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Genetics Resources Core Facility

Core Symposium 2012 
Click here for symposium guide

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The Genetic Resources Core Facility (GRCF) is a JHU service center including the Core Store and five Research Services.  Collectively, these divisions offer a wide variety of products and services to all Hopkins researchers. The divisions of the GRCF include:



Visit the facility's Web site



The Genetic Resources Core Facility (GRCF) is a JHU service center including the Core Store and five Research Services.  Collectively, these divisions offer a wide variety of products and services to all Hopkins researchers. The divisions of the GRCF include:

Core Store provides one-stop shopping for more than 100,000 products from 16 of the leading life science companies.  In addition to its product offering the store charges no shipping and handling fees and has free delivery to three campuses; East Baltimore, Bayview and Homewood. There is also, convenient 24/7 access to several hundred products via the Core Store 24/7 at these locations; Blalock 1026, CRB I-B02A and the Asthma and Allergy Building 1st floor. For more information go to www.jhucorestore.com

Cell Center and Biorepository provides the research community at Johns Hopkins University with access to cell line establishment and storage of aliquots generated from whole blood lymphocytes and skin fibroblasts. The Center has the capability to generate lymphoblastic cell lines from peripheral blood B-cells with high efficiency at an extremely competitive cost.  Current offerings also include isolation and storage of sera, plasma, and peripheral blood mononuclear cells from whole blood.  Expansion of mycoplasma-free cultures for seed or working stocks is available. Worldwide shipping is available in either dry ice or dry shipper formats for all repository customers.  Free project consultation is available. For more information go to http://cellcenter.grcf.jhmi.edu

DNA Analysis Facility provides on-site same day or next day Sanger-based sequencing, Pyrosequencing (for methylation studies or areas difficult to genotype), low cost oligonucleotide synthesis through partnerships with Bioneer, IDT, Operon and Sigma-Genosys, access to a self service Applied Biosystems 7900 machine for Real-time PCR applications, and easy ordering of siRNAs, shRNAs, miRNAs and qPCR primers from QIAGEN and Sigma. Discounted pricing on Taqman assay are also available through Applied Biosystems.  Gene synthesis services are available through both Bioneer and IDT.  For more information please go to http://daf.jhmi.edu

Fragment Analysis Facility provides genomic DNA isolation, low through-put genotyping including SNP, STR and VNTR analysis, cell line authentication, mycoplasma testing, whole genome amplification, bisulfite conversion and DNA storage. For more information go to http://faf.grcf.jhmi.edu

High Throughput Sequencing was established in 2008 to provide the research community at Johns Hopkins University with access to 'next generation' sequencing platforms. They feature three Illumina HiSeq2000s, which have the capability to generate sequences with high accuracy at an extremely low cost per base.  Current offerings include whole exome/custom capture sequencing and analysis, whole genome sequencing, and RNAseq.  Free project consultation is available.  For more information go to http://grcf.jhmi.edu/hts

SNP Center high through-put genotyping services using the Illumina BeadArrayTM technology to both the JHU and larger scientific communities.  The lab has the capacity to produce millions of genotypes on hundreds of samples each week.  No cost access to the SNP Center can be obtained through our CIDR (http://www.cidr.jhmi.edu/) contract.  For more information go to http://snpcenter.grcf.jhmi.edu


Visit the facility's Web site



Time:

3:00 p.m. - 4:00 p.m.

Location:

BRB G-01

Presented by:

Director of Research, Knome, Inc.

Nathaniel Pearson, Ph.D



The Genetic Resources Core Facility (GRCF) is a JHU service center including the Core Store and five Research Services.  Collectively, these divisions offer a wide variety of products and services to all Hopkins researchers. The divisions of the GRCF include:

Core Store provides one-stop shopping for more than 100,000 products from 16 of the leading life science companies.  In addition to its product offering the store charges no shipping and handling fees and has free delivery to three campuses; East Baltimore, Bayview and Homewood. There is also, convenient 24/7 access to several hundred products via the Core Store 24/7 at these locations; Blalock 1026, CRB I-B02A and the Asthma and Allergy Building 1st floor. For more information go to www.jhucorestore.com

Cell Center and Biorepository provides the research community at Johns Hopkins University with access to cell line establishment and storage of aliquots generated from whole blood lymphocytes and skin fibroblasts. The Center has the capability to generate lymphoblastic cell lines from peripheral blood B-cells with high efficiency at an extremely competitive cost.  Current offerings also include isolation and storage of sera, plasma, and peripheral blood mononuclear cells from whole blood.  Expansion of mycoplasma-free cultures for seed or working stocks is available. Worldwide shipping is available in either dry ice or dry shipper formats for all repository customers.  Free project consultation is available. For more information go to http://cellcenter.grcf.jhmi.edu

DNA Analysis Facility provides on-site same day or next day Sanger-based sequencing, Pyrosequencing (for methylation studies or areas difficult to genotype), low cost oligonucleotide synthesis through partnerships with Bioneer, IDT, Operon and Sigma-Genosys, access to a self service Applied Biosystems 7900 machine for Real-time PCR applications, and easy ordering of siRNAs, shRNAs, miRNAs and qPCR primers from QIAGEN and Sigma. Discounted pricing on Taqman assay are also available through Applied Biosystems.  Gene synthesis services are available through both Bioneer and IDT.  For more information please go to http://daf.jhmi.edu

Fragment Analysis Facility provides genomic DNA isolation, low through-put genotyping including SNP, STR and VNTR analysis, cell line authentication, mycoplasma testing, whole genome amplification, bisulfite conversion and DNA storage. For more information go to http://faf.grcf.jhmi.edu

High Throughput Sequencing was established in 2008 to provide the research community at Johns Hopkins University with access to 'next generation' sequencing platforms. They feature three Illumina HiSeq2000s, which have the capability to generate sequences with high accuracy at an extremely low cost per base.  Current offerings include whole exome/custom capture sequencing and analysis, whole genome sequencing, and RNAseq.  Free project consultation is available.  For more information go to http://grcf.jhmi.edu/hts

SNP Center high through-put genotyping services using the Illumina BeadArrayTM technology to both the JHU and larger scientific communities.  The lab has the capacity to produce millions of genotypes on hundreds of samples each week.  No cost access to the SNP Center can be obtained through our CIDR (http://www.cidr.jhmi.edu/) contract.  For more information go to http://snpcenter.grcf.jhmi.edu


Visit the facility's Web site

 
 
 
 
 

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