Phenotyping for Functional Genetics, ME:680.712
Course Director:
Cory Brayton, DVM, Diplomate ACLAM, ACVP
Director, Phenotyping Core
Associate Professor, Molecular and Comparative Pathobiology
JANUARY 23, 24 LABORATORY SESSIONS
Clinical and Anatomic (Pathology) Examinations for Mouse Phenotyping
Course Enrollment and Attendance of Lecture 4 are REQUIRED to participate in these sessions.
Familiarity with Necropsy Protocol in syllabus material is expected.
Wednesday January 23 | ||
| Physical Clinical Exam | SHIRPA type physical exam | Julie Watson |
| Clinical Pathology Specimen Procurement & handling | GO TO Clinical Pathology Resources | Nadine Forbes |
| Necropsy (Gross examination, tissue collection, fixation) | PDF of our necropsy protocol | Nadine Forbes |
Thursday January 24 | ||
| Processing for histology | GO TO Trimming Resources | Patricia Wilcox |
| Trimming for optimal histopathology | GO TO Trimming Resources | Nadine Forbes |
SUBMIT mouse/GEM information to JHMI GEM database
GO TO Pathology and other testing available through the
Phenotyping Core.
Clinical Pathology for Mouse Phenotyping
PDF of Dr. Brayton's handout on Hematology and differential evaluation
PDF of Ms. Forbes' handout on Hemavet and CBC recommendations
PDF of Dr. Ruben's handout of Clinical Chemistry recommendations
Additional Hematology References
Car, B. D. and V. M. Eng (2001). "Special considerations in the evaluation of the hematology and hemostasis of mutant mice." Vet Pathol 38(1): 20-30.
Doeing & al. 2003. Gender dimorphism in differential peripheral blood leukocyte counts in mice using cardiac, tail, foot, and saphenous vein puncture methods. BMC Clin Pathol. 2003; 3: 3. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201031
Everds, N in Fox. J.G. & al. Ed’s. In THE MOUSE IN BIOMEDICAL RESEARCH 2nd Ed. ACLAM series. Elsevier.
Peters, L. L., E. M. Cheever, et al. (2002). "Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice." Physiol Genomics 11(3): 185-93. (Bayer Advia)
Additional Tissue Trimming and Special Dissection resources
Davis, B. J., G. Travlos, et al. (2001). "Reproductive endocrinology and toxicological pathology over the life span of the female rodent." Toxicol Pathol 29(1): 77-83.
Foley, G. L. (2001). "Overview of male reproductive pathology." Toxicol Pathol 29(1): 49-63.
Suwa, T., A. Nyska, et al. (2002). "Spontaneous lesions in control B6C3F1 mice and recommended sectioning of male accessory sex organs." Toxicol Pathol 30(2): 228-34.
GO TO MMHCC protocols http://emice.nci.nih.gov/emice/resources/protocols
GO TO RENI Toxicologic Pathology Tissue trimming Guide
Additional Internet Resources
GO TO EMPRESS Phenotyping Platform
Clinical chemistry and haematology; Necropsy exam; etc.
GO TO NIH Virtual Mouse Necropsy
GO TO PHENOME DATABASE
Protocols + data searchable by strain, sex, age and other parameters
Additional References
Car, B. D. and V. M. Eng (2001). "Special considerations in the evaluation of the hematology and hemostasis of mutant mice." Vet Pathol 38(1): 20-30 (PUBMED).
Fox. J.G. & al. Ed’s. In THE MOUSE IN BIOMEDICAL RESEARCH 2nd Ed. ACLAM series. Elsevier. Chapters on Clinical Pathology and Diseases.
Hedrich H. Ed. 2004. THE HANDBOOK OF EXPERIMENTAL ANIMALS SERIES: THE LABORATORY MOUSE. Elsevier. Chapters on Clinical Pathology and Histology
Hrabé de Angelis, M.& al. Ed's. 2006. STANDARDS OF MOUSE MODEL PHENOTYPING. Wiley-VCH, Weinheim, ISBN 3-527-31031-2
Suckow M, Danneman P, Brayton C. 2001. THE LABORATORY MOUSE. CRC Press, Boca Raton, FL, . (Taylor & Francis, UK).
Sundberg JP & Boggess D, Ed’s. 2002. Systematic Approach to Evaluation of Mouse Mutations. CRC Press, Boca Raton, FL. (Taylor & Francis, UK) Chapter on necropsy, fixatives, trimming
Back to Phenotyping Core Home page
