LECTURE INFORMATION & SYLLABUS MATERIAL
Mouse biology genetics pathology and introductory phenotyping
3rd quarter/2nd semester: January 9- March 6, 2008
Wednesday & Thursday 2:30pm - 4:00pm
Room: BRB 801; Enrollment limited to 15.
Course Director: Cory Brayton, DVM, Diplomate ACLAM, ACVP
Lecture 1 - January 9, 2008
Cory Brayton, DVM, Diplomate ACLAM, ACVP
Director, Phenotyping Core
Associate Professor, Molecular and Comparative Pathobiology
Course format; Introduction to
- phenotyping,
- mouse biology and genetic
- Mouse/gene nomenclature and resource
PDF of Introductory powerpoint presentation
GO to additional Syllabus material and Resources
Lecture 3 - January 16, 2008
Cory Brayton, DVM, Diplomate ACLAM, ACVP
Director, Phenotyping Core
Associate Professor, Molecular and Comparative Pathobiology
Common spontaneous and infectious phenotypes that can impact research
- Phenotypes to expect with different genetic background
- Genetic backgrounds that can help or hurt a project
- Phenotypes to expect with some common infections
- Microbial agents that can help or hurt a project
PDF of Spontaneous Disease powerpoint presentation
PDF of Infectious Disease powerpoint presentation
Poster handout 1 (PDF): Microbe related phenotypes
Poster handout 2 (PDF): Background strain phenotypes
Lecture 4 - January 17, 2008
Cory Brayton, DVM, Diplomate ACLAM, ACVP
Director, Phenotyping Core
Associate Professor, Molecular and Comparative Pathobiology
Attendance required to participate in laboratory sessions
Clinical and Anatomic (Pathology) Examinations for Mouse Phenotyping
- How to examine a mouse
- How to prepare and submit optimal clinical pathology specimens for different tests.
- How to perform a necropsy to assess phenotypes and to submit optimal specimens for histology and other tests
PDF of Clinical and Anatomic Pathology PPT presentation (25 pp)
PDF of Pathology (Necropsy) Protocol (11 pp)
SYLLABUS MATERIAL (relevant to the 1st 6 lecture and laboratory sessions) :
Outline (PDF) of Spontaneous Diseases in Commonly Used Background Strains and Stocks
(75 pages - Jan 7, 2008 update)?
Outline (PDF) of Infectious Agents and Diseases in Mice
(37 pages - Jan 7, 2008 update)
A Glossary of Mouse Pathology
(40 pages - Jan 7, 2008 update)
INTERNET RESOURCES YOU SHOULD USE
NEW JHMI GEM Database initiative?
Complete this form to submit GEM information to the JHMI GEM site
Mouse/Gene Nomenclature
- Information to find/use correct names for publication, grants, communication;
- Search for Lab code information, apply for your own lab code.
Search MGI for genes, markers, etc
- current names, history, homologies, alleles
Search IMSR for special stocks and strains in Repositories
- current names, history, availability, references
Phenome Database
- Phenotype Data on Prioritized inbred strains
- growth curves, organ weights, CBC, glucose, body condition, behavioral etc
FIND YOUR MICE & THEIR HEALTH REPORTS at:
FIND YOUR GEM at:
Deltagen and Lexicon Knockout Mice and Phenotypic Data
Jax IMSR (Induced Mutant Strain Resource)
MMRRC Mutant Mouse Regional Resource Centers
More Online Resources:
Edinburgh Mouse Atlas: Digital Atlas of Mouse Development
EUMORPHIA EMPRESS (European Mouse Phenotyping Resource of Standardised Screens)
EUrophenome Mouse Phenotyping Resource
EUMODIC (European Mouse Disease Clinic) Phenotyping Initiative
Jax - The Jackson Laboratory - Community Resources?
JAX ES Cell lines & strains of origin
JAX Festings information on inbred strains
JAX Lee Silver's MOUSE GENETICS on line
JAX Mouse Phenome database (prioritized strains, protocols, data, more)
JAX Willys Silver's?(1979) COAT?COLORS?OF?MICE??
NCI MMHCC (Mouse Models of Human Cancer Consortium) Site
(Model information with links to Consensus papers, protocols, etc)
NIH KOMP (Knockout Mouse Project)
NorCOMM (North American Conditional Mutant Mouse Project)
Pathbase: European mutant mouse pathology database
Additional References
Barthold, S. W. (2002). "Muromics": genomics from the perspective of the laboratory mouse." Comp Med 52(3): 206-23.
Barthold, S. W. (2004). "Genetically altered mice: phenotypes, no phenotypes, and Faux phenotypes." Genetica 122(1): 75-88.
Brayton C. ?Spontaneous diseases in commonly used inbred mouse strains.??2006. In Fox. & al. Eds. In THE MOUSE IN BIOMEDICAL RESEARCH 2nd Ed. ACLAM series. Elsevier.
Hedrich H. Ed.? 2004. THE HANDBOOK OF EXPERIMENTAL ANIMALS SERIES:? THE LABORATORY MOUSE. Elsevier.
Hrab? de Angelis, M.?&?al. Eds.?2006. STANDARDS OF MOUSE MODEL PHENOTYPING.?Wiley-VCH, Weinheim, ISBN 3-527-31031-2
Kaufman MH.? 1992.? ATLAS OF MOUSE DEVELOPMENT. Academic Press
Maronpot, R. & al., Eds. (1999). PATHOLOGY OF THE MOUSE: REFERENCE AND ATLAS. Cache River Press. Vienna, IL
Papaioannou VE, Behringer RR. 2004. MOUSE PHENOTYPES: A HANDBOOK OF MUTATION ANALYSIS. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY (review in Nature)
Smith, R. S. (2001). Systemic Evaluation of the Mouse Eye, Anatomy, Pathology, and Biomethods. Boca Raton, FL., CRC Press, (Taylor & Francis, UK).
Suckow M, Danneman P, Brayton C.? 2001. THE LABORATORY MOUSE. CRC Press, Boca Raton, FL, . (Taylor & Francis, UK).
Sundberg JP, Boggess D, Ed s. 2002. Systematic Approach to Evaluation of Mouse Mutations. CRC Press, Boca Raton, FL. (Taylor & Francis, UK)
Sundberg, J. and T. Ichiki (2005). Genetically Engineered Mice Handbook. CRC Press; Boca Raton, FL (Taylor & Francis, UK).
Ward JM, Maronpot RR, Sundberg JP.? 2000.?? PATHOLOGY OF GENETICALLY ENGINEERED MICE. Ames, IA, Iowa State University Press (Blackwell Publishing
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