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Diabetes Research Center

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Cell Biology

Director

Mehboob Hussain, M.D.
mhussai4@jhmi.edu

Co-Director

Ling He, M.D., Ph.D.
heling@jhmi.edu

Tissue Analysis and Imaging

Tissue dissection
Fixation
Cryosectioning
Immunostaining
Morphometric analysis
Immunofluorescence imaging
Confocal microsocpy

Islet Biology

Islet isolation (rodent) and culture expertise and training
Insulin secretion assays: static incubation, perifusion assays
Maintenance of cell lines and culture/transfection protocols
Maintenance of relevant transgenic mouse lines, i.e., cell-specific CRE driver lines

Liver Biology

Hepatocylte isolation and culture expertise and training
Liver-directed gene delivery protocols and expertise
Maintenance of cell lines and culture/transfection protocols

Adipocyte Biology

Housed in Mid-Atlantic Nutrition and Obesity Research Center at the University of Maryland

Derivation of IPSC

Independent core at Johns Hopkins University Institute for Cell Engineering

Bioenergetics

Independent core in Department of Physiology at The Johns Hopkins University


Bone Biology Subcore

Director

Ryan Riddle, Ph.D.
rriddle@jhmi.edu

Micro CT for bone mineral density in animal models
Bone strength/fracture measurements
Bone morphometric analyses
Osteoblast isolation and culture expertise
Maintenance of relevant transgenic mouse lines, i.e., cell-specific CRE driver lines


Specialty Animal Surgery Subcore

Director

Gerald Brandacher, M.D.
brandacher@jhmi.edu

Parabiosis surgery
Partial pancreatectomy
Kidney transplantation
Selective vagotomy
Bariatric surgery in rodents
Transplantation of tissue under the kidney capsule
Jugular and/or Inguinal venous and arterial cannulation in awake and freely moving rodents