DNA Prep for microinjection
The following 2 kits are the most popular methods for preparing DNA for pronuclear microinjection:
QIAquick Gel Extraction Kit (70 bp – 10 kb)
QIAEX II Gel Extraction Kit (40 bp – 50 kb)
With both kits, use T-lowE (10 mM TRIS, 0.1 mM EDTA) which is available from the Transgenic Core for the final elution step. You may make your own T-lowE however the stock available from the Core has been embryo tested and is recommended.
Even with kits, we see variation in the purity of the DNA submitted. The purity of the DNA and the accuracy of the quantification are the 2 most important factors in the success of your injections. Even when using a kit, careful attention to details is essential!
For those who are opposed to the use of kits, here is an additional protocol:
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