Developmental phenotype in the presence of cAMPgS

Cells are harvested from a suspension culture, washed and resuspended in the developmental buffer (DB: 2 mM MgCl₂, 0.2 mM CaCl₂ in 10 mM Na/K phosphate pH 6.5) at 5x10⁶ cells /ml. 5 ml cells are then spotted on DB agar containing cAMPγS at concentrations of 0, 10^-6, 10^-5 M. cAMPγS should be mixed with the melted DB agar when the agar is at about 40 ^oC. Terminal phenotypes can be observed within 36 hours.

This page was last edited 06/26/2003