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Diabetes Research Center

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Integrated Physiology

Ligand Assay and Biomarker Sub-Core

Director

Andrew Wolfe, Ph.D.
awolfe3@jhmi.edu

Luminex Multiple Ligand Assays
ELISA Assays
Lipid and cholesterol assays

Multiplex Assay Detection Systems

(Luminex platform, BioTek platform)

Comprehensive Laboratory Animal Monitoring System (CLAMS)

Food intake
Paired feeding
Locomotion, circadian activity
Resting energy expenditure
Oxygen consumption
Carbon dioxide production
Heat generation
Training in CLAMS and investigator-directed CLAMS
Full-service CLAMS protocols are available as an independent service.

Body Composition

(DEXA, qMRI)


Rodent Physiology and Behavioral Analysis Subcore

Director

Susan Aja, Ph.D.

Food intake
Paired feeding
Locomotion, circadian activity
Resting energy expenditure
Oxygen consumption
Carbon dioside production
Heat generation
Training in CLAMS and infestigator-firected CLAMS
Full-service CLAMS protocols are available as an independent service.


Glucose Metabolism Analysis Subcore

Director

Sheng Bi, M.D., M.M.
sbi@jhmi.edu

Expertise, training and advice in:

Intra-peritonal glucose tolerance test
Oral gavage
Oral glucose tolerance test
Insulin secretion test
Tracer-Based In Vivo Substrate Handling
Glucose Clamp in Rodents

Dynamic Physiologic Testing

Expertise, training and advice in:

Intraperitonal glucose tolerance test
Oral gavage
Oral glucose tolerance test
Insulin tolerance test
Pyruvate conversion test
Insulin secretion test
Tracer-Based In Vivo Substrate Handling
Euglycemic Clamp in Rodents
Optogentic Selective Neuronal Stimulation