Leica/3i Spinning Disk Confocal
Modular & customizable optics/microinjection

Model Information

• 3i Marianis/Yokogawa Spinning Disk Confocal
• Leica DMI6000 fully motorized inverted microscope (Marzhauser xy stage)
• Metal-halide illuminator with fiber-optic coupling (long-life 2000 hr/bulb)
• Extra illuminator and camera ports; fully modular and customizable (ask us!)

 

Specifications

• Yokogawa CSU10 spinning Nipkow disk with microlenses
• Leica DMI6000 fully motorized platforms
• Kr/Ar laser with lines at 488, 568 and 647 nm
• 3i Slidebook software for intuitive data acquisition
• Copy of software at workstation for 3D viewing & analysis (also Volocity software for 3D viewing and analysis)

Applications

• Optical sections or image stack with high contrast and definition
• Best for moderate and bright fluorescence
• Low rate photobleaching/ extended cell viability
• Minute details of points and edges
• Simple-to-use, easy software
• Movie loops of 3D displays exportable to QuickTime or Volocity
• Quantitative 3D analysis
• Quantitative colocalization analysis

Examples

Protein in nuclei of Drosophila embryo

Pole cells in Drosophila germarium