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- B.A., Boston University, University Professors Program, Trustee Scholars Program
- D.V.M., Purdue University School of Veterinary Medicine
- Ph.D., Johns Hopkins University School of Medicine
- Diplomate, American College of Laboratory Animal Medicine
Dr. Metcalf Pate’s research focuses on the role of platelets in the innate immune response to viral infection, and how modulating the response of platelets to infection alters the course of disease. Platelets are tiny anuclear cells that outnumber leukocytes in the peripheral blood more than one hundred to one. Platelets are known to participate in innate immunity through cytokine signaling and direct interactions with other cells, and the platelet has the potential to significantly influence disease outcomes. However, platelet immunology is still a relatively new discipline, and the downstream effects of platelet interactions with other immune cells have yet to be determined in the context of viral infection.
The well-characterized and consistent SIV-infected pigtailed macaque model of HIV infection provides an ideal animal model in which to explore the consequences of platelet-leukocyte aggregates during acute infection. Dr. Metcalf Pate has demonstrated that 80% of CD16+ monocytes are bound to platelets during acute SIV infection, while only 6% of this population are bound in mock-inoculated controls. CD16+ monocytes are known to play important roles in the pathogenesis of SIV infection as they are infected more frequently than CD16- monocytes and transmigrate through endothelium early in infection to establish inflammatory foci in organs such as the brain and lungs. Current research aims include further characterization of the platelet-monocyte interaction during acute viral infection with the goal of establishing methods of pharmacologically manipulating this association, and establishing how platelet binding to a monocyte influences the monocyte’s susceptibility to lentiviral infection and the monocyte’s interactions with endothelium.
Dr. Metcalf Pate is additionally interested in the effect of physiologic stress on platelet function, specifically on the platelet’s future immune response to infection, and in the development and optimization of novel in vitro systems that better model in vivo conditions. She welcomes collaborative inquiries from researchers with complementary interests.
Current Funded Projects
- “The Role of Platelet-Monocyte Interactions in SIV Infection”, NIH OD K01 OD018244, in collaboration with Dr. Chris Zink, Dr. Janice Clements, Dr. Lucio Gama and Dr. Wilbur Lam
- “A Mouse Model of the Role of Platelets in the Establishment of Latent Viral Reservoirs”, The Johns Hopkins NIMH Center for Novel Therapeutics for HIV-Associated Cognitive Disorders, in collaboration with Dr. Ravit Boger
- “Effect of Changes in Housing Environment on Platelet Activation in Macaques”, GLAS Foundation, in collaboration with Dr. Ken Witwer and Dr. Bob Adams
Awards and Recognition
- Dr. Metcalf Pate’s work was featured in a July 2013 Journal of Infectious Diseases Editorial.
- Speaker, 2013 Gordon Seminar on Cell Biology of Megakaryocytes and Platelets
- Young Investigator Travel Award, 2012 Conference on Retroviruses and Opportunistic Infections
- Poster Award, 2012 Molecular and Comparative Pathobiology Year in Review, Johns Hopkins University
Current Students and Visiting Scholars
- Jian (John) Li, MD, Visiting Scientist, PLA General Hospital, Beijing, China – John is interested in mechanisms underlying platelet activation and functional abnormalities in the context of anti-retroviral therapy.
- Meghan Vermillion, DVM, Postdoctoral Fellow, Johns Hopkins University School of Medicine - Meghan’s work focuses on the effect of social stress on platelet function in pigtailed macaques.
- Hayley Weidenbenner, Undergraduate Researcher, Johns Hopkins University School of Medicine
Current Research Staff
- Kevin Najarro, Research Technologist
- Liz Engle, Research Specialist
Former Students and Staff
- Claire Lyons, Undergraduate Researcher 2012 – 2013, Research Technologist 2013 - 2014, currently a veterinary student at Tufts University Cummings School of Veterinary Medicine.
- Catherine Cryer, Summer Veterinary Student Researcher 2014, currently a veterinary student at University of Pennsylvannia School of Veterinary Medicine.
- Hannah Schneider, Summer Undergraduate Researcher 2013, currently an undergraduate at Colorado State University.
Teaching and Service
Dr. Metcalf Pate is a veterinarian with Research Animal Resources, acts as an advisor within the Laboratory Animal Medicine training program and is a member of the Internal Advisory Committee for the T32 Postdoctoral Research Training Program for Veterinarians.
