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Micropipette/microaspiration (cell mechanics)
Total-internal Reflection Fluorescence Microscope (TIRF)

Model Information

  • Olympus IX81 motorized microscope

Specifications

  • Olympus IX 81 motorized inverted  microscope
  • Olympus TIRF illuminator with fiber optic input
  • Bioptechs 37°C environmental control also available.
  • Condenser, 0.55 NA
  • Objectives:
    • 10X UplanFl, 0.3 NA, PC, dry
    • 40X UplanFl, 1.3 NA, oil, DIC-capable
    • 60X Planapo, 1.45 NA, oil (TIRF lens)
  • Argon laser line at 488 nm for TIRF
  • HBO 100W illuminator for widefield  fluorescence
  • Fluorescence filter sets: DAPI, FITC, TRITC, GFP
  • MetaMorph  software, UCI
  • Photometrics CoolSnap HQ CCD camera
  • Equipment for microaspiration

Applications

  • Widefield or TIRF fluorescence images of membrane proteins, receptor proteins, signalling molecules, cortical cytoskeleton proteins; TIRF excites fluorochromes within 100 nm of the coverslip surface
  • Micromanipulators/micropipettes for deforming cell surfaces by quantitative micro-aspiration, allowing measurements of cell cortical stiffness.
  • This microscope is made available to members of the microscope facility by Dr. Doug Robinson in the Department of Cell Biology

Example:

Myosin thick filaments in the cortex of live Dictyostelium cells.


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