Sample Report

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Sample Molecular Epidemiology Laboratory Report (Dowload the PDF)

Date:	5/27/99	Method:	PFGE	% Agarose:	1%
Location:	ICU-A	Enzyme:	SmaI	Buffer:	.5X
Organism:	MRSA	Pulse Time:	5 to 40 sec	Tech Initials:	sh
Gel No.:	1	Run Time:	24 hours	Reviewer Initials:	wgm

Description of Isolates:

Lane	Name	Date	Specimen No.	Body Source	Comment
1	Molec. Wt. Marker				E. faecalis OGIRF
2	Patient 1	10/19/96		endoctracheal
3	Patient 2	6/20/97		nares
4	Patient 3	6/20/97		nares
5	Patient 4	5/27/97		blood
6	Patient 5	6/20/97		nares
7	Patient 6	6/17/97		blood
8	HCW 1	6/26/97		nares
9	HCW 2	6/30/97		nares
10	Molec. Wt. Marker				E. faecalis OGIRF

Epidemiologic Question:

Is the MRSA from Patient 1, isolated 10/96 at Hospital A, related to a cluster of MRSA cases (Patients 2 to 6) from May and June of 1997? Two health care workers (HCW) were colonized with MRSA at the time the cluster was discovered. Are the isolates from HCW1 and HCW2 related to patient strains?

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Interpretation:

MRSA isolated in 1997 from Patients 2,3,4,5, and 6 are indistinguishable. The MRSA from HCW2 has the same fingerprint pattern as the clustered patients. These six cases are the same strain type ICU-A. Patient 1 has a closely related isolate, subtype ICU-A1. HCW1 also has a strain closely related to the outbreak, ICU-A2.