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High-pressure Freezer &
Automated Freeze-substitution System
Model Information
- Still to purchase
Specifications
- Still to purchase
- Freeze specimen under 20atm within milliseconds! Prevent formation of ice-crystals that damage cell membranes
- When frozen, crosslinking agents, particularly membrane-stabilizing reagents (e.g. osmium tetroxide), still work! Rates are slower, but preservation is excellent!
- Correlative light/fluorescence microscopy:
- Grow cells on special sapphire coverslips.
- Observe cells on light/fluorescence/confocal microscope (your choice!)
- After walking down the hall, freeze within 10s!
- After processing, compare light/fluorescence images with EM.
Applications
- Freezing whole Drosophila embryos
Examples