July 25-29, 2016
Short Course & Workshop on Mouse Biology, Pathology, Genetics for Phenotyping and Translational Research
[concurrent with graduate school course ME 680.712]
At Johns Hopkins University School of Medicine,
Baltimore, MD USA
TARGET AUDIENCE: Scientists, faculty, postdocs, graduate students, veterinarians, pathologists who use or expect to use mice in translational research.
AIMS: To provide information, practical tools and skills essential to the optimal use of mice in translational research.
FORMAT & TOPICS: 5 day short-course on mouse biology, pathology, genetics, practical and multidisciplinary phenotyping for translational research, including lectures, 3 hands on laboratory session, exhibits, tours, poster session, pathology slide conference.
DVM/VMD RACE approved provider
SYLLABUS: MOUSE PATHOBIOLOGY & PHENOTYPING MANUAL is the core syllabus material, several sections can be downloaded (free)
Additional syllabus/lecture material is uploaded before the course, and provided at the course.
9am Monday to
Contact Welza1@jhmi.edu for 2016 Exhibit Information
(Grad Students, Postdocs, Faculty and Staff)
After June 1, call or email for availability of laboratory sessions.
For Graduate students and Postdocs, this is JHUSOM graduate school course ME:680.712
Faculty/Staff Tuition Remission information/registration
Early registration (before June 15):
$800 for Lectures + Hands on Laboratory Sessions, $600 for Lectures Only.
After Jun 15: $1,000 for Lectures and Lab Sessions (call or email for availability),
$800 for Lectures Only.
BWI is the closest airport
BAL is the closest Amtrak station
Local Hotel Information (this course is at Johns Hopkins Hospital or East Baltimore Campus;
733 North Broadway; Baltimore, MD 21205))
Visitor Parking (McElderry or Caroline are Closest Garages)
BRB BEHAVIOR CORE
CMOR CENTER FOR METABOLISM AND OBESITY RESEARCH
SAIRP Small Animal Imaging Resource Program
Comprehensive Oncology Tissue Services Core
Transgenic & ES Cell Core
INTRODUCTION to Mouse Pathobiology & Phenotyping
+ EXHIBITS + POSTERS
|8am-5pm||Registration - Turner Concourse |
Brayton - Introduction to phenotyping, mouse genetics, nomenclature & Famous Mice (PDF)
Web Resources: Summary Tables (doc), Famous Mice doc (PDF), Mouse Diseases doc (PDF)
Brayton - Common Spontaneous Phenotypes and Diseases (NON infectious) ( PDF)
Watson - Environmental influences on phenotypes 1
What is in your mouse environment? (PDF)
LUNCH - VISIT EXHIBITS + POSTERS
KEYNOTE LECTURE: Judith A. Hewitt, Ph.D - Chief, Research Resources Section, Office of Biodefense, Research Resources, and Translational Research/DMID/NIAID/NIH
"Enhancing Reproducibility through Rigor & Transparency"
|Watson - Environmental influences on phenotypes 2 |
What ELSE can impact your research and phenotypes: Your Surveillance Program .(PDF)
|Brayton - Infections & infectious phenotypes that can impact your research ( PDF)|
|Brayton - Immunodeficient Mice - options & challenges (PDF)|
RECEPTION, EXHIBITS &
TUESDAY July 26
GEM & Multidisciplinary Phenotyping
+ EXHIBITS, Core Tours
Reeves – Making Mice - traditional and new transgenic technologies (PDF)
Crawford / Pletnikov – Emotional / Cognitive Phenotyping (PDF)
Mitzner – Pulmonary/Respiratory Phenotypes & Phenotyping (PDF)
LUNCH & Tours - CMOR (metabolic) & BRB Imaging, Behavioral + EXHIBITS + POSTERS
Doyle - Ophthalmic Phenotyping (PDF)
Aja - Metabolic Phenotypes & Phenotyping (PDF)
Schrode / Lauer - Auditory/acoustic Phenotyping (PDF)
Foss - IMAGING for Phenotyping (PDF)
Gabrielson - Cardiovascular phenotyping: In vivo functional correlations to pathology (PDF)
WEDNESDAY July 27
Practical Phenotyping 1 DOWNLOAD LAB MANUAL - 50p PDF
Brayton - Clinical Pathology - oversimplified (PDF)
Brayton & al - Online & other Resources ( PDF)
LUNCH (on your own) & Tours - CMOR (metabolic) & BRB Imaging, Behavioral
Watson / Forbes & al
LAB 1: Clinical Examination (Lab Manual - 50p PDF)
Forbes / Brayton & al
LAB 2: Clinical Pathology and Specimen collection (Lab Manual - 50p PDF)
THURSDAY July 28
Practical Phenotyping 2 DOWNLOAD LAB MANUAL - 50p PDF
LUNCH (on your own)
Forbes / Brayton et al.