She provides instruction in the following Johns Hopkins University School of Medicine courses:
- LAM/PATH Integrated Problem Solving (ME680.702), Director and Lecturer
- Regulations that Govern Animal Research (ME:680.713), Director and Discussion Leader
- The Human Body (ME800.702), Lecturer
- Introduction to Research Ethics (School of Medicine Graduate Student Affairs), Lecturer
- Mouse Pathobiology and Phenotyping Course (ME680:712), Laboratory Instructor
- Clinical Conference in Laboratory Animal Medicine (ME680:710), Mentor
Dr. Metcalf Pate volunteers to coordinate food and beverage services for the Pan Mass Challenge start site in Sturbridge, MA every August.
Dr. Metcalf Pate enjoys sharing the excitement of scientific discovery with others! Local undergraduate and veterinary students with an interest in Dr. Metcalf Pate’s research are encouraged to inquire about available projects and research opportunities.
- Metcalf Pate KA, Mankowski JL.HIV and SIV associated thrombocytopenia: an expanding role for platelets in the pathogenesis of HIV. Drug Discov Today Dis Mech. 2011; 8(1-2): e25-e32. PMCID 22577463. Invited Review.
- Metcalf Pate KA, Lyons CE, Dorsey JL, Shirk EN, Queen SE, Adams RJ, Gama L, Morrell CN, Mankowski JL. Platelet activation and platelet-monocyte aggregate formation contribute to platelet decline during acute SIV infection in pigtailed macaques. J Infect Dis. 2013; 208(6):874-883. PMCID23852120
- Featured in Editorial by: Gardiner EE, Andrews RK. Platelets: envoys at the infection frontline. J Infect Dis. 2013; 208(6):871-873.
- Kelly KM, Beck SE, Pate KA, Queen SE, Dorsey JL, Adams RJ, Avery LB, Hubbard W, Tarwater PM, Mankowski JL. Neuroprotective maraviroc monotherapy in simian immunodeficiency virus-infected macaques: reduced replicating and latent SIV in the brain. AIDS. 2013; 27: F21-F28. PMCID 24051706.
- Metcalf Pate KA, Lyons CE, Dorsey JL, Queen SE, Adams RJ, Morrell CN, Mankowski JL. Thrombopoietin downregulation and elevated plasma TGFβ are associated with platelet decline in asymptomatic SIV infection JAIDS. In press. PMCID24220290
- Kelly KM, Tocchetti CG, Lyashkov A, Tarwater PM, Bedja D, Graham DR, Beck SE, Metcalf Pate KA, Queen SE, Adams RJ, Paolocci N, Mankowski JL. CCR5 Inhibition Prevents Cardiac Dysfunction in the SIV/Macaque Model of HIV. J Am Heart Assoc. 2014; 3(2):e000874. PMCID24695652.
- Dorsey JL, Mangus LM, Oakley JD, Beck, SE, Kelly KM, Queen SE, Metcalf Pate KA, Adams FJ, Marfurt CF, Mankowski JL. Loss of corneal sensory nerve fibers in SIV-infected macaques: an alternate approach to investigate HIV-induced PNS damage. AJP. In press. PMC Journal – In process.
- Metcalf Pate KA, Rice KA, Wrighten R, Watson J. Evaluation of the Effect of Sampling Strategy on the Detection of Fur Mites within a Naturally Infested Colony of Mice (Mus musculus). JAALAS. 2011; 50:337-343. PMCID21640028
- Rice KA, Chen ES, Metcalf Pate KA, Hutchinson EK, Adams RJ. Diagnosis of amyloidosis and differentiation from chronic, idiopathic enterocolitis in rhesus (Macaca mulatta) and pigtailed macaques (Macaca nemestrina). Comp Med. 2013; 63(3):262-271. PMCID23759529
- Rice KA, Brzezenski LK, Metcalf Pate KA, Perkins C, Henderson KS, Watson J. Evaluation of Diagnostic Methods for Myocoptes musculinus According to Age and Treatment Status of Mice (Mus musculus). JAALAS. 2013; 52(6):1-9. PMCID24351766.
- Baxter VK, Shaw GC, Sotuyo NP, Carlson CS, Olson EJ, Zink MC, Mankowski JL, Adams RJ, Hutchinson EK, Metcalf Pate KA. Serum Albumin and Body Weight as Biomarkers for the Antemortem Identification of Bone and Gastrointestinal Disease in the Common Marmoset PLoS One. 2013; 8(12): e82747. PMCID 24324827.