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 Sample Molecular Epidemiology Laboratory Report (Dowload the PDF)

Date:

5/27/99

 

Method:

PFGE

 

% Agarose:

1%

Location:

ICU-A

 

Enzyme:

SmaI

 

Buffer:

.5X

Organism:

MRSA

 

Pulse Time:

5 to 40 sec

 

Tech Initials:

sh

Gel No.:

1

 

Run Time:

24 hours

 

Reviewer Initials:

wgm


Description of Isolates:

Lane

Name

Date

Specimen No.

Body Source

Comment

1

Molec. Wt. Marker

 

 

 

E. faecalis OGIRF

2

Patient 1

10/19/96

 

endoctracheal

 

3

Patient 2

6/20/97

 

nares

 

4

Patient 3

6/20/97

 

nares

 

5

Patient 4

5/27/97

 

blood

 

6

Patient 5

6/20/97

 

nares

 

7

Patient 6

6/17/97

 

blood

 

8

HCW 1

6/26/97

 

nares

 

9

HCW 2

6/30/97

 

nares

 

10

Molec. Wt. Marker

 

 

 

E. faecalis OGIRF


Epidemiologic Question:

Is the MRSA from Patient 1, isolated 10/96 at Hospital A, related to a cluster of MRSA cases (Patients 2 to 6) from May and June of 1997? Two health care workers (HCW) were colonized with MRSA at the time the cluster was discovered. Are the isolates from HCW1 and HCW2 related to patient strains?


              
1
2
3
4
5
6
7
8
9
10

                                        
Interpretation:

MRSA isolated in 1997 from Patients 2,3,4,5, and 6 are indistinguishable. The MRSA from HCW2 has the same fingerprint pattern as the clustered patients. These six cases are the same strain type ICU-A. Patient 1 has a closely related isolate, subtype ICU-A1. HCW1 also has a strain closely related to the outbreak, ICU-A2.

 

 

 

 

 

 

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