Dr. Linzhao Cheng
Professor of Gynecology and Obstetrics and Oncology
Ph.D., Molecular Biology and Genetics, Johns Hopkins University, Baltimore, MD
Investigate molecular mechanisms controlling self-renewal of human
hematopoietic stem cell (HSC), by using postnatal human HSCs directly or
human embryonic stem (hES) cells capable of self-renewal proliferation in
culture indefinitely; Improve gene transfer into HSCs & hES cells and regulated gene
expression in stem cell-derived progenies for gene therapy and laboratory
research; Modulate immune responses using stem cells or their antigen-presenting
cell (APC) progeny to induce immune tolerance, in order to improve
transplantation acceptance of HSCs, hES cells and their derivatives.
Overall Research Summary and Significance:This lab employs an array of molecular techniques to study molecular mechanisms regulating stem cell self-renewal, and to functionally enhance stem cells for better therapeutic potentials. Dr. Cheng contributed the recent breakthrough to target gene expression in specific differentiation lineage derived in vivo from transduced pluripotent stem cells. Together with two recent papers (one in Nature, 5/2001 and one in Science, 11/2001) showing targeted gene expression in red blood cells after HSC transduction by similar vectors, this marks the beginning of a new era of regulated gene therapy and targeted use of pluripotent stem cells for a safer and better therapy.
Cui Y, Golob J, Kelleher E, Ye Z, Pardoll D and Cheng L (2002). Targeting transgene expression to antigen presenting cells derived from lentivirus transduced, engrafting human hematopoietic stem/progenitor cells. Blood, Vol. 99, 399-408.
Yang Y, Wang W, Cleaves R, Zahurak M, Cheng L, Civin C and Friedman A (2002). Acceleration of G1 cooperates with core binding factor b-smooth muscle myosin heavy chain to induce acute leukemia in mice. Cancer Research, Vol. 62(8): 2232-2235.
Bernardin F, Yang Y, Cleaves R, Zahurak M, Cheng L, Civin C and Friedman A (2002). TEL-AML1, expressed from t (12;21) in human acute lymphocytic leukemia, induces acute leukemia in mice. Cancer Research, 62(14):3904-3908.