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Recent Posters

(updated 11/09/2015)
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(Presented at NIH-IEEE 2015 Strategic Conference on Healthcare Innovations and Point-of-Care Technologies for Precision Medicine, Bethesda, MD, November 9-10, 2015)
SYSTEMS ENGINEERING AND POINT OF CARE TESTING: Report from the NIBIB POCTRN Systems Engineering Workshop
Stahl JE, McGowan H, DiResta E, Gaydos CA, Klapperich C, Parrish J, Carleton P, Korte B.
Conclusion: POCT has the potential to profoundly shape future healthcare delivery. These technologies, however, have the potential to radically alter where, when and how care is delivered and therefore they need to be understood from a systems perspective to best allow their and the system's success.

►► View PDF of poster here.

NIBIB System Engineering

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(Presented at 29th IUSTI European Conference on STIs, Barcelona, Spain, September 24-26, 2015)
Can residual urethral swabs be used for detection of Neisseria gonorrhoeae and antimicrobial resistance markers?
Melendez JH, Hardick J, Barnes M, Barnes P, Geddes CD, Gaydso CA.
Conclusions: Sensitivity of PCR for detection of GC and resistance markers in residual urethral swabs was low; use of a separate swab for PCR-based analysis may increase sensitivity.

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Residual urethral swabs

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(Presented at 2015 World STI & HIV Congress, Brisbane, Australia, September 13-16, 2015)
Utilization of risk score tool of Internet I Want the Kit (IWTK) home self-collection program for STIs in males
Hsieh YH, Jett-Goheen M, Dize L, Barnes M, Barnes P, Gaydos CA.
Conclusions: After excluding potential selection bias, IWTK risk score tool predicted the presence of STIs for male users and could potentially be used for identifying hotspots for STI intervention.

►► View PDF of poster here.

IWTK Risk Score in Males_ISSTDR 2015

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(Presented at 2015 World STI & HIV Congress, Brisbane, Australia, September 13-16, 2015)
An agent-based model to simulate and predict HIV epidemic in Baltimore City, Maryland, USA
Adleberg J, Rothman R, Flynn C, Nganga-Good C, Hsieh YH.
Conclusions: Agent-based models can capture elements of the complexity inherent in infectious disease transmission.

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An agent-based model to simulate and predict HIV epidemic

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(Presented at 115th General Meeting of American Society for Microbiology, New Orleans, LA, May 30-June 2, 2015)
Ultra-Rapid Isolation and Detection of Neisseria gonorrhoeae by Microwave-Accelerated Methods
Melendez JH, Gaydos CA, Hardick J, Geddes CD.
Conclusions: Microbial lysing and DNA fragmentation can be achieved in al little as 30 seconds by microwave irradiation. Microwave-based DNA isolation is cheaper and faster than chemical lysing approaches. Microwave-accelerated methods have the potential to be useful in the development of POC diagnostics.

►► View PDF of poster here.

Melendez ASM2015

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(Presented at 31st Clinical Virology Symposium, Daytona Beach, FL, April 26-29, 2015)
Can a Rapid Point-of-Care Assay be Used to Diagnose Both HIV and Syphilis? Evaluation of the SD BIOLINE HIV/Syphilis Duo Test
Holden J, Barnes M, Barnes P, Goheen J, Hsieh YH, Marsiglia V, Duncan D, Gaydos C.
Conclusions: The HIV component of the SD Duo performed well. However, our results for the syphilis component, when compared to TPPA and RPR, were less than optimal and support the need for further testing and assessment.

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Holden CVS 2015 poster

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(Presented at 31st Clinical Virology Symposium, Daytona Beach, FL, April 26-29, 2015)
Performance of the AmpliVue® Trichomonas Assay for the Detection of Trichomonas vaginalis in Women from Vaginal Specimens
Gaydos CA, Hobbs M, Marrazzo J, Masek B, Barnes M, Dize L, Cherneski M.
Conclusion: This study provides evidence of new diagnostic options and indicates better performance of amplified testing for detection of TV in symptomatic and asymptomatic women.

►► View PDF of poster here

Gaydos CVS 2015 poster

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(Presented at 31st Clinical Virology Symposium, Daytona Beach, FL, April 26-29, 2015)
Will Men Collect a Penile-Meatal Swab for the Diagnosis of Sexually Transmitted Infections in a Clinical Setting?
Barnes P, Barnes M, Hsieh YH, Dize L, Gaydos C.
Conclusion: Self-collected penile specimens were the preferred choice of sample type for all men in the study.

