Gail Stetten, Ph.D.

Education:

A.B., Rutgers University, 1967
Ph.D., Brown University, 1972
Postdoc, Harvard Medical School, 1976

Main Interests:

My research interests focus on mechanisms leading to human chromosome abnormalities, specifically de novo rearrangements.Traditional and molecular methods as well as chromosomal microarrays are being used to help evaluate unusual chromosomes and correlate the clinical phenotype to abnormalities in the karyotype. Human embryonic stem cell lines are also being routinely karyotyped in our lab.Fluorescent in situ hybridization (FISH) methods are being used in collaborative mapping studies.

In work concerning mouse models of human disease, we are evaluating chromosomes from embryonic stem cell lines for both karyotypic status and construct integration site prior to transgenesis. An interphase FISH method has been developed to rapidly determine specific aneuploidy in mice. Presently this is being used to determine chromosome 16 copy number in a mouse model for Down syndrome. 
  
Research Interests:

• Complex chromosome rearrangements
• Chromosome fragility
• Ring chromosome formation
• Accessory marker chromosomes
• Centromeres and telomeres  

Clinical Activities:

• Board Certification: American Board of Medical Genetics, Clinical Cytogenetics, 1982
• Clinical Interests: Director, Prenatal Cytogenetics Laboratory 

Educational Activities:

• Preceptor, Medical Genetics Training Program
• Cytogenetics lecture in Molecules and Cells course
• Resident teaching for Gynecology and Obstetrics, Pathology 

Recognition and Leadership Roles:

• 1990 Chromosoma Prize for the best paper published in 1989
• Reviewer, American Journal of Medical Genetics 
• Editor, Genetic Testing

Publications:

• Earnshaw WC, Ratrie H, Stetten G: Visualization of centromere proteins CENP-B and CENP-C on a stable dicentric chromosome in cytological spreads. Chromosoma 98:1-12, 1989. Abstract
• Rosenberg C, Blakemore KJ, Kearns WG, Giraldez RA, Escallon CS, Pearson PL, Stetten G : Analysis of reciprocal translocations using DNA libraries: Applications and limitations of the technique. Am J Hum Genet 50:700-705 , 1992. Abstract
• Blakemore KJ, Rosenberg C, Jaswaney VL, Pressman EK, Kearns WG, Pearson PL, Stetten G: Rapid diagnosis of trisomy 18 and dizygosity in twins using fluorescence in situ hybridization on uncultured amniocytes. J Maternal-Fetal Medicine 2:197-200, 1993.
• Batista DAS, Tuck-Muller CM, Martinez JE, Kearns WG, Pearson PL, Stetten G: A complex chromosomal rearrangement detected prenatally and studied by fluorescence in situ hybridization. Hum Genet 92:117-121 , 1993. Abstract
• Batista DAS, Pai GS, Stetten G: Molecular analysis of a complex chromosomal rearrangement and a review of familial cases. Am J of Med Genet 53:255-263, 1994. Abstract
• Batista DAS, Escallon C, Blakemore KJ, Stetten G: An accessory marker derived from chromosome 20 and its coexistence with a mosaic trisomy 20 cell line. Prenat Diagn 15:123-127, 1995. Abstract
• Stetten G, Charity LC, Kasch L, Scott AF, Berman C, Pressman E, Blakemore KJ: A paternally derived inverted duplication of 7q with evidence of a telomeric deletion. Am J Med Genet 68:76-81, 1997. Abstract 
• Stetten G, Goodman BK, Fox HE: New cytogenetic technology and its application in maternal-fetal medicine. J Maternal-Fetal Invest 7(4):155- 162 , 1997.
• Goodman BK, Stone KM, Coddett JM, Cargile C, Gurewitsch ED, Blakemore KJ, Stetten G: Molecular cytogenetic analysis and clinical findings in a newborn with prenatally diagnosed rec(7)dup(7q)inv(7)(p22q31.3)pat. Prenat Diagn 19:1150-1156, 1999. Abstract
• Tuck-Muller CM,Goodman BK, Shibo L, Martinez JE, Chen X-N, Wertelecki W, Korenberg JR, Stetten G: Partial trisomy 7p defined by analysis of a complex chromosome rearrangement (CCR) using a BAC clone panel. Genet Med 3:126-131, 2001. Abstract
• Stetten G, Escallon CS, South, ST, McMichael JL, Saul DO and Blakemore KJ  (2004)  Reevaluating confined placental mosaicism. Am J Med Genet 131A:232-239.
• South ST, Corson VL, McMichael JL, Blakemore KJ and Stetten G  (2005)  Prenatal detection of an interstitial deletion in 4p15 in a fetus with an increased nuchal skin fold measurement.  Fetal Diagnosis and Therapy 20:58-63.
• Cain CC, Saul D, Attanasio L, Oehler E, Hamosh A, Blakemore K and Stetten G (2007)  Microphthalmia with linear skin defects (MLS) syndrome evaluated by prenatal karyotyping, FISH and array comparative genomic hybridization. Prenat Diagn27:373-379.

Contact Information:

Gail Stetten, PhD

Park Building B-2
600 N. Wolfe Street
Baltimore, MD 21287-2501
Phone: (410) 955-3386
Fax: (410) 614-8766
E-Mail: gstetten@jhmi.edu

Page last updated:07/23/2008