733 N. Broadway, MRB 853
Baltimore, MD 21205
410-955-9770 – Phone
443-287-2954 – Fax
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B.A., Boston University, University Professors Program, Trustee Scholars Program
D.V.M., Purdue University School of Veterinary Medicine
Ph.D., Johns Hopkins University School of Medicine
Diplomate, American College of Laboratory Animal Medicine
Dr. Metcalf Pate’s research focuses on the role of platelets in the innate immune response to viral infection, and how modulating the response of platelets to infection alters the course of disease. Platelets are tiny anuclear cells that outnumber leukocytes in the peripheral blood more than one hundred to one. Platelets are known to participate in innate immunity through cytokine signaling and direct interactions with other cells, and the platelet has the potential to significantly influence disease outcomes. However, platelet immunology is still a relatively new discipline, and the downstream effects of platelet interactions with other immune cells have yet to be determined in the context of viral infection.
The well-characterized and consistent SIV-infected pigtailed macaque model of HIV infection provides an ideal animal model in which to explore the consequences of platelet-leukocyte aggregates during acute infection. Dr. Metcalf Pate has demonstrated that 80% of CD16+ monocytes are bound to platelets during acute SIV infection, while only 6% of this population are bound in mock-inoculated controls. CD16+ monocytes are known to play important roles in the pathogenesis of SIV infection as they are infected more frequently than CD16- monocytes and transmigrate through endothelium early in infection to establish inflammatory foci in organs such as the brain and lungs. Current research aims include further characterization of the platelet-monocyte interaction during acute viral infection with the goal of establishing methods of pharmacologically manipulating this association, and establishing how platelet binding to a monocyte influences the monocyte’s susceptibility to lentiviral infection and the monocyte’s interactions with endothelium.
Dr. Metcalf Pate is additionally interested in the effect of physiologic stress on platelet function, specifically on the platelet’s future immune response to infection, and in the development and optimization of novel in vitro systems that better model in vivo conditions. She welcomes collaborative inquiries from researchers with complementary interests.
Awards and Recognition
Speaker, 2013 Gordon Seminar on Cell Biology of Megakaryocytes and Platelets
Young Investigator Travel Award, 2012 Conference on Retroviruses and Opportunistic Infections
Poster Award, 2012 Molecular and Comparative Pathobiology Year in Review, Johns Hopkins University
Claire Lyons, Undergraduate Research Fellow, Johns Hopkins University – Congratulations on Claire’s recent acceptance to Tufts University Cummings School of Veterinary Medicine!
Dr. Metcalf Pate enjoys sharing the excitement of scientific discovery with others! Local undergraduate and veterinary students with an interest in Dr. Metcalf Pate’s research are encouraged to inquire about available projects and research opportunities.
Teaching and Service
Dr. Metcalf Pate acts as an advisor within the Laboratory Animal Medicine training program (http://www.hopkinsmedicine.org/mcp/Comparative_Medicine/), and provides instruction in the following Johns Hopkins University School of Medicine courses:
- LAM/PATH Integrated Problem Solving (ME680.702), Lecturer
- The Human Body (ME800.702) (http://cmm.jhu.edu/index.php?title=Course_Work), Lecturer
- Introduction to Research Ethics (School of Medicine Graduate Student Affairs), Lecturer
- Mouse Pathobiology and Phenotyping Course (ME680:712) (http://www.hopkinsmedicine.org/mcp/PHENOCORE/phenocourseME680712.html), Laboratory Instructor
- Clinical Conference in Laboratory Animal Medicine (ME680:710), Mentor
Metcalf Pate KA, Lyons CE, Dorsey JL, Shirk EN, Queen SE, Adams RJ, Gama L, Morrell CN, Mankowski JL. In press. Platelet activation and platelet-monocyte aggregate formation contribute to platelet decline during acute SIV infection in pigtailed macaques. J Infect Dis.
Rice KA, Chen ES, Metcalf Pate KA, Hutchinson EK, Adams RJ. In press. Diagnosis of amyloidosis and differentiation from chronic, idiopathic enterocolitis in rhesus (Macaca mulatta) and pigtailed macaques (Macaca nemestrina). Comp Med.
Metcalf Pate KA, Mankowski JL. 2011. HIV and SIV associated thrombocytopenia: an expanding role for platelets in the pathogenesis of HIV. Drug Discov Today Dis Mech 8:e25-e32.
Metcalf Pate KA, Rice KA, Wrighten R, Watson J. 2011. In Search of the Elusive Myocoptes musculinus Fur Mite: Evaluation of the Effect of Sampling Strategy on the Detection of Fur Mites within a Naturally Infested Colony of Mice (Mus musculus). JAALAS 50:337-343.
Cooper CS, Metcalf Pate KA, Barat CE, Cook JA, Scorpio DG. 2009. Comparison of Side Effects between Buprenorphine and Meloxicam Used Postoperatively in Dutch Belted Rabbits (Oryctolagus cuniculus). JAALAS 48:279-285.
Warkentin KM, Buckley CM, Metcalf KA. 2006. Development of red-eyed treefrog eggs affects efficiency and choices of egg-foraging wasps. Animal Behaviour, 71:417-425.