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Huang, Shau-Ku, Ph.D.

Research interests: Immunoregulation and molecular genetics of allergic diseases

The pathophysiology of allergic diseases, including asthma, is multifactorial and involves intricate networks of interactions between cells, mediators and cytokines. At present, the molecular genetic basis and the precise sequence of events leading to the inflammatory responses and tissue hyperreactivity are as yet to be determined. Further, the regulatory mechanisms of allergen-specific immunotherapy, the most effective form of therapy, remain elusive. To this end, we are currently pursuing three main projects: (i) Immunoregulation of antigen-induced allergic responses; we are combining genomics, molecular and cellular approaches, as well as mouse models, to uncover the regulatory mechanisms of allergic inflammation and allergen-specific immunotherapy, and (ii) Molecular genetic studies of allergic diseases; we are conducting genetic mapping analyses and positional cloning studies of susceptibility genes for asthma and asthma-associated phenotypes. To complement the genetic mapping effort and to sort out the complexities of variable molecular and cellular interactions, we are also conducting a series of functional genomics studies to identify and characterize differentially expressed genes in an effort to define differential and/or dysregulated expression of the allergic inflammatory gene network. Immunoregulation and molecular genetics of allergic diseases

Current research

The pathophysiology of allergic diseases, including asthma, is multifactorial and involves intricate networks of interactions between cells, mediators and cytokines. At present, the molecular genetic basis and the precise sequence of events leading to the inflammatory responses and tissue hyperreactivity are as yet to be determined. Further, the regulatory mechanisms of allergen-specific immunotherapy, the most effective form of therapy, remain elusive. To this end, we are currently pursuing three main projects: (i) Immunoregulation of antigen-induced allergic responses; we are combining genomics, molecular and cellular approaches, as well as mouse models, to uncover the regulatory mechanisms of allergic inflammation and allergen-specific immunotherapy, and (ii) Molecular genetic studies of allergic diseases; we are conducting genetic mapping analyses and positional cloning studies of susceptibility genes for asthma and asthma-associated phenotypes. To complement the genetic mapping effort and to sort out the complexities of variable molecular and cellular interactions, we are also conducting a series of functional genomics studies to identify and characterize differentially expressed genes in an effort to define differential and/or dysregulated expression of the allergic inflammatory gene network.

The pathophysiology of allergic diseases, including asthma, is multifactorial and involves intricate networks of interactions between cells, mediators and cytokines. At present, the molecular genetic basis and the precise sequence of events leading to the inflammatory responses and tissue hyperreactivity are as yet to be determined. Further, the regulatory mechanisms of allergen-specific immunotherapy, the most effective form of therapy, remain elusive. To this end, we are currently pursuing three main projects: (i) Immunoregulation of antigen-induced allergic responses; we are combining genomics, molecular and cellular approaches, as well as mouse models, to uncover the regulatory mechanisms of allergic inflammation and allergen-specific immunotherapy, and (ii) Molecular genetic studies of allergic diseases; we are conducting genetic mapping analyses and positional cloning studies of susceptibility genes for asthma and asthma-associated phenotypes. To complement the genetic mapping effort and to sort out the complexities of variable molecular and cellular interactions, we are also conducting a series of functional genomics studies to identify and characterize differentially expressed genes in an effort to define differential and/or dysregulated expression of the allergic inflammatory gene network.

Representative recent publications

Krishnendu, R., Mao, H.Q., Huang, S.K., and Leong, K.W., Oral gene delivery with chitosan-DNA nanoparticles generate immunologic protection in a murine model of peanut allergy. Nature Med., 5, 387, 1999.

Kawaguchi, M, L.F. Onuchic, X.D. Li, D.M. Essayan, J. Schroeder, H.Q. Xiao, M.C. Liu, G. Krishnaswamy, G. Germino, and S.K. Huang. Identification of a novel cytokine, ML-1, and its expression in subjects with asthma. J. Immunol. 167: 4430-5, 2001.

Chen, L.C., Z. Zhang, A.C. Myers, and S.K. Huang. Cutting Edge: Altered pulmonary eosinophilic inflammation in mice deficient for Clara cell secretory 10-kDa protein. J. Immunol. 267:3025, 2001.

Kawaguchi M, Onuchic LF, Huang SK. Activation of extracellular signal-regulated kinase (ERK)1/2, but not p38 and c-Jun N-terminal kinase, is involved in signaling of a novel cytokine, ML-1. J Biol Chem. 277:15229, 2002.

Hung CH, Chen LC, Zhang Z, Hung CH, Plunkett B, Myers AC, and Huang SK. Regulation of Th2 responses by the pulmonary Clara cell secretory 10-kDa protein (CC10). J. Allergy and Clin. Immunol., 114:664, 2004.

Oda, N, PB Canelos, DM Essayan, BA Plunkett, AC Myers and SK Huang. Interleukin-17F induces neutrophilia and amplifies antigen-induced allergic response. Am. J. Respir. Cri. Care Med., 171:12, 2005.

Konno S, Golden DB, Schroeder J, Hamilton RG, Lichtenstein LM, Huang SK. <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15867867> Increased expression of osteopontin is associated with long-term bee venom immunotherapy. J Allergy Clin Immunol., 115:1063, 2005.

Huang, SK, RA Rasika, E Ehrlich, B Plunkett, X Liu, XD Li, F Malveaux, G Dunston, C Ober, M Blumenthal, E Bleecker, S Rich, T Beaty and CSGA. Evidence for asthma susceptibility loci on Chromosome 11 in an African American population. Hum Genet, 113:71, 2003.

Gao PS, Heller NM, Walker W, Chen CH, Plunkett B, Roberts MH, Schleimer RP, Hopkin JM, and Huang SK. Variation in dinucleotide (GT) repeat sequence in the first exon of the STAT6 gene is associated with high total serum IgE level and differentially regulates the promoter activity in vitro. J. Med. Genet., 41:535, 2004. Principal Investigator., Postdoctoral Fellow (2004-present), Postdoctoral Fellow (2004-present), Postdoctoral Fellow (2004- present)Senior Research Technician Student (Howard Hughes Undergraduate Student Fellow; 2000-present), University of Maryland, College Park; since 1995, practical training

Huang JL, Gao PS, Mathias RA, TC Yao, LC Chen, ML Kuo, Hsu SC, Plunkett B, Togias, A, Barnes KC, Stellato C, Beaty TH and Huang SK. Sequence variants of the gene encoding chemoattractant receptor expressed on Th2 cells (CRTH2) is associated with asthma and differentially influence the mRNA stability. Hum. Mol. Genet., 13:2691, 2004.

Gao PS, Mathias RA, Plunkett B, Togias A, Barnes KC, Beaty TH, Huang SK. <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15867855> Genetic variants of the T-cell immunoglobulin mucin 1 but not the T-cell immunoglobulin mucin 3 gene are associated with asthma in an African American population. J Allergy Clin Immunol., 115:982, 2005.

Current laboratory personnel (since May 1, 2005)

Shau-Ku Huang, Ph.D., Principal Investigator

Chih-Feng Chian, M., Postdoctoral Fellow (2004-present)

Masatsugu Kurokawa, MD PhD, Postdoctoral Fellow (2004-present)

Qiu Liu, MD PhD, Postdoctoral Fellow (2004- present)

Beverly Plunkett, MS, Senior Research Technician

Shih-Chang Hsu, Student (Howard Hughes Undergraduate Student Fellow; 2000-present), University of Maryland, College Park; since 1995, practical training

Molecular genetics:

Immunoregulation:

 
 
 
 
 

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