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Integrated Physiology

Andrew Wolfe, Ph.D., Core Director
Timothy Moran, Ph.D., Core Co-Director

The Integrated Physiology Core of the JHU-UMD Diabetes Research Center provides investigators with essential support by facilitating and enhancing studies that seek to understand the physiological consequences of cellular and molecular changes incurred with diabetes and its attendant disorders.

The objectives of the Integrated Physiology Core are:
1. To enhance the cost effectiveness of a variety of metabolic and hormone assay for center investigators.

2. To make available assay procedures that would be impractical or not feasible for center investigators to establish and perform in their own laboratory.

3. To develop new techniques or protocols to enhance the projects of center investigators.

4. To function seamlessly with the Transgenics/ES Core in the analysis of newly developed animal models.

5. To closely coordinate with the Cell Biology Core for ligand assay purposes. An added advantage will be the ability of Center Investigators to design, analyze and execute studies that look at both in vitro (Cell Biology Core) and in vivo (Integrated Physiology Core) aspects of metabolic function.

Services offered:

1. Assays of rodent physiology and behavior, including assessment of VO2, VCO2 and RER (respiratory exchange ratio), activity monitoring and food consumption.

2. Ligand assay measurements of hormones and metabolic substrates such as glucose and FFA, including new assay development

3. Assessments of glucose metabolism including glucose and insulin measurements, and glucose and insulin tolerance tests. The Core will also train investigators to perform these tests in their laboratory.

4. Performing or teaching investigators to perform various endocrine surgical services including, but not limited to, jugular catheterization, gonadectomy, rodent dissection and tissue isolation.

5. Animal imaging of body composition and substrate use.

6. Consultation with investigators regarding experimental protocols for their metabolic and endocrine related studies and assistance with the design, formulation and application of specialized diet paradigms as well as the implementation of food and water restriction schedules.