|
David L. Huso, D.V.M., Ph.D., Dip ACVP
Diplomate, American College of Veterinary Pathology
Associate Professor, Department of Molecular and Comparative Pathobiology
& The Sidney Kimmel Comprehensive Cancer Center
Background and Training
Dr. Huso received his veterinary degree from Iowa State University and entered private practice following graduation. He then completed a postdoctoral fellowship in comparative pathology at The Johns Hopkins University School of Medicine and achieved board certification from the American College of Veterinary Pathology. Concurrent with his pathology fellowship, he completed his Ph.D. training at Johns Hopkins focusing on molecular pathogenesis and mechanisms of disease. Following his training he received a faculty appoinment and started his own research
laboratory in Comparative Medicine at the Johns Hopkins Bayview Research Campus. He has moved his research laboratory back to the JHMI campus and is now Associate Professor in the Department of Molecular and Comparative Pathobiology at The Johns Hopkins University School of Medicine.
Current Roles
Dr. Huso mentors a number of individuals both inside and outside the department with research interests in the molecular pathogenesis of genetic diseases. He also is director of a graduate school class covering the basics of comparative histology and development of genetically engineered mice and other models. His research utilizes molecular techniques and mouse models to facilitate the movement of basic science discoveries to the clinic to benefit patients. In addition, he devotes a portion of his time to pathology training and diagnostic pathology for the research mouse research colonies at JHU.
Research
Dr. Huso’s laboratory uses the mouse as a genetic system in which to determine mammalian gene function and to elucidate the details of the molecular pathogenesis and treatment of human diseases. This system, along with basic molecular techniques, provides a platform for discovery of underlying disease mechanisms as well as rapid validation of noval targeted therapies using a rational approach based on molecular mechanisms of diseases. Accurate and predictive mouse models are developed and used extensively, not only in discovery and evaluation of efficacy of emerging therapeutic approaches, but also to determine the safety of new therapeutics. Dr. Huso’s research laboratory is made up of persons with diverse backgrounds including students, fellows, assistants and associates with backgrounds in veterinary and human medicine.
Relevant recent articles
Zhou YF, Bosch-Marce M, Okuyama H, Krishnamachary B, Kimura H, Zhang L, Huso DL, and Samenza GL. Spontaneous Transformation of Cultured Mouse Bone Marrow-Derived Stromal Cells. 2006. Cancer Res 66: 10849-10854
Habashi JP, Judge DP, Hom TM, Cohn RD, Loeys BL, Cooper TK, Myers L, Klein EC, Liu G, Calvi C, Podowski M, Neptune ER, Halushka MK, Bedja D, Gabrielson K, Rifkin DB, Carta L, Ramirez F, Huso DL, and Dietz HC. Losartan, an AT1 antagonist, prevents aortic aneurysm in a mouse model of Marfan Syndrome. Science. 2006. 312:117-121
Yadav A, Pati S, Nyugen A, Barabitskaja O, Mondal P, Anderson M, Gallo RC, Huso DL, Reid W. HIV-1 transgenic rat CD4+ T cells develop decreased CD28 responsiveness and suboptimal Lck tyrosine dephosphorylation following activation. 2006. Virology 353:357-365.
Nanda A, Karim B, Peng Z, Liu G, Qiu W, Gan C, Vogelstein B, St Croix B, Kinzler KW, and Huso DL. Tumor endothelial marker 1 (Tem1) functions in the growth and progression of abdominal tumors. Proc Natl Acad Sci U S A. 2006. 103:3351-3356.
Hao LY, Armanios M, Strong MA, Karim B, Feldser DM, Huso D, and Greider CW. Short telomeres, even in the presence of telomerase, limit tissue renewal capacity. Cell. 2005. 123:1121-1131.
Wang Y, Huso DL, Harrington J, Kellner J, Jeong DK, Turney J, and McNiece IK. Outgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture. Cytotherapy. 2005. 7:509-519.
Diaz LA Jr, Cheong I, Foss CA, Zhang X, Peters BA, Agrawal N, Bettegowda C, Karim B, Liu G, Khan K, Huang X, Kohli M, Dang LH, Hwang P, Vogelstein A, Garrett-Mayer E, Kobrin B, Pomper M, Zhou S, Kinzler KW, Vogelstein B, and Huso DL. Pharmacologic and toxicologic evaluation of C. novyi-NT spores. Toxicol Sci. 2005. 88:562-575.
Vaupel DB, Tella SR, Huso DL, Wagner VO 3rd, Mukhin AG, Chefer SI, Horti AG, London ED, Koren AO, and Kimes AS. Pharmacological and toxicological evaluation of 2-fluoro-3-(2(S)-azetidinylmethoxy)pyridine (2-F-A-85380), a ligand for imaging cerebral nicotinic acetylcholine receptors with positron emission tomography. J Pharmacol Exp Ther. 2005. 312:355-365.
Peterson NC, Servinsky MD, Christian A, Peng Z, Qiu W, Mann J, Dicello J, and Huso DL. Tamoxifen resistance and Her2/neu expression in an aged, irradiated rat breast carcinoma model. Carcinogenesis. 2005. 26:1542-1552.
Wang Y, Xiao R, Yang F, Karim BO, Iacovelli AJ, Cai J, Lerner CP, Richtsmeier JT, Leszl JM, Hill CA, Yu K, Ornitz DM, Elisseeff J, Huso DL, and Jabs EW. Abnormalities in cartilage and bone development in the Apert syndrome FGFR2(+/S252W) mouse. Development. 2005. 132:3537-3548.
Samuels Y, Diaz LA Jr, Schmidt-Kittler O, Cummins JM, Delong L, Cheong I, Rago C, Huso DL, Lengauer C, Kinzler KW, Vogelstein B, and Velculescu VE. Mutant PIK3CA promotes cell growth and invasion of human cancer cells. Cancer Cell. 2005. 7:561-573.
Germain-Lee EL, Schwindinger W, Crane JL, Zewdu R, Zweifel LS, Wand G, Huso DL, Saji M, Ringel MD, Levine MA. A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas gene. Endocrinology. 2005. 146:4697-4709.
Osthus RC, Karim B, Prescott JE, Smith BD, McDevitt M, Huso DL, Dang CV. The Myc target gene JPO1/CDCA7 is frequently overexpressed in human tumors and has limited transforming activity in vivo. Cancer Res. 2005. 65:5620-5627.
Dicello JF, Christian A, Cucinotta FA, Gridley DS, Kathirithamby R, Mann J, Markham AR, Moyers MF, Novak GR, Piantadosi S, Ricart-Arbona R, Simonson DM, Strandberg JD, Vazquez M, Williams JR, Zhang Y, Zhou H, Huso D. In vivo mammary tumourigenesis in the Sprague-Dawley rat and microdosimetric correlates. Phys Med Biol. 2004. 49:3817-3830.
