CELL BIOLOGY Home Faculty Department Directory Seminars EducationNews Links
Adjunct Faculty  
Douglas B. Murphy, Ph.D.
Director, Optical and Electron Microscopy Laboratory
HHMI Janelia Farm Research Campus
19700 Helix Dr.
Ashburn, VA 20147
Telephone: (Office)571-209-4676
Fax: N/A
Email: murphyd@janelia.hhmi.org
Website:


 

   

I am presently Director of light microscopy, histology, and cell culture at the new HHMI Janelia Farm Research Campus in Virginia.  The facilities provide equipment, training and services in histology and fluorescence imaging. In Virginia, we have implemented methods and equipment for the tiling and photomontage of large fluorescent sections, super-resolution methods involving photoactivation localization microscopy (PALM) and structured illumination (SI) imaging, advanced fluorescence techniques (FRET, FRAP, TIRF), confocal microscopy and 2-photon imaging, and time-lapse imaging with environmental control. I have been a significant contributor to review articles and Java tutorials on the Olympus optical microscopy educational websites and in 2001 wrote a textbook entitled Fundamentals of Light Microscopy and Electronic Imaging. In past years at Hopkins my lab investigated the role of microtubules in organelle transport and cell motility; microtubule polymer dynamics; and the functions of microtubule-associated proteins such as MAP-2 in neurons and other cell types.

Confocal imaging.  We have implemented systems for point scanning, line-scanning, and spinning disk confocal microscopy.  We also worked with systems for 2P microscopy, structured illumination imaging, deconvolution processing, side plane illumination microscopy (SPIM), TIRF, and PALM for critical display and resolution of focal plane details. 

High-throughput scanning with image montage.  We have implemented solutions for displaying GFP expression in entire mouse brains contained in sections.  Each section is photographed with a precision stage and high NA objective to allow precise stitching in creating an image montage.  Special software is used to register sections and create a detailed 3D-volume view.

Time-lapse fluorescence imaging.  We assembled two wide field fluorescence microscope systems for fluorescence wide field time-lapse imaging. The Olympus IX and Nikon Ti2000 microscopes are fitted with external shutters, filters, autofocus controls, and environmental chambers for precision cell monitoring.

Selected Publications

Murphy, D.B. (2001) Fundamentals of Light Microscopy and Electronic Imaging. John Wiley & Sons, N.Y.
Xiao, Z., Zhang, N., Murphy, D.B., and Devreotes, P.N., 1997. Dynamic Distribution of chemoattractant rece3ptors in Living Cells During Chemotaxis and Persisten Stimulation. J. Cell Biol., 139, 365-74.

Parent, C.A., Blacklock, B.J., Froehlich, W.M., Murphy, D.B., and Devreotes, P.N., 1998. G-Protein Signaling Events are Activated at the Leading Edge of Chemotactic Cells. Cell 95, 81-91.

Kaiser, D.A., Vinson, V.K., Murphy, D.B., and T.D. Pollard, 1999. Profilin is predominantly associated with monomeric actin in Acanthamoeba. J Cell Sci. 112, 3779-90.

Reyes, J.M., Fermanian, S., Yang, F., Zhou, S.Y., Herretes, S., Murphy, D.B., Elisseeff, J.H., and Chuck, R.S., 2006. Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy. Stem Cells 24, 1213-7.

Cohen, D.M., Kutscher, B., Chen, H., Murphy, D.B., and Craig, S.W., 2005. A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. J. Biol. Chem. 281, 16006-15.

 

return to top

Updated: 2/2/11

**This site best when viewed in Mozilla Firefox**
 
Copyright © 2009 Johns Hopkins University Department of Cell Biology
All rights reserved. Contact webmaster