Other publications on Keratins and/or the Skin (Postdoctoral Training 1998-92)

COULOMBE PA , Omary MB (2002). Hard and soft principles defining the structure, function and regulation of keratin intermediate filaments. Current Opinion in Cell Biology. In press (Febr. 2002).

Yamada S, Wirtz D., COULOMBE PA (2002). Pairwise assembly determines the intrinsic potential for self-organization and mechanical properties of keratin filaments. Molecular Biology of the Cell. In press (Jan. 1 issue).

COULOMBE PA , Ma L, Yamada S., Wawersik M (2001). Intermediate filaments at a glance. Journal of Cell Science. In press (Dec. 26 issue).

Bousquet, O., Ma, L., Yamada, S., Gu, C., Idei, T., Takahashi, K., Wirtz, D., COULOMBE PA (2001). The non-helical tail domain of keratin14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro. Journal of Cell Biology 155: 747-53.

Wawersik M, Mazzalupo, S, Nguyen D, COULOMBE PA (2001). Increased Levels of Keratin 16 Alters the Epithelialization Potential of Skin Keratinocytes In Vivo and Ex Vivo. Molecular Biology of the Cell 12: 3439-50

Ma L, Yamada S, Wirtz D, COULOMBE PA (2001). A "hot spot" mutation alters the mechanical properties of keratin filament networks. Nature Cell Biology 3: 503-6.

Mazzalupo S, COULOMBE PA (2001). A Reporter Transgene Based on a Human Keratin 6 Gene Promoter Is Specifically Expressed in the Periderm of Mouse Embryos. Mechanisms of Development 100: 65-69.

Wawersik MJ, COULOMBE PA (2000). Forced expression of keratin 16 alters adhesion and differentiation properties of mouse skin keratinocytes. Molecular Biology of the Cell 11: 3315-27.

COULOMBE PA, Bousquet O, Ma L, Yamada S, Wirtz D (2000). The "Ins" and "Outs" of intermediate filament organization. Trends in Cell Biology 10: 420-8.

COULOMBE PA, McGowan K (2000). Morphogenesis of Mammalian Skin. In: Cell Polarity: Frontiers in Molecular Biology. Edited by D.G. Drubin. Oxford University Press, Oxford, England.

Wong P, Colucci-Guyon E, Gu C, Takahashi K, Babinet C, COULOMBE PA (2000). Introducing a null mutation in the mouse K6a and K6b genes reveals their essential structural role in oral mucosa epithelia. Journal of Cell Biology 150: 921-8.

McGowan K, COULOMBE PA (2000). The type I keratin 17 is expressed in the context of hard epithelia: Implications for the molecular pathogenesis of pachyonychia congenita. Journal of Investigative Dermatology 114: 1101-8. **cover article.

Steinbock FA, Nikolic B, COULOMBE PA, Fuchs E, Traub P, Wiche G (2000). Dose-dependent linkage, assembly inhibition and disassembly of vimentin qand cytokeratin 5/14 filaments through plectin's intermediate filament binding domain. Journal of Cell Science 113: 483-91.

Takahashi K, COULOMBE PA, Miyachi Y (1999). Using transgenic mouse models to study the pathogenesis of keratin-based inherited skin diseases. Journal of Dermatological Sciences 21: 73-95.

Paladini RD, COULOMBE PA (1999). The functional diversity of epidermal keratins revealed by the partial rescue of the keratin 14 null phenotype by keratin 16. Journal of Cell Biology 146: 1185-1201 **cover article.

Ma L, Xu J, COULOMBE PA, Wirtz D (1999). Keratin filament suspensions show unique micromechanical properties. Journal of Biological Chemistry 274: 19145-51.

Fradette J, Germain L, Sessaiah P, COULOMBE PA (1998). The type I keratin 19 shows distinct and context-dependent properties. Journal of Biological Chemistry, 273, 36176-84.

Hutton E, Paladini, RD, Yu QC, Yen M, COULOMBE PA, Fuchs E (1998). Functional differences between keratins of stratified and simple epithelia. Journal of Cell Biology, 143, 487-99.

McGowan K, COULOMBE PA (1998). Onset of keratin 17 expression coincides with the definition of major epithelial lineages during skin development. Journal of Cell Biology, 143, 469-86.

Takahashi K, Yan B, Yamanishi K, Imamura S, COULOMBE PA (1998). The two functional keratin 6 genes of mouse are differentially regulated and evolved independently from their human orthologs. Genomics, 53, 170-183.

Munoz-Marmol AM, Strasser G, Isamat M, COULOMBE PA, Yang Y, Roca X, Vela E, Mate JL, Coll J, Fernandez-Figueras MT, Navas-Palacio JJ, Ariza A, Fuchs E (1998). A dysfynctional desmin mutation in a patient with severe generalized myopathy. Proceedings of the National Academy of Science (USA) 95, 11312-17.

Paladini RD, COULOMBE PA (1998). Directed expression of keratin 16 to the progenitor basal cells of transgenic mouse skin delays skin maturation. Journal of Cell Biology, 142, 1035-51.

Miller SJ, Burke EM, Rader MD, COULOMBE PA, Lavker RM (1998). Reconstitution of wounded porcine epidermis from follicular and apocrine stem cells. Journal of Investigative Dermatology, 110:13-19.

McGowan K, COULOMBE PA (1997). The wound repair-associated keratins 6, 16 and 17: Insights into the role of intermediate filaments in specifying keratinocyte cytoarchitecture. In: Subcellular Biochemistry vol. 31: Intermediate Filaments (Herrmann H, Harris R, Eds.). Plenum Press, New York, pp. 173-204.

COULOMBE PA (1997). Towards a molecular definition of keratinocyte activation following acute injury to stratified epithelia. Biochemical and Biophysical Research Communications 236: 231-8.

COULOMBE PA (1997). Transgenic mice as a model for the study of inherited skin bullous diseases. Ann. Dermatol. Venerol. 124: 197-204.

Wawersik M, Paladini RD, Noensie E, COULOMBE PA (1997). A proline residue in the a-helical rod domain of type I keratin 16 destabilizes keratin heterotetramers. Journal of Biological Chemistry 272: 32557-65.

Takahashi K, COULOMBE PA (1997). Defining a region of the human K6a gene that confers inducible expression in stratified epithelial of transgenic mice. Journal of Biological Chemistry 272: 10979-85.

Takahashi K, COULOMBE PA (1996). A mouse model with an inducible skin blistering phenotype. Proceedings of the National Academy of Science (USA) 93: 14776-81.

Bousquet O, COULOMBE PA (1996). The cytoskeleton: Missing links found ? Current Biology 6: 1563-66.

COULOMBE PA, Takahashi K (1996): The cellular mechanisms of skin keratinocyte migration into a wound site: An open question with important implications. Cell Vision - Journal of Analytical Morphology 3: 217-223.

Uttam J, Hutton E, COULOMBE PA, Anton-Lampbrecht I, Yu QC, Gedde-Dahl T, Fine JD, Fuchs E (1996): The genetic basis for epidermolysis bullosa simplex with mottled pigmentation. Proceedings of the National Academy of Science (USA) 93: 9079-84.

Paladini R, Bravo NS, Takahashi K, COULOMBE PA (1996). Onset ot re-epithelialization after skin injury correlates with a reorganization of keratin filaments in wound edge keratinocytes: Defining a potential role for keratin 16. Journal of Cell Biology 132: 381-98.

COULOMBE PA, Bravo NS, Paladini RD, Nguyen D, Takahashi K (1995): Over-expression of human keratin 16 produces a distinct skin phenotype in transgenic mice. Biochemistry and Cell Biology 73 (9-10): 611-18.

Paladini R, Takahashi K, Gant TM, COULOMBE PA (1995). cDNA cloning and bacterial expression of the human type I keratin 16. Biochemical and Biophysical Research Communications 215: 517-23.

Takahashi K, Paladini R, COULOMBE PA (1995). Cloning and characterization of multiple human genes and cDNAs encodinh highly-related type II keratin 6 isoforms. Journal of Biological Chemistry 270(31): 18581-18592.

Lowthert LA, Ku NO, Liao J, COULOMBE PA, Omary MB (1995): Empigen BB: A useful detergent for solubilization and biochemical analysis of keratins. Biochemical and Biophysical Research Communications 206: 370-379.

COULOMBE PA, Fuchs (1994): Molecular mechanisms of keratin gene disorders and other inherited bullous diseases of the skin. In: "Molecular Mechanisms in Epithelial Cell Junctions: from Development to Disease". S. Citi, Editor. R.G. Landes Company, Austin (TX). pp. 259-284.

Takahashi K, Folmer J, COULOMBE PA (1994): Increased expression of keratin 16 causes anomalies in cytoarchitecture and keratinization in transgenic mouse skin. Journal of Cell Biology 127: 505-520.

COULOMBE PA, Fuchs E (1993): Epidermolysis Bullosa Simplex. Seminars in Dermatology 12: 173-190.

COULOMBE PA (1993): Cellular and molecular biology of keratins: Beginning a new era. Current Opinion in Cell Biology 5: 17-29.