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MCP (BRB8) Histology Laboratory

Location: BRB 856
Contact: Pat Wilcox , ASCP, X5-8728 or pwilcox@jhmi.edu

Consult a Pathologist (for research pathology)

Procedures, contacts, costs, specimens

Procedure

Contact

Cost

Specimen

Additional (recut) H&E slides$1.20Block
Cover Slipping (plastic tape)$1.00 for 1-10Slides
Cover Slipping (Glass)$1.00Slide
Decalcify (Formic acid -sodium citrate method)
$3.75/wk or piece
Boney Tissue
Decalcify (other method)varies
Boney Tissue
Deparaffinize (from unstained slide)pwilcox@jhmi.edu$0.70Unstained Slide
Process Fixed Tissue in cassette, Embed onlypwilcox@jhmi.edu
$3.00

Tissue in Cassette

Process Fixed Tissue in cassette, Embed, 1 H&E
$5.70
Tissue in cassette
Process Fixed Tissue in cassette, Embed,
+ Special requests
From $5.00
Tissue in cassette
Serial Sectioning
$5.70
Block
Slide Box (5 slide plastic mailer)
pwilcox@jhmi.edu$1.50 
Slide Box (100 slides)
$30.00
 
Slide Flat (20 slide cardboard flat)pwilcox@jhmi.edu$5.00 
Special Processing (re - embedding etc)
pwilcox@jhmi.edu
From $3.00
Block
Special Stains (See Below)varies
Block or Unstained Slide
Step Sectioning - cutting fee
from $4.00 for up to 5 /block
Block
Trimming Tissue into cassettes
$2.00/cassette
Wet tissues
Recut H&E slides
$1.20
Block
Unstained Sections from previously embedded blocks (for IHC etc)
pwilcox@jhmi.edu
$2.00
Block
Unstained Slides on
    Fisher Plus Slides
    "Chem mate", Selane, poly-L-lysine coated
   
pwilcox@jhmi.edu
+ $0.60
+ $0.75

Block

Special Stains (processing, sectioning are separate charges)

Special Stains (processing, sectioning are separate charges)
Each section / slide from previously embedded blocks
pwilcox@jhmi.edu$0.60Block
Special Stains (no Silver)
Most <$3.00
Unstained Slide
Special Stains (with Silver)
pwilcox@jhmi.eduFrom $3.00Unstained Slide
Acid Fast - Fite Faraco
$2.50
Unstained Slide
Acid Fast - Ziehl Neelsenpwilcox@jhmi.edu
$2.00
Unstained Slide
Alcian Blue (stains mucin mucoproteins blue dt copper)
$2.00
Unstained Slide
Alizarin Red (anthraquinone derivative stains calcium red)
$2.00
Unstained Slide
Azure-Eosin (Giemsa-like for organisms)$2.50
Unstained Slide
Congo Red with Glass Cover Slip
(--> red amyloid with green birefringence)
$3.00
Unstained Slide
Eosin Only
$0.70
Unstained Slide
Feulgen (for DNA)pwilcox@jhmi.edu
$3.00
Unstained Slide
GMS (Gomori Methenamine Silver for fungus etc)
$2.50
Unstained Slide
Gram (Brown Hopps modified)
$2.00
Unstained Slide
HARTS (for elastin etc)
$2.50
Unstained Slide
LFB - Luxol Fast Blue (for myelin etc)
$3.00
Unstained Slide
LFB PAS
$4.00Unstained Slide
Nissl (basic dyes stain RNA, DNA)
pwilcox@jhmi.edu
$2.00
Unstained Slide
PAMS (PAS + Methenamine Silver)
$4.00
Unstained Slide
PAS (Periodic Acid Schiff - magenta for glycogen,  chitin, mucin, BM etc )$2.00
Unstained Slide
Pentachrome (for connective tissue)$4.00
Unstained Slide
PTAH (Phosphotungstic Acid Hematoxylin for striated muscle, mitochondria (blue))
$2.00
Unstained Slide
Prussian Blue (Perl's reaction for ferric irron, ferritin, hemosiderin)pwilcox@jhmi.edu
$2.00
Unstained Slide
Reticulin stain (silver for reticulin fibers)$3.00
Unstained Slide
Picrosirius Red with Glass Cover slip
(collagen birefringence (green-yellow-orange) with polarized light)
$3.50
Unstained Slide

Safranin O (stains mucin, cartilage, mast cells orange/red)

$3.00
Unstained Slide
Sudan Black for lipid$3.00Unstained Slide
Sevier Munger (silver for nervous tissue, enterochromaffin, C cells, etc)$3.00
Unstained Slide
Toluidine blue (mast cells, cartilage, etc)$2.00
Unstained Slide
Trichrome (Mallory's - Collagen is blue)$2.50
Unstained Slide
Von Kossa (silver for Calcium)$3.00
Unstained Slide
VVG Verhoeff Van Gieson (connective tissue)$2.50
Unstained Slide
Warthin Starry (argyrophilic bacteria)$3.00
Unstained Slide

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