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School of Medicine
FACS Aria IIu Sorter
The FACS Aria sorter equips with three lasers (488 nm, 633 nm and 405 nm), and optical filters up to 9 fluorescent parameters (Configuration). FACS Diva software is used for cell collection and analysis. It was upgraded to the level of II in July 2012. It is operated by a designated operator, and able to sort 4 different cell populations simultaneously. It is operated under the BSL2, and applicable to sorting both human and rodent cells (see Biosafety Policies).
The LSR II is equipped with the following four lasers: sapphire (blue, excitation at 488 nm), HeNe (red, excitation at 633 nm), violet (excitation at 405 nm) and ultraviolet (excitation at 355 nm) (Configuration and Fluorochrome Chart). FACS Diva software (PC) is used for data acquisition and analysis. The instrument is accessible for unassisted use 24 hours a day, 7 days a week.
The FACSCalibur is equipped with two lasers, blue and red, allowing for excitation at 488 nm and 633 nm, respectively. Four channels can be set for fluorescent parameters (Configuration). CellQuest software (Mac) is used for sample acquisition and data analysis. The instrument is accessible for unassisted use 24 hours a day, 7 days a week.
The core also provides data analysis software of FlowJo, FACSDiva, and CellQuest workstations to the core users. Click here for the FACSDiva Software Quick Reference Guide.