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Zeiss/Eppendorf Microinjection System
Model Information
- Eppendorf #5171 micromanipulator with Femtojet transjector
- Typically mounted on Zeiss Axiovert 100 inverted microscope (manual microscope: phase or fluorescence only)
- Alternative: can mount microinjector system on Leica SDC: Leica DMI6000 fully automated inverted fluorescence microscope with spinning disk confocal
Specifications
- Choice of Zeiss Axiovert 100 inverted microscope base OR Leica SDC (fully automated inverted fluorescence microscope with spinning disk confocal)
- Zeiss system: 10X and 32X phase contrast objectives
- Quartz-halogen or mercury illumination
- Eppendorf #5171 micromanipulator and Femtojet transjector
- Ports for suction/transfer and pressure/injection
- Roper/Photometrics CoolSnapEZ CCD camera
Applications
- Microinjection of plasmids, antibodies, dyes into cells
- Nuclear injection to transfect cells
- Widefield fluorescence viewing
Examples
Stimulation of chemotaxis in Dictyostelium
Controlled manipulation of cell signaling in Dictyostelium