Confocal imaging. We have implemented systems for point scanning, line-scanning, and spinning disk confocal microscopy. We also worked with systems for 2P microscopy, structured illumination imaging, deconvolution processing, side plane illumination microscopy (SPIM), TIRF, and PALM for critical display and resolution of focal plane details.
High-throughput scanning with image montage. We have implemented solutions for displaying GFP expression in entire mouse brains contained in sections. Each section is photographed with a precision stage and high NA objective to allow precise stitching in creating an image montage. Special software is used to register sections and create a detailed 3D-volume view.
Time-lapse fluorescence imaging. We assembled two wide field fluorescence microscope systems for fluorescence wide field time-lapse imaging. The Olympus IX and Nikon Ti2000 microscopes are fitted with external shutters, filters, autofocus controls, and environmental chambers for precision cell monitoring.
Murphy, D.B. (2001) Fundamentals of Light Microscopy and Electronic Imaging. John Wiley & Sons, N.Y.
Xiao, Z., Zhang, N., Murphy, D.B., and Devreotes, P.N., 1997. Dynamic Distribution of chemoattractant rece3ptors in Living Cells During Chemotaxis and Persisten Stimulation. J. Cell Biol., 139, 365-74.
Parent, C.A., Blacklock, B.J., Froehlich, W.M., Murphy, D.B., and Devreotes, P.N., 1998. G-Protein Signaling Events are Activated at the Leading Edge of Chemotactic Cells. Cell 95, 81-91.
Kaiser, D.A., Vinson, V.K., Murphy, D.B., and T.D. Pollard, 1999. Profilin is predominantly associated with monomeric actin in Acanthamoeba. J Cell Sci. 112, 3779-90.
Reyes, J.M., Fermanian, S., Yang, F., Zhou, S.Y., Herretes, S., Murphy, D.B., Elisseeff, J.H., and Chuck, R.S., 2006. Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy. Stem Cells 24, 1213-7.
Cohen, D.M., Kutscher, B., Chen, H., Murphy, D.B., and Craig, S.W., 2005. A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. J. Biol. Chem. 281, 16006-15.