Tissue microarrays (TMAs) allow rapid screening of gene expression or chromosomal alterations from a large number of tissue samples on a single microscope slide. The TMA Core at Johns Hopkins was initially funded by the Prostate SPORE but has grown into a user-supported University Service Center/Shared Resource. Since 2005 The TMA Lab has constructed two hundred additional TMAs bringing the total to 598 for 43 (additonal 17) investigators containing 93,000(40,000 additional) tissue cores from 10,000 Pathology cases containing tumors from the prostate, breast, pancreas, gall bladder, cervix, ovary, brain, liver, urinary bladder, salivary glands, esophagus, head and neck, thyroid and various cell lines. Users have published 118 manuscripts involving JHU TMAs.
Instrumentation is available to automatically capture high resolution digital images of TMA spots and perform semi-automated quantitative image analysis. The current number of composite TMA images stored server is 288,978 from 8098 TMA slides. Increased from 104,059 from 84 different TMAs. Users have access to a set of Open Source web-based software tools and backend database (TMAJ) that were developed to facilitate the all facets of research involving TMA technology from TMA design, data entry, image diagnosis, analysis, and publishing on the Internet.
The Specific Aims of this Shared Resource are to support and speed translational cancer research by :
- continuing to produce TMAs for SKCCC members and others using human surgical and autopsy specimens, human specimens from clinical trials, xenografts, animal tissue specimens and cell lines as needed;
- provide services to allow in situ visualization of novel cancer-associated DNA and protein biomarkers
- to continue to evaluate and update new TMA construction and imaging platforms;
- to enhance the Core’s information technology platform to improve ease of use, compliance with emerging CaBIG standards, including common data elements, and integration of new image analysis technologies with TMAJ.
New immunohistochemistry services have been incorporated into the TMA Core and to the TMA Core space. The Core offers better coverage, shorter turn-around times and enhanced services, with more continuous coverage. Technicians can perform the tasks in these three areas, Histology, TMA construction and IHC.
Angelo M. De Marzo, M.D., Ph.D., Resource Director
Marc K. Halushka, M.D., Ph.D., Co-Resource Director
Alan Meeker, Director of IHC Facility
Helen Fedor, B.S., Resource Manager