►► View PDF of poster here

Barnes_CVS 2015 poster

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(Presented at 31st Clinical Virology Symposium, Daytona Beach, FL, April 26-29, 2015)
Will Self-Collected Penile Swabs Perform as Well as Clinician-Collected Urethral Swabs for the Detection of Sexually Transmitted Infections Such as Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis and Mycoplasma genitalium?
Dize L, Barnes M, Barnes P, Hsieh YH, Marsiglia V, Duncan D, Gaydos C.
Conclusions: Self-collected penile swabs appeared to be as accurate as clinician-collected swabs for the detection of STIs by a NAAT assay and could expedite express visits in a busy STD clinic.

►► View PDF of poster here

Dize CVS 2015 poster

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(Presented at Molecular Diagnostics Europe Conference, Lisbon, Portugal, April 13-16, 2015)
A clinical evaluation of a fully-Integrated, Low-Cost, Ultra-Rapid PCR Device with Point-of-Care Applications for Chlamydia trachomatis and Neisseria gonorrhoeae
Ferrao C, Dixon A, Hardick J, Adlerstein D, Gaydos C.
Conclusion: This sensitive, low-cost, rapid PCR POC device could impact management and treatment of CT and NG infections by reducing time to treatment.

►► View PDF of poster here.

Atlas Genetics 2015 poster

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(Presented at 13th International Symposium on Human Chlamydial Infections, Pacific Grove, CA, June 22-27, 2014)
Evaluation of Pooled Ocular and Vaginal Swabs by the Cepheid GeneXpert CT/NG Assay for the Detection of Chlamydia trachomatis as Compared to the APTIMA Combo2 Assay
Dize L, West SK, Mkocha H, Quinn TC, Gaydos CA.
Conclusions: As compared to the AC2 assay, the GeneXpert assay shows promise as a rapid, near patient PCR test, which demonstrates high sensitivity and specificity. Cost savings depends on prevalence in the population; as prevalence decreases, pool size can increase, resulting in an increase in cost-savings.

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Eval of Pooled Swabs

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
What do Women Patients Expect from Use of Point-of-Care Tests (POCT) for STDs in a Clinic?
Barnes M, Hsieh YH, Jett-Goheen M, Gaydos CA.
Conclusions: Self-collected vaginal specimens were the preferred and easiest specimen choice for POCT. Most women preferred to only wait 20-40 minutes for their results. Women were evenly divided as to home or clinic for rescreening site and most were willing to pay only $10-20 for a POCT if one were available OTC.

►► View PDF of poster here

POCTs Women Clinic

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
Ultra Rapid and Sensitive Detection of Neisseria gonorrhoeae and Antimicrobial Resistance Susceptibility
Melendez JH, Gaydos CA, Geddes CD.
Conclusions: Our lysing approach can effectively lyse cells and fragment DNA. Dry vaginal swabs are an effective collection method for MAMEF testing. Sensitivity and specificity of the MAMEF assay is high.

►► View PDF of poster here

NG MAMEF poster

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
I Want the Kit (IWTK) - What's Your Risk for Having a Sexually Transmitted Infection (STI)?
Jett-Goheen M, Barnes M, Dize L, Barnes P, Hsieh YH, Gaydos CA.
Conclusions: Risk scores may predict the likelihood of a person having a sexually transmitted infection. The score may help an IWTK user decide whether to order a test kit.

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IWTK Risk Score poster

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
Acceptability and Perceived Accuracy of Rapid and Standard Sexually Transmitted Infection (STI) Screening and Self-Collection of Samples on a Mobile Health Van
Patterson-Rose S, Hesse E, Gaydos C, Widdice L.
Conclusion: Community event attendees reported STI screening on health vans using rapid or standard tests with urine or self-collected swabs to be acceptable and accurate.

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Widdice 2014 acceptabiltiy

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
Feasibility of screening and follow-up for sexually transmitted infections (STIs) using rapid and standard STI testing from a mobile health van
Patterson-Rose S, Hesse E, Dize L, Gaydos C, Widdice L.
Conclusions: Men and women will seek STI services at mobile health vans at public events. Rapid tests facilitate successful immediate treatment of positive STI results.

►► View PDF of poster here

Widdice 2014 poster

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
First Year of Usage of So They Can Know, an STD Partner Notification Website
Ladd JH, McManus JO, Barclay L, Caydos C.
Conclusion:  So They Can Know has seen an increase in monthly users over time and an increase in the proportion of users accessing content about STDs or partner notification.

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Ladd 2014 poster

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
Scope of Practice for Reproductive Health: Assuring a Trained Medical Workforce in the Era of the Affordable Care Act
Hogan T, Smith T, Arbar M, Scott LT, Mason C, Hoover J, Marcell AV, Eikner D, Rompalo AM, McKenna R.
Background: In 2010, the Male Training Center for Family Planning and Reproductive Health (MTC), a partnership between the Family Planning Council and Johns Hopkins Medicine, conducted a survey of licensing boards. The survey determined whether providers specializing in  female healthcare were within their scope of practice when offering reproductive healthcare services to males in Title X settings.

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Hogan 2014 poster

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(Presented at 2014 CDC STD Prevention Conference, Atlanta, GA, June 9-12, 2014)
STI and HIV Screening in Older Adults: An Integrative Literature Review
Tillman JL, Mark HD.
Conclusions: There are missed opportunities to identify STIs and HIV in older adults. Health care providers must overcome assumptions and stereotypes about older adult sexuality and utilize their influence to encourage STI and HIV testing among their older adult patients.

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Tillman_2014_poster

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(Presented at American Public Health Association, Boston, MA, November 2-6, 2013)
Marital Status and Sexually Transmitted Infection Risk Among Non-Injection and Injection Drug Users
Khan MR, Scheidell JD, Gaydos C, Coatsworth AM, Latimer WW.
Conclusion: The results highlight the importance of STI screening and treatment in the drug-using population, not only among single individuals but also married individulas and their partners.

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Marital Status and STI Risk

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(Presented at the STI & AIDS World Congress 2013, Vienna, Austria, July 14-17, 2013)
What qualities do providers identify as best for Point of Care STI Tests and do opinions differ by practice, region and country?
Rompalo A, Hsieh YH, Guy R, Natoli L, Fairley K, Gupta S, Radcliffe K, Hogan MT, Jett-Goheen M, Gaydos C.
ConclusionsPriorities for POCT development differ among clinicians from developing vs. developed countries. Participants from developing countries ranked early HIV seroconversion as top priority while those from developed countries chose chlamydia. Participants from developing countries consistently chose test that were less expensive compared to those from developed countries who chose tests that were more expensive but had higher sensitivity and specificity.

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Romaplo_Qualities Providers Identify

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(Presented at the STI & AIDS World Congress 2013, Vienna, Austria, July 14-17, 2013)
Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF) Point-of-Care Test for the Detection of Chlamydia trachomatis
Melendez JH, Huppert J, Hesse E, Jett-Goheen M, Quinn N, Gaydos CA, Geddes CD.
ConclusionsMAMEF is an ultra-fast and sensitive approach for detection of CT. Our CT assay can specifically detect over 80% of cases in less than 10 minutes. Dry vaginal swabs are an effective collection method for MAMEF testing.

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MAMEF POCT for CT

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(Presented at the STI & AIDS World Congress 2013, Vienna, Austria, July 14-17, 2013)
Copan ESwabTM, a liquid based microbiology device, can be used for the preservation of Neisseria gonorrhoeae for culture and for detection of CT/NG by Gen-Probe® APTIMA® COMBO 2 Assay
Jett-Goheen M, Barnes M, Duncan D, Quinn N, Ghanem K, Gaydos C.
ConclusionsThe ability of ESwabTM to maintain culture viability of NG up to 24 hours until NAAT testing is completed could eliminate the necessity to perform NG cultures on all patients. ESwabTM can be used for collecting the initial sample, for testing by NAAT assay, and then NG culture can be performed for susceptibility testing for all NG NAAT+ patients.

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Goheen_Copan ESwab for NG

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(Presented at the STI & AIDS World Congress 2013, Vienna, Austria, July 14-17, 2013)
An easy to use real time PCR test for Chlamydia trachomatis and Neisseria gonorrhoeae providing results that can guide treatment choice before the patient leaves the clinic
Gaydos CA, Van Der Pol B, Jett-Goheen M, Barnes M, Quinn N, Clark C, Daniel GE, Dixon PB, Hook EW III.
ConclusionsThe GeneXpert® System is a closed, self-contained, fully-integrated, automated platform for CT/NG testing that demonstrated excellent sensitivities and specificities in women and men. This relatively rapid short-turn-around-test can provide results to guide treatment decisions before patients leave the clinical setting. Such rapid treatment could potentially improve chlamydia and gonorrhea control efforts.

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Gaydos_East to use real time PCR test for CT and NG

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(Presented at the STI & AIDS World Congress 2013, Vienna, Austria, July 14-17, 2013)
Novel emergency department registration kiosk for HIV screening increases engagement of high risk patients and is cost-effective
Hsieh YH, Peterson S, Gauvey-Kern M, Gaydos CA, Holtgrave D, Rothman RE.
ConclusionsED kiosk-based HIV screening resulted in testing significantly greater portions of patients with 'high risk' behaviors and yielded higher rates of newly identified HIV+ patients. Use of kiosks for offering HIV screening was potentially more cost-effective than a counselor-based bedside approach per new diagnosis identified.

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Hsieh_ED Kiosk for HIV Screening

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(Presented at the STI & AIDS World Congress 2013, Vienna, Austria, July 14-17, 2013)
Initial Uptake of STI Partner Notification Website So They Can Know
Ladd JH, McManus JO, Rushanan M, Adelson S, Barclay L, Gaydos C.
ConclusionsWith little active promotion through clinics or online search optimization, few visitors to So They Can Know have used the website for anonymous partner notification or for misuse. Active promotion will be studied in the future.

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Ladd_So They Can Know_ISSTDR 2013

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(Presented at the 2013 Annual Meeting of the Society for Academic Emergency Medicine, Atlanta, GA, May 15-18, 2013)
Factors Associated with Patients Declining Kiosk-Facilitated HIV Self-Testing in the Emergency Department
Beck KJ, Gauvey-Kern M, Woodfield A, Peterson S, Gaydos CA, Rothman RE, Hsieh YH.
Conclusions: New operational strategies to integrate HIV testing into EDs are evolving, and now include ED-based kiosk-facilitated self-testing. Further study is needed to identify patients' reasons for declining self-testing and to further optimize this approach to ED-based HIV testing.

►► View PDF of poster here.

Patients Declining HIV Self testing

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(Presented at the 29th Annual Clinical Virology Symposium, Daytona Beach, FL, April 28 - May 1, 2013)
Performance of a New Rapid Real-Time PCR Test for Chlamydia trachomatis and Neisseria gonorrhoeae Which Can Be Used to Provide Results for Patients While in a Clinical Setting
Gaydos CA, Van Der Pol B, Jett-Goheen M, Barnes M, Quinn N, Clark C, Daniel G, Dixon P, Hook EW III.
ConclusionsThe GeneXpert® System is a rapid, self-contained, automated platform for CT/NG testing that demonstrated excellent sensitivities and specificities in symptomatic and asymptomatic women and men. The Xpert assay can provide results to guide treatment decisions before patients leave the doctors office, clinic, emergency department, or in many other settings, such as potentially even a pharmacy.

►► View PDF of poster here.

Cepheid GeneXpert CTNG

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(Presented at the 29th Annual Clinical Virology Symposium, Daytona Beach, FL, April 28 - May 1, 2013)
Performance of a New Amplified DNA Assay on the BD ViperTM System in Extracted Mode for the Detection of Trichomonas vaginalis in Women from Cervical, Vaginal, and Urine Specimens.
Gaydos CA, Williams JA, Quinn N, Eddleman L, Fuller D, Taylor S, Schwebke J, Lebed J, Smith B, Body B, Nye M, Van Der Pol B.
Conclusion: This study provided evidence for adoption and use of new diagnostic options by laboratories and indicated better performance of amplified testing for detection of trichomonas compared to older type tests.

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BD Viper Trich poster

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(Presented at 2012 National Summit on HIV and Viral Hepatitis Diagnosis, Prevention, and Access to Care, Washington DC, November 26-28, 2012)
Self-testing in the Emergency Department (ED) by Kiosk
Gaydos C, Solis M, Hsieh YH, Nour S, Jett-Goheen M, Rothman R.
Conclusions: Kiosk initiated testing proved to be highly feasible, acceptable, and accurate method of conducting rapid HIV self-testing in this pilot study; however, rates of engagement were only moderate, with almost half of patients volunteering to perform an HIV test.

View PDF of poster here.

Gaydos_SelfTesting by Kiosk_poster

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(Presented at 2012 National Summit on HIV and Viral Hepatitis Diagnosis, Prevention, and Access to Care, Washington DC, November 26-28, 2012)
Kiosk-Facilitated Patient Self Testing for HIV in an Emergency Department Rapid HIV Screening Program
Hsieh YH, Gauvey-Kern M, Gaydos CA, Woodfield A, Peterson S, Rothman RE.
Conclusions: This is the first demonstration of kiosks for engaging patients and guiding HIV self-testing in a health care setting. Notably, while rates of agreement for self-testing were similar to facilitator testing, more than half of patients declined to perform their own test; further, a substantial number of patients needed assistance from staff or failed to complete self-testing.

►► View PDF of poster here.

Hsieh_NationalSummit_KioskSelfTesting_poster

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(Presented at 2012 National Summit on HIV and Viral Hepatitis Diagnosis, Prevention, and Access to Care, Washington DC, November 26-28, 2012)
Novel Emergency Department Registration Kiosk for HIV Screening Increases Engagement of High Risk Patients
Hsieh YH, Gauvey-Kern ME, Woodfield A, Peterson S, Wu L, Gaydos C, Rothman RE.
Conclusions: Streamlined upfront HIV screening via kiosk resulted in increased rates of HIV test offering and comparable testing completion rates relative to the bedside testing method. Patients rated kiosk-based approach as highly acceptable.

►► View PDF of poster here.

Hsieh_NationalSummit_Kiosk poster

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 View archived posters here