LAB 3: Anatomic Pathology - Necropsy (Lab Manual - 50p PDF)
Mangus - Basic Immunohistochemistry - principles and protocol development (PDF)
Practical Phenotyping 3
Advanced Pathology for Phenotyping & Translational Research
|Digital Slide Case Conference|
LUNCH (+ Slide Conference - Continued)
J Ward - Evaluation of Embryonic & Perinatal Lethality In Mice (PDF)
D Haines - NCI Pathology Core Support for Basic and Translational Research: GEM, Xeno, TMAs and Nano (PDF)
|3-4||T Cornish - An introduction to digital whole slide imaging and whole slide image analysis (PDF)|
MOUSE PATHOBIOLOGY & PHENOTYPING MANUAL
1st ed. 2009 and later selected sections available to download here.Section 1
Register for this course, or contact Dr. Brayton for updated revisions.
Spontaneous Phenotypes and Conditions in Common Mouse Strains & Stocks - 2009 1st ed (75pp)
Spontaneous Phenotypes and Conditions in Common Mouse Strains & Stocks - 2013 3rd ed (84pp)Section 2
Infectious Agents and Phenotypes in Mice - 2009 1st ed
Infectious Agents and Phenotypes in Mice - 2012 2nd edSection 3
Mouse Pathology Glossary: Non Neoplastic; Neoplastic - 2009 1st ed
Section 4Anatomic Pathology - Necropsy & Trimming Protocol - 2013 3rd ed (in 2013 Lab Manual)
Anatomic Pathology - Necropsy & Trimming Protocol - 2009 1st ed
Section 5Section 6
(Relatively) Non Invasive Phenotyping
Clinical Pathology Resources
Section 7Section 8
Metabolic Phenotyping Resources
Pregnancy, embryo, pup evaluation
Additional Lecture/Syllabus Material
JHU Small Animal Imaging Workshop, Mar 11-13, 2014
Brayton - Pathology Imaging PDF
2014 BTCure Workshop Vienna
Brayton - Immune Variation Among Inbred Strains and Sources PDF of PPT
2014 Mouse Models; Wellcome Trust
Immunodeficient MIce: Pathology, Phenotypes PDF of PPT
Brayton, C. (2014). Spontaneous diseases in commonly used mouse strains and stocks. MPD:Brayton1. Mouse Phenome Database web site http://phenome.jax.org. Bar Harbor, Maine USA, The Jackson Laboratory.
Brayton, C., C. Mckerlie, and S. Brown, (2014). CH 16. Analysis of Phenotype. Transgenic Animal Technology, A Laboratory Handbook. 3rd Ed. C. A. Pinkert: 431-488. Elsevier.
2013 ECVP Summer School
Mouse Pathology (Spontaneous + Infectious) text + refs 50p PDF
Mouse Pathology summary tables - 14p docx
Rat Pathology summary tables - 11p docx
2013 FELASA Practical Pathology Workshop
Practical Pathology Protocol PDF 15 pages Practical Pathology PPT PDF 15 pages
Sample Submission Forms (http://www.hopkinsmedicine.org/mcp/PHENOCORE/TESTpath.html )
Brayton, C.F., P.M. Treuting, and J.M. Ward, Pathobiology of Aging Mice and GEM: Background Strains and Experimental Design. Vet Path. 2012. 49(1): p. 85-105.
Treuting, P.M., C.B. Clifford, R.S. Sellers, and C.F. Brayton, Of mice and microflora: considerations for genetically engineered mice. Vet Pathol, 2012. 49(1): p. 44-63.
Sellers, R. S., C.B. Clifford, P.M. Treuting, and C.F. Brayton. (2012). "Immunological variation between inbred laboratory mouse strains: points to consider in phenotyping genetically immunomodified mice." Veterinary pathology 49(1): 32-43.
Brayton 2012 Mouse Cancer Models Lecture
Brayton 2012 Mouse Ageing Models Lectures
Brayton, C. and P. Treuting (2012). Phenotyping. Comparative Anatomy and Histology: A Mouse and Human Atlas P. Treuting and S. Dintzis. London, Elsevier (Academic Press): 361-381.
Danneman, P., Suckow, M.A., and Brayton, C.F. (2012). THE LABORATORY MOUSE. 2nd Ed. Boca Raton, FL, Taylor and Francis, CRC Press.
Brayton, C. (2011). Nature and Nurture: impacts on mouse phenotypes and translational research. THE MOUSE AS A MODEL ORGANISM. T. Pihlajaniemi and C. Brakebusch, Springer.