Phenotyping Course Schedule 07

Lecture 4, January 17, 2008
attendance is required to participate in Lab sessions next week

Cory Brayton, DVM, Diplomate ACLAM, ACVP
Director, Phenotyping Core
Associate Professor, Molecular and Comparative Pathobiology

Clinical and Anatomic (Pathology) Examinations for Mouse Phenotyping

• How to examine a mouse
• How to prepare and submit optimal clinical pathology specimens for different tests.
• How to perform a necropsy to assess phenotypes and to submit optimal specimens for histology and other tests.

Clinical Pathology for Mouse Phenotyping
PDF of Dr. Brayton's handout on hematology and differential evaluation
PDF of Ms. Forbes' handout on Hemavet and CBC recommendations  
PDF of Dr. Ruben's handout of Clinical Chemistry recommendations

SUBMIT mouse/GEM information to JHMI GEM database

GO TO Pathology and other testing available through our Phenotyping Core.
  

Internet Resources

GO TO EMPRESS Phenotyping Platform
Clinical chemistry and haematology; Necropsy exam; etc.

GO TO NIH Virtual Mouse Necropsy

GO TO PHENOME DATABASE
Protocols + data searchable by strain, sex, age and other parameters

GO TO RENI Toxicologic Pathology Tissue trimming Guide

Additional References

Brayton, C., M. Justice, Montgomery, C. (2001). "Evaluating mutant mice: anatomic pathology." Vet Pathol 38(1): 1-19.

Car, B. D. and V. M. Eng (2001). "Special considerations in the evaluation of the hematology and hemostasis of mutant mice." Vet Pathol 38(1): 20-30 (PUBMED).

Fox.  J.G. & al. Ed’s.  In THE MOUSE IN BIOMEDICAL RESEARCH  2nd Ed.  ACLAM series.  Elsevier.  Chapters on Clinical Pathology and Diseases.

Hedrich H. Ed.  2004. THE HANDBOOK OF EXPERIMENTAL ANIMALS SERIES:  THE LABORATORY MOUSE. Elsevier.  Chapters on Clinical Pathology and Histology

Hrabé de Angelis, M.& al. Ed's. 2006. STANDARDS OF MOUSE MODEL PHENOTYPING.  Wiley-VCH, Weinheim, ISBN 3-527-31031-2

Suckow M, Danneman P, Brayton C.  2001. THE LABORATORY MOUSE. CRC Press, Boca Raton, FL, . (Taylor & Francis, UK).

Sundberg JP & Boggess D, Ed’s.  2002. Systematic Approach to Evaluation of Mouse Mutations.  CRC Press, Boca Raton, FL. (Taylor & Francis, UK) Chapter on necropsy, fixatives, trimming