System to address focus drift with time-lapse imaging
  • Photometrics Evolve EMCCD camera: Absolute sensitivity calibration (photon-counting), single-molecule detection with Quant-view and Background Event Reduction Technology (BERT)
  • Simultaneous acquisition of two spatially identical but spectrally distinct images using Photometrics Dual-View

System Information

Microscope Model: Nikon Eclipse Ti-E
Camera Model: Photometrics Evolve 512 EMCCD
Imaging Software: Nikon NIS-Elements v3.22.00
Accessories available: Warner Instruments objective and stage heaters for live-cell imaging

Microscope Specifications

Objectives
Installed 20x/0.45 S Plan Fluor (dry)
40x/0.60Ph2 S Plan Fluor (dry)
100x/1.49 Apo TIRF (oil)
Available ?
 
Filter sets
Eyepiece View CFP
YFP
Camera View
(both TIRF & Widefield, filter-wheel on emission)
CFP, YFP, CFP-YFP FRET
GFP, RFP, GFP-RFP FRET
Dual-View CFP/YFP
GFP/RFP
   
Laser Lines
CFP 445nm (40mW Diode laser)
not standard cube 457nm (Argon laser)
not standard cube 477nm (Argon laser)
GFP 488nm (Argon laser)
YFP 514nm (Argon laser)
RFP 561nm (100mW Diode laser)

 


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