|While we study how these processes direct cytokinesis, we are also learning how these same principles apply to diseases such as cancer, lung and motor neuron diseases. To accomplish such broad goals, we collaborate closely with a several basic and clinical scientists. For example, with Mike Overholtzer (Memorial Sloan Kettering), we determined how mechanical cues guide aberrant behaviors in breast cancer. Here, we found that cancer and non-cancer cells compete with each other, and due to their unique mechanical properties, the cancer cell can engulf and kill the non-cancer cell . Working with Bob Anders (JHU Pathology), we are examining how changes in cell mechanics correlate with pancreatic ductal adenocarcinoma cancer (PDAC) progression. In this context, key molecular changes associated with disease progression are also known to regulate central elements of the cell's contractile machinery. We are finding that many of the mechanosensory proteins undergo dramatic changes in expression as a normal pancreatic ductal epithelial cell progresses to metastatic disease. With Ramana Sidhaye (JHU Pulmonology), we are exploring the acute changes to cellular architecture that occur in the lung epithelia in response to insults such as cigarette smoke, which can ultimately lead to diseases such as chronic obstructive pulmonary disease (COPD) and lung cancer . Fascinatingly, many of the same principles apply to degenerative motor neuron disease, and we have found a way to apply our fundamental discoveries here too by working with Charlotte Sumner and Tom Lloyd (JHU Neurology). Finally, in collaboration with Janice Evans (Bloomberg School of Public Health), we found that these same principles apply to the development of a mammalian egg where disruption of the cell mechanics machinery causes defects in the formation of a healthy egg; such defects could be the cause of some types of human infertility and/or birth defects .
We are also leveraging our sophisticated understanding of cytokinesis, cell mechanics, and cellular mechanosensing to identify and develop small molecule modulators (i.e. possible future drugs) of cell mechanics. Such tools will be invaluable for dissecting tissue mechanics during normal development, tissue homeostasis, and pathological situations such as tumor formation and metastasis. To identify and develop small molecule modulators of cell mechanics, we draw upon the fact that cytokinesis in the simple amoeba Dictyostelium discoideum is exquisitely sensitive to changes in cell mechanics and that perturbation of these mechanics leads to an easily measurable phenotype, which is the formation of multinucleated cells. Towards this goal, we developed an automated image analysis platform called CIMPAQ for high-throughput drug screening for such modulators. In initial experiments, we identified a novel compound, carbamate-7, which shifts one of the major cell mechanics proteins myosin II into the cell skin (cell cortex) where it increases cortical tension and elasticity. We are testing the active component of this compound in several of the disease systems described above and already have found that the compound can correct the aberrant mechanics of metastatic cells. With Bob Anders, we will soon initiate animal studies to test the ability of the compound to alter the trajectory of metastatic disease.
Overall, our program seeks to determine how cells and tissues integrate chemical and mechanical information flow for normal tissue growth and homeostasis with the ultimate goal of being able to guide these processes with small molecules for therapeutic purposes. Starting from a simple model system, we are able to uncover and dissect fundamental principles of cell biology that would be next to impossible to discover in more complex situations. Then, by knowing what to look for, we find the same principles underly various diseases, which we are then working to correct with small molecules (potential future drugs).
A Few Key Papers
1. Zhang, W., and Robinson, D.N. (2005). Balance of actively generated contractile and resistive forces controls cytokinesis dynamics. Proc. Natl. Acad. Sci. U.S.A. 102, 7186-7191.
2. Reichl, E.M., Ren, Y., Morphew, M.K., Delannoy, M., Effler, J.C., Girard, K.D., Divi, S., Iglesias, P.A., Kuo, S.C., and Robinson, D.N. (2008). Interactions between myosin and actin crosslinkers control cytokinesis contractility dynamics and mechanics. Curr. Biol. 18, 471-480.
3. Effler, J.C., Kee, Y.S., Berk, J.M., Tran, M.N., Iglesias, P.A., and Robinson, D.N. (2006). Mitosis-specific mechanosensing and contractile-protein redistribution control cell shape. Curr Biol 16, 1962-1967.
4. Ren, Y., Effler, J.C., Norstrome, M., Luo, T., Firtel, R.A., Iglesias, P.A., Rock, R.S., and Robinson, D.N. (2009). Mechanosensing through cooperative interactions between myosin II and the actin crosslinker cortexillin. Curr Biol 19, 1421-1428.
5. Luo, T., Mohan, K., Srivastava, V., Ren, Y., Iglesias, Pablo A., and Robinson, Douglas N. (2012). Understanding the cooperative interaction between myosin II and actin cross-linkers mediated by actin filaments during mechanosensation. Biophys. J. 102, 238-247.
6. Luo, T., Mohan, K., Iglesias, P.A., and Robinson, D.N. (2013). Molecular mechanisms of cellular mechanosensing. Nat. Mater. 12, 1064-1071.
7. Kee, Y.S., Ren, Y., Dorfman, D., Iijima, M., Firtel, R.A., Iglesias, P.A., and Robinson, D.N. (2012). A mechanosensory system governs myosin II accumulation in dividing cells. Mol Biol Cell 23, 1510-1523.
8. Poirier, C.C., Ng, W.P., Robinson, D.N., and Iglesias, P.A. (2012). Deconvolution of the cellular force-generating subsystems that govern cytokinesis furrow ingression. PLoS Comput Biol 8, e1002467.
9. Sun, Q., Luo, T., Ren, Y., Florey, O., Shirasawa, S., Sasazuki, T., Robinson, D.N., and Overholtzer, M. Competition between human cells by entosis. Cell Res. Submitted.
10. Sidhaye, V.K., Chau, E., Srivastava, V., Aggarwal, N.R., D'Alessio, F.R., Robinson, D.N., and King, L.S. (2012). A novel role for aquaporin-5 in enhancing microtubule organization and stability. PLoS ONE 7, e38717.
11. Larson, S.M., Lee, H.J., Hung, P.H., Matthews, L.M., Robinson, D.N., and Evans, J.P. (2010). Cortical mechanics and meiosis II completion in mammalian oocytes are mediated by myosin-II and Ezrin-Radixin-Moesin (ERM) proteins. Mol Biol Cell 21, 3182-3192.