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Zeiss/Eppendorf Microinjection System

Model Information

  • Eppendorf #5171 micromanipulator with Femtojet transjector
  • Typically mounted on Zeiss Axiovert 100 inverted microscope (manual microscope: phase or fluorescence only)
  • Alternative: can mount microinjector system on Leica SDC: Leica DMI6000 fully automated inverted fluorescence microscope with spinning disk confocal

microinjection station

Specifications

  • Choice of Zeiss Axiovert 100 inverted microscope base OR Leica SDC (fully automated inverted fluorescence microscope with spinning disk confocal)
  • Zeiss system: 10X and 32X phase contrast objectives
  • Quartz-halogen or mercury illumination
  • Eppendorf #5171 micromanipulator and Femtojet transjector
  • Ports for suction/transfer and pressure/injection
  • Roper/Photometrics CoolSnapEZ CCD camera

Applications

  • Microinjection of plasmids, antibodies, dyes into cells
  • Nuclear injection to transfect cells
  • Widefield fluorescence viewing

Examples


Stimulation of chemotaxis in Dictyostelium


Controlled manipulation of cell signaling in Dictyostelium